PubMed:10089566 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10089566","sourcedb":"PubMed","sourceid":"10089566","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10089566","text":"Involvement of adenylate cyclase and p70(S6)-kinase activation in IL-10 up-regulation in human monocytes by gp41 envelope protein of human immunodeficiency virus type 1.\nOur previous results show that recombinant gp41 (aa565-647), the extracellular domain of HIV-1 transmembrane glycoprotein, stimulates interleukin-10 (IL-10) production in human monocytes. The signal cascade transducing this effect is not yet clear. In this study, we examined whether gp41-induced IL-10 up-regulation is mediated by the previously described synergistic activation of cAMP and NF-kappaB pathways. gp41 induced cAMP accumulation in monocytes in a time- and concentration-dependent manner and the adenylate cyclase inhibitor SQ 22536 suppressed gp41-induced IL-10 production in monocytes. In contrast, gp41 failed to stimulate NF-kappaB binding activity in as much as no NF-kappaB bound to the main NF-kappaB-binding site 2 of the IL-10 promoter after addition of gp41. We also examined the involvement of other signal transduction pathways. Specific inhibitors of p70(S6)-kinase (rapamycin), and Gi protein (pertussis toxin), prevented induction of IL-10 production by gp41 in monocytes, while inhibitors of the phosphatidylinositol 3-kinase (PI 3-kinase) (wortmannin) and mitogen-activated protein kinase (MAPK) pathway (PD 98059) did not. Thus HIV-1 gp41-induced IL-10 up-regulation in monocytes may not involve NF-kappaB, MAPK, or PI 3-kinase activation, but rather may operate through activation of adenylate cyclase and pertussis-toxin-sensitive Gi/Go protein to effect p70(S6)-kinase activation.","tracks":[{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":15,"end":32},"obj":"NP"},{"id":"C2","span":{"begin":37,"end":62},"obj":"NP"},{"id":"C3","span":{"begin":89,"end":104},"obj":"NP"},{"id":"C4","span":{"begin":108,"end":168},"obj":"NP"},{"id":"C5","span":{"begin":201,"end":229},"obj":"NP"},{"id":"C6","span":{"begin":231,"end":291},"obj":"NP"},{"id":"C7","span":{"begin":341,"end":356},"obj":"NP"},{"id":"C8","span":{"begin":582,"end":586},"obj":"NP"},{"id":"C9","span":{"begin":616,"end":625},"obj":"NP"},{"id":"C10","span":{"begin":761,"end":770},"obj":"NP"},{"id":"C11","span":{"begin":785,"end":789},"obj":"NP"},{"id":"C12","span":{"begin":947,"end":951},"obj":"NP"},{"id":"C13","span":{"begin":1153,"end":1157},"obj":"NP"},{"id":"C14","span":{"begin":1161,"end":1170},"obj":"NP"},{"id":"C15","span":{"begin":1372,"end":1381},"obj":"NP"},{"id":"C16","span":{"begin":1487,"end":1504},"obj":"NP"},{"id":"C17","span":{"begin":1559,"end":1584},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-appos","subj":"C6","obj":"C5"},{"id":"R2","pred":"coref-ident","subj":"C7","obj":"C3"},{"id":"R3","pred":"coref-ident","subj":"C8","obj":"C4"},{"id":"R4","pred":"coref-ident","subj":"C9","obj":"C7"},{"id":"R5","pred":"coref-ident","subj":"C10","obj":"C9"},{"id":"R6","pred":"coref-ident","subj":"C11","obj":"C8"},{"id":"R7","pred":"coref-ident","subj":"C12","obj":"C11"},{"id":"R8","pred":"coref-ident","subj":"C13","obj":"C12"},{"id":"R9","pred":"coref-ident","subj":"C14","obj":"C10"},{"id":"R10","pred":"coref-ident","subj":"C15","obj":"C14"},{"id":"R11","pred":"coref-ident","subj":"C16","obj":"C1"},{"id":"R12","pred":"coref-ident","subj":"C17","obj":"C2"}],"attributes":[{"subj":"C1","pred":"source","obj":"genia-medco-coref"},{"subj":"C2","pred":"source","obj":"genia-medco-coref"},{"subj":"C3","pred":"source","obj":"genia-medco-coref"},{"subj":"C4","pred":"source","obj":"genia-medco-coref"},{"subj":"C5","pred":"source","obj":"genia-medco-coref"},{"subj":"C6","pred":"source","obj":"genia-medco-coref"},{"subj":"C7","pred":"source","obj":"genia-medco-coref"},{"subj":"C8","pred":"source","obj":"genia-medco-coref"},{"subj":"C9","pred":"source","obj":"genia-medco-coref"},{"subj":"C10","pred":"source","obj":"genia-medco-coref"},{"subj":"C11","pred":"source","obj":"genia-medco-coref"},{"subj":"C12","pred":"source","obj":"genia-medco-coref"},{"subj":"C13","pred":"source","obj":"genia-medco-coref"},{"subj":"C14","pred":"source","obj":"genia-medco-coref"},{"subj":"C15","pred":"source","obj":"genia-medco-coref"},{"subj":"C16","pred":"source","obj":"genia-medco-coref"},{"subj":"C17","pred":"source","obj":"genia-medco-coref"}]},{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":15,"end":32},"obj":"protein"},{"id":"T2","span":{"begin":37,"end":51},"obj":"protein"},{"id":"T3","span":{"begin":66,"end":71},"obj":"protein"},{"id":"T4","span":{"begin":89,"end":104},"obj":"cell_type"},{"id":"T5","span":{"begin":108,"end":129},"obj":"protein"},{"id":"T6","span":{"begin":201,"end":217},"obj":"protein"},{"id":"T7","span":{"begin":219,"end":228},"obj":"protein"},{"id":"T8","span":{"begin":235,"end":255},"obj":"protein"},{"id":"T9","span":{"begin":259,"end":291},"obj":"protein"},{"id":"T10","span":{"begin":304,"end":318},"obj":"protein"},{"id":"T11","span":{"begin":320,"end":325},"obj":"protein"},{"id":"T12","span":{"begin":341,"end":356},"obj":"cell_type"},{"id":"T13","span":{"begin":454,"end":458},"obj":"protein"},{"id":"T14","span":{"begin":467,"end":472},"obj":"protein"},{"id":"T15","span":{"begin":562,"end":571},"obj":"protein"},{"id":"T16","span":{"begin":582,"end":586},"obj":"protein"},{"id":"T17","span":{"begin":616,"end":625},"obj":"cell_type"},{"id":"T18","span":{"begin":680,"end":697},"obj":"protein"},{"id":"T19","span":{"begin":728,"end":732},"obj":"protein"},{"id":"T20","span":{"begin":741,"end":746},"obj":"protein"},{"id":"T21","span":{"begin":761,"end":770},"obj":"cell_type"},{"id":"T22","span":{"begin":785,"end":789},"obj":"protein"},{"id":"T23","span":{"begin":810,"end":819},"obj":"protein"},{"id":"T24","span":{"begin":854,"end":863},"obj":"protein"},{"id":"T25","span":{"begin":877,"end":904},"obj":"DNA"},{"id":"T26","span":{"begin":914,"end":928},"obj":"DNA"},{"id":"T27","span":{"begin":947,"end":951},"obj":"protein"},{"id":"T28","span":{"begin":1048,"end":1062},"obj":"protein"},{"id":"T29","span":{"begin":1080,"end":1090},"obj":"protein"},{"id":"T30","span":{"begin":1092,"end":1107},"obj":"protein"},{"id":"T31","span":{"begin":1133,"end":1138},"obj":"protein"},{"id":"T32","span":{"begin":1153,"end":1157},"obj":"protein"},{"id":"T33","span":{"begin":1161,"end":1170},"obj":"cell_type"},{"id":"T34","span":{"begin":1196,"end":1225},"obj":"protein"},{"id":"T35","span":{"begin":1227,"end":1238},"obj":"protein"},{"id":"T36","span":{"begin":1291,"end":1295},"obj":"protein"},{"id":"T37","span":{"begin":1336,"end":1340},"obj":"protein"},{"id":"T38","span":{"begin":1349,"end":1354},"obj":"protein"},{"id":"T39","span":{"begin":1372,"end":1381},"obj":"cell_type"},{"id":"T40","span":{"begin":1398,"end":1407},"obj":"protein"},{"id":"T41","span":{"begin":1409,"end":1413},"obj":"protein"},{"id":"T42","span":{"begin":1418,"end":1429},"obj":"protein"},{"id":"T43","span":{"begin":1487,"end":1504},"obj":"protein"},{"id":"T44","span":{"begin":1509,"end":1548},"obj":"protein"},{"id":"T45","span":{"begin":1559,"end":1573},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"jnlpba-st-training"},{"subj":"T2","pred":"source","obj":"jnlpba-st-training"},{"subj":"T3","pred":"source","obj":"jnlpba-st-training"},{"subj":"T4","pred":"source","obj":"jnlpba-st-training"},{"subj":"T5","pred":"source","obj":"jnlpba-st-training"},{"subj":"T6","pred":"source","obj":"jnlpba-st-training"},{"subj":"T7","pred":"source","obj":"jnlpba-st-training"},{"subj":"T8","pred":"source","obj":"jnlpba-st-training"},{"subj":"T9","pred":"source","obj":"jnlpba-st-training"},{"subj":"T10","pred":"source","obj":"jnlpba-st-training"},{"subj":"T11","pred":"source","obj":"jnlpba-st-training"},{"subj":"T12","pred":"source","obj":"jnlpba-st-training"},{"subj":"T13","pred":"source","obj":"jnlpba-st-training"},{"subj":"T14","pred":"source","obj":"jnlpba-st-training"},{"subj":"T15","pred":"source","obj":"jnlpba-st-training"},{"subj":"T16","pred":"source","obj":"jnlpba-st-training"},{"subj":"T17","pred":"source","obj":"jnlpba-st-training"},{"subj":"T18","pred":"source","obj":"jnlpba-st-training"},{"subj":"T19","pred":"source","obj":"jnlpba-st-training"},{"subj":"T20","pred":"source","obj":"jnlpba-st-training"},{"subj":"T21","pred":"source","obj":"jnlpba-st-training"},{"subj":"T22","pred":"source","obj":"jnlpba-st-training"},{"subj":"T23","pred":"source","obj":"jnlpba-st-training"},{"subj":"T24","pred":"source","obj":"jnlpba-st-training"},{"subj":"T25","pred":"source","obj":"jnlpba-st-training"},{"subj":"T26","pred":"source","obj":"jnlpba-st-training"},{"subj":"T27","pred":"source","obj":"jnlpba-st-training"},{"subj":"T28","pred":"source","obj":"jnlpba-st-training"},{"subj":"T29","pred":"source","obj":"jnlpba-st-training"},{"subj":"T30","pred":"source","obj":"jnlpba-st-training"},{"subj":"T31","pred":"source","obj":"jnlpba-st-training"},{"subj":"T32","pred":"source","obj":"jnlpba-st-training"},{"subj":"T33","pred":"source","obj":"jnlpba-st-training"},{"subj":"T34","pred":"source","obj":"jnlpba-st-training"},{"subj":"T35","pred":"source","obj":"jnlpba-st-training"},{"subj":"T36","pred":"source","obj":"jnlpba-st-training"},{"subj":"T37","pred":"source","obj":"jnlpba-st-training"},{"subj":"T38","pred":"source","obj":"jnlpba-st-training"},{"subj":"T39","pred":"source","obj":"jnlpba-st-training"},{"subj":"T40","pred":"source","obj":"jnlpba-st-training"},{"subj":"T41","pred":"source","obj":"jnlpba-st-training"},{"subj":"T42","pred":"source","obj":"jnlpba-st-training"},{"subj":"T43","pred":"source","obj":"jnlpba-st-training"},{"subj":"T44","pred":"source","obj":"jnlpba-st-training"},{"subj":"T45","pred":"source","obj":"jnlpba-st-training"}]},{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":169},"obj":"Sentence"},{"id":"S2","span":{"begin":170,"end":357},"obj":"Sentence"},{"id":"S3","span":{"begin":358,"end":418},"obj":"Sentence"},{"id":"S4","span":{"begin":419,"end":581},"obj":"Sentence"},{"id":"S5","span":{"begin":582,"end":771},"obj":"Sentence"},{"id":"S6","span":{"begin":772,"end":952},"obj":"Sentence"},{"id":"S7","span":{"begin":953,"end":1024},"obj":"Sentence"},{"id":"S8","span":{"begin":1025,"end":1324},"obj":"Sentence"},{"id":"S9","span":{"begin":1325,"end":1585},"obj":"Sentence"}],"attributes":[{"subj":"S1","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S2","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S3","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S4","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S5","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S6","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S7","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S8","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S9","pred":"source","obj":"pubmed-sentences-benchmark"}]},{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":15,"end":32},"obj":"protein_complex"},{"id":"T2","span":{"begin":37,"end":51},"obj":"protein_molecule"},{"id":"T3","span":{"begin":66,"end":71},"obj":"protein_molecule"},{"id":"T4","span":{"begin":89,"end":104},"obj":"cell_type"},{"id":"T5","span":{"begin":108,"end":129},"obj":"protein_molecule"},{"id":"T6","span":{"begin":133,"end":168},"obj":"virus"},{"id":"T7","span":{"begin":201,"end":217},"obj":"protein_molecule"},{"id":"T8","span":{"begin":219,"end":228},"obj":"protein_molecule"},{"id":"T9","span":{"begin":235,"end":255},"obj":"protein_domain_or_region"},{"id":"T10","span":{"begin":259,"end":291},"obj":"protein_family_or_group"},{"id":"T11","span":{"begin":304,"end":318},"obj":"protein_molecule"},{"id":"T12","span":{"begin":320,"end":325},"obj":"protein_molecule"},{"id":"T13","span":{"begin":341,"end":356},"obj":"cell_type"},{"id":"T14","span":{"begin":454,"end":458},"obj":"protein_molecule"},{"id":"T15","span":{"begin":467,"end":472},"obj":"protein_molecule"},{"id":"T16","span":{"begin":553,"end":557},"obj":"nucleotide"},{"id":"T17","span":{"begin":562,"end":571},"obj":"protein_complex"},{"id":"T18","span":{"begin":582,"end":586},"obj":"protein_molecule"},{"id":"T19","span":{"begin":595,"end":599},"obj":"nucleotide"},{"id":"T20","span":{"begin":616,"end":625},"obj":"cell_type"},{"id":"T21","span":{"begin":680,"end":697},"obj":"protein_complex"},{"id":"T22","span":{"begin":708,"end":716},"obj":"other_organic_compound"},{"id":"T23","span":{"begin":728,"end":732},"obj":"protein_molecule"},{"id":"T24","span":{"begin":741,"end":746},"obj":"protein_molecule"},{"id":"T25","span":{"begin":761,"end":770},"obj":"cell_type"},{"id":"T26","span":{"begin":785,"end":789},"obj":"protein_molecule"},{"id":"T27","span":{"begin":810,"end":819},"obj":"protein_complex"},{"id":"T28","span":{"begin":854,"end":863},"obj":"protein_complex"},{"id":"T29","span":{"begin":877,"end":881},"obj":"DNA_domain_or_region"},{"id":"T30","span":{"begin":882,"end":891},"obj":"protein_complex"},{"id":"T31","span":{"begin":914,"end":919},"obj":"protein_molecule"},{"id":"T32","span":{"begin":947,"end":951},"obj":"protein_molecule"},{"id":"T33","span":{"begin":995,"end":1023},"obj":"other_name"},{"id":"T34","span":{"begin":1048,"end":1062},"obj":"protein_molecule"},{"id":"T35","span":{"begin":1064,"end":1073},"obj":"other_organic_compound"},{"id":"T36","span":{"begin":1080,"end":1090},"obj":"protein_molecule"},{"id":"T37","span":{"begin":1092,"end":1107},"obj":"protein_molecule"},{"id":"T38","span":{"begin":1133,"end":1138},"obj":"protein_molecule"},{"id":"T39","span":{"begin":1153,"end":1157},"obj":"protein_molecule"},{"id":"T40","span":{"begin":1161,"end":1170},"obj":"cell_type"},{"id":"T41","span":{"begin":1196,"end":1225},"obj":"protein_molecule"},{"id":"T42","span":{"begin":1227,"end":1238},"obj":"protein_molecule"},{"id":"T43","span":{"begin":1241,"end":1251},"obj":"other_organic_compound"},{"id":"T44","span":{"begin":1257,"end":1291},"obj":"other_name"},{"id":"T45","span":{"begin":1291,"end":1295},"obj":"protein_family_or_group"},{"id":"T46","span":{"begin":1306,"end":1314},"obj":"other_organic_compound"},{"id":"T47","span":{"begin":1330,"end":1335},"obj":"virus"},{"id":"T48","span":{"begin":1336,"end":1340},"obj":"protein_molecule"},{"id":"T49","span":{"begin":1349,"end":1354},"obj":"protein_molecule"},{"id":"T50","span":{"begin":1372,"end":1381},"obj":"cell_type"},{"id":"T51","span":{"begin":1398,"end":1407},"obj":"protein_complex"},{"id":"T52","span":{"begin":1409,"end":1413},"obj":"protein_family_or_group"},{"id":"T53","span":{"begin":1418,"end":1429},"obj":"protein_molecule"},{"id":"T54","span":{"begin":1487,"end":1504},"obj":"protein_complex"},{"id":"T55","span":{"begin":1509,"end":1548},"obj":"protein_molecule"},{"id":"T56","span":{"begin":1559,"end":1573},"obj":"protein_molecule"}],"attributes":[{"subj":"T1","pred":"source","obj":"GENIAcorpus"},{"subj":"T2","pred":"source","obj":"GENIAcorpus"},{"subj":"T3","pred":"source","obj":"GENIAcorpus"},{"subj":"T4","pred":"source","obj":"GENIAcorpus"},{"subj":"T5","pred":"source","obj":"GENIAcorpus"},{"subj":"T6","pred":"source","obj":"GENIAcorpus"},{"subj":"T7","pred":"source","obj":"GENIAcorpus"},{"subj":"T8","pred":"source","obj":"GENIAcorpus"},{"subj":"T9","pred":"source","obj":"GENIAcorpus"},{"subj":"T10","pred":"source","obj":"GENIAcorpus"},{"subj":"T11","pred":"source","obj":"GENIAcorpus"},{"subj":"T12","pred":"source","obj":"GENIAcorpus"},{"subj":"T13","pred":"source","obj":"GENIAcorpus"},{"subj":"T14","pred":"source","obj":"GENIAcorpus"},{"subj":"T15","pred":"source","obj":"GENIAcorpus"},{"subj":"T16","pred":"source","obj":"GENIAcorpus"},{"subj":"T17","pred":"source","obj":"GENIAcorpus"},{"subj":"T18","pred":"source","obj":"GENIAcorpus"},{"subj":"T19","pred":"source","obj":"GENIAcorpus"},{"subj":"T20","pred":"source","obj":"GENIAcorpus"},{"subj":"T21","pred":"source","obj":"GENIAcorpus"},{"subj":"T22","pred":"source","obj":"GENIAcorpus"},{"subj":"T23","pred":"source","obj":"GENIAcorpus"},{"subj":"T24","pred":"source","obj":"GENIAcorpus"},{"subj":"T25","pred":"source","obj":"GENIAcorpus"},{"subj":"T26","pred":"source","obj":"GENIAcorpus"},{"subj":"T27","pred":"source","obj":"GENIAcorpus"},{"subj":"T28","pred":"source","obj":"GENIAcorpus"},{"subj":"T29","pred":"source","obj":"GENIAcorpus"},{"subj":"T30","pred":"source","obj":"GENIAcorpus"},{"subj":"T31","pred":"source","obj":"GENIAcorpus"},{"subj":"T32","pred":"source","obj":"GENIAcorpus"},{"subj":"T33","pred":"source","obj":"GENIAcorpus"},{"subj":"T34","pred":"source","obj":"GENIAcorpus"},{"subj":"T35","pred":"source","obj":"GENIAcorpus"},{"subj":"T36","pred":"source","obj":"GENIAcorpus"},{"subj":"T37","pred":"source","obj":"GENIAcorpus"},{"subj":"T38","pred":"source","obj":"GENIAcorpus"},{"subj":"T39","pred":"source","obj":"GENIAcorpus"},{"subj":"T40","pred":"source","obj":"GENIAcorpus"},{"subj":"T41","pred":"source","obj":"GENIAcorpus"},{"subj":"T42","pred":"source","obj":"GENIAcorpus"},{"subj":"T43","pred":"source","obj":"GENIAcorpus"},{"subj":"T44","pred":"source","obj":"GENIAcorpus"},{"subj":"T45","pred":"source","obj":"GENIAcorpus"},{"subj":"T46","pred":"source","obj":"GENIAcorpus"},{"subj":"T47","pred":"source","obj":"GENIAcorpus"},{"subj":"T48","pred":"source","obj":"GENIAcorpus"},{"subj":"T49","pred":"source","obj":"GENIAcorpus"},{"subj":"T50","pred":"source","obj":"GENIAcorpus"},{"subj":"T51","pred":"source","obj":"GENIAcorpus"},{"subj":"T52","pred":"source","obj":"GENIAcorpus"},{"subj":"T53","pred":"source","obj":"GENIAcorpus"},{"subj":"T54","pred":"source","obj":"GENIAcorpus"},{"subj":"T55","pred":"source","obj":"GENIAcorpus"},{"subj":"T56","pred":"source","obj":"GENIAcorpus"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"genia-medco-coref","color":"#939cec","default":true},{"id":"jnlpba-st-training","color":"#b6ec93"},{"id":"pubmed-sentences-benchmark","color":"#ec93d0"},{"id":"GENIAcorpus","color":"#93eaec"}]}]}}