PubMed:10080875
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10080875","sourcedb":"PubMed","sourceid":"10080875","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10080875","text":"Involvement of NF-kappaB p50/p65 heterodimer in activation of the human pro-interleukin-1beta gene at two subregions of the upstream enhancer element.\nA region between-3134 and -2729 bp upstream from the transcription site of the human pro-interleukin 1beta (proIL-1beta) gene was identified as an LPS-responsive enhancer element. In this study, the influence of the sequences located between -3134 and -2987 on the transcriptional activity of the proIL-1beta gene in LPS-stimulated Raw 264.7 cells was examined in detail. The results obtained by transient transfection of fos -CAT constructs that contained serial 5'-deletion mutations showed that the region between -3134 and -3059 appears to be required for the induction of transcription by LPS. Gel shift assay studies with synthetic oligonucleotides corresponding to partial sequences of the latter region and nuclear extracts from stimulated cells revealed specific protein binding sites between -3110 and -3090 and between -3079 and -3059. These specific bindings were time and LPS dose dependent. The results of supershift analysis using specific antibodies against transcription factors suggested that both binding complexes contained the NF-kappaB components p50 and p65, and did not contain other NF-kappaB proteins (p52, c-Rel, Rel B), AP-1 proteins (c-Fos, C-Jun), CREB or C/EBPbeta (NF-IL6). Mutation of either of the putative NF-kappaB-binding sites in the enhancer element decreased the LPS-stimulated transcriptional activity. These data indicated that two NF-kappaB-binding sites, which are located between -3134 and -3059, are critical for the activation of proIL-1beta gene transcription.","tracks":[{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":62,"end":98},"obj":"NP"},{"id":"C2","span":{"begin":226,"end":276},"obj":"NP"},{"id":"C3","span":{"begin":444,"end":464},"obj":"NP"},{"id":"C4","span":{"begin":547,"end":592},"obj":"NP"},{"id":"C5","span":{"begin":593,"end":597},"obj":"NP"},{"id":"C7","span":{"begin":668,"end":683},"obj":"NP"},{"id":"C6","span":{"begin":649,"end":683},"obj":"NP"},{"id":"C8","span":{"begin":844,"end":861},"obj":"NP"},{"id":"C9","span":{"begin":1521,"end":1548},"obj":"NP"},{"id":"C10","span":{"begin":1550,"end":1555},"obj":"NP"},{"id":"C11","span":{"begin":1576,"end":1591},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C2","obj":"C1"},{"id":"R2","pred":"coref-ident","subj":"C3","obj":"C2"},{"id":"R3","pred":"coref-relat","subj":"C5","obj":"C4"},{"id":"R4","pred":"coref-ident","subj":"C8","obj":"C6"},{"id":"R5","pred":"coref-relat","subj":"C10","obj":"C9"},{"id":"R6","pred":"coref-ident","subj":"C11","obj":"C7"}],"attributes":[{"subj":"C1","pred":"source","obj":"genia-medco-coref"},{"subj":"C2","pred":"source","obj":"genia-medco-coref"},{"subj":"C3","pred":"source","obj":"genia-medco-coref"},{"subj":"C4","pred":"source","obj":"genia-medco-coref"},{"subj":"C5","pred":"source","obj":"genia-medco-coref"},{"subj":"C7","pred":"source","obj":"genia-medco-coref"},{"subj":"C6","pred":"source","obj":"genia-medco-coref"},{"subj":"C8","pred":"source","obj":"genia-medco-coref"},{"subj":"C9","pred":"source","obj":"genia-medco-coref"},{"subj":"C10","pred":"source","obj":"genia-medco-coref"},{"subj":"C11","pred":"source","obj":"genia-medco-coref"}]},{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":15,"end":44},"obj":"protein"},{"id":"T2","span":{"begin":66,"end":98},"obj":"DNA"},{"id":"T3","span":{"begin":124,"end":149},"obj":"DNA"},{"id":"T4","span":{"begin":204,"end":222},"obj":"DNA"},{"id":"T5","span":{"begin":230,"end":276},"obj":"DNA"},{"id":"T6","span":{"begin":298,"end":329},"obj":"DNA"},{"id":"T7","span":{"begin":385,"end":408},"obj":"DNA"},{"id":"T8","span":{"begin":448,"end":464},"obj":"DNA"},{"id":"T9","span":{"begin":468,"end":498},"obj":"cell_line"},{"id":"T10","span":{"begin":577,"end":592},"obj":"DNA"},{"id":"T11","span":{"begin":608,"end":636},"obj":"DNA"},{"id":"T12","span":{"begin":779,"end":805},"obj":"DNA"},{"id":"T13","span":{"begin":923,"end":944},"obj":"DNA"},{"id":"T14","span":{"begin":1125,"end":1146},"obj":"protein"},{"id":"T15","span":{"begin":1199,"end":1219},"obj":"protein"},{"id":"T16","span":{"begin":1220,"end":1223},"obj":"protein"},{"id":"T17","span":{"begin":1228,"end":1231},"obj":"protein"},{"id":"T18","span":{"begin":1259,"end":1277},"obj":"protein"},{"id":"T19","span":{"begin":1279,"end":1282},"obj":"protein"},{"id":"T20","span":{"begin":1284,"end":1289},"obj":"protein"},{"id":"T21","span":{"begin":1291,"end":1296},"obj":"protein"},{"id":"T22","span":{"begin":1299,"end":1312},"obj":"protein"},{"id":"T23","span":{"begin":1314,"end":1319},"obj":"protein"},{"id":"T24","span":{"begin":1321,"end":1326},"obj":"protein"},{"id":"T25","span":{"begin":1329,"end":1333},"obj":"protein"},{"id":"T26","span":{"begin":1337,"end":1346},"obj":"protein"},{"id":"T27","span":{"begin":1348,"end":1354},"obj":"protein"},{"id":"T28","span":{"begin":1392,"end":1415},"obj":"DNA"},{"id":"T29","span":{"begin":1525,"end":1548},"obj":"DNA"},{"id":"T30","span":{"begin":1576,"end":1591},"obj":"DNA"},{"id":"T31","span":{"begin":1628,"end":1644},"obj":"DNA"}],"attributes":[{"subj":"T1","pred":"source","obj":"jnlpba-st-training"},{"subj":"T2","pred":"source","obj":"jnlpba-st-training"},{"subj":"T3","pred":"source","obj":"jnlpba-st-training"},{"subj":"T4","pred":"source","obj":"jnlpba-st-training"},{"subj":"T5","pred":"source","obj":"jnlpba-st-training"},{"subj":"T6","pred":"source","obj":"jnlpba-st-training"},{"subj":"T7","pred":"source","obj":"jnlpba-st-training"},{"subj":"T8","pred":"source","obj":"jnlpba-st-training"},{"subj":"T9","pred":"source","obj":"jnlpba-st-training"},{"subj":"T10","pred":"source","obj":"jnlpba-st-training"},{"subj":"T11","pred":"source","obj":"jnlpba-st-training"},{"subj":"T12","pred":"source","obj":"jnlpba-st-training"},{"subj":"T13","pred":"source","obj":"jnlpba-st-training"},{"subj":"T14","pred":"source","obj":"jnlpba-st-training"},{"subj":"T15","pred":"source","obj":"jnlpba-st-training"},{"subj":"T16","pred":"source","obj":"jnlpba-st-training"},{"subj":"T17","pred":"source","obj":"jnlpba-st-training"},{"subj":"T18","pred":"source","obj":"jnlpba-st-training"},{"subj":"T19","pred":"source","obj":"jnlpba-st-training"},{"subj":"T20","pred":"source","obj":"jnlpba-st-training"},{"subj":"T21","pred":"source","obj":"jnlpba-st-training"},{"subj":"T22","pred":"source","obj":"jnlpba-st-training"},{"subj":"T23","pred":"source","obj":"jnlpba-st-training"},{"subj":"T24","pred":"source","obj":"jnlpba-st-training"},{"subj":"T25","pred":"source","obj":"jnlpba-st-training"},{"subj":"T26","pred":"source","obj":"jnlpba-st-training"},{"subj":"T27","pred":"source","obj":"jnlpba-st-training"},{"subj":"T28","pred":"source","obj":"jnlpba-st-training"},{"subj":"T29","pred":"source","obj":"jnlpba-st-training"},{"subj":"T30","pred":"source","obj":"jnlpba-st-training"},{"subj":"T31","pred":"source","obj":"jnlpba-st-training"}]},{"project":"FSU-PRGE","denotations":[{"id":"T1","span":{"begin":15,"end":32},"obj":"protein"},{"id":"T2","span":{"begin":72,"end":93},"obj":"protein"},{"id":"T3","span":{"begin":240,"end":257},"obj":"protein"},{"id":"T4","span":{"begin":259,"end":270},"obj":"protein"},{"id":"T5","span":{"begin":448,"end":459},"obj":"protein"},{"id":"T6","span":{"begin":573,"end":576},"obj":"protein"},{"id":"T7","span":{"begin":578,"end":581},"obj":"protein"},{"id":"T8","span":{"begin":1125,"end":1146},"obj":"protein"},{"id":"T9","span":{"begin":1199,"end":1208},"obj":"protein"},{"id":"T10","span":{"begin":1220,"end":1223},"obj":"protein"},{"id":"T11","span":{"begin":1228,"end":1231},"obj":"protein"},{"id":"T12","span":{"begin":1259,"end":1268},"obj":"protein"},{"id":"T13","span":{"begin":1279,"end":1282},"obj":"protein"},{"id":"T14","span":{"begin":1284,"end":1289},"obj":"protein"},{"id":"T15","span":{"begin":1291,"end":1296},"obj":"protein"},{"id":"T16","span":{"begin":1299,"end":1303},"obj":"protein"},{"id":"T17","span":{"begin":1314,"end":1319},"obj":"protein"},{"id":"T18","span":{"begin":1321,"end":1326},"obj":"protein"},{"id":"T19","span":{"begin":1329,"end":1333},"obj":"protein"},{"id":"T20","span":{"begin":1337,"end":1346},"obj":"protein"},{"id":"T21","span":{"begin":1348,"end":1354},"obj":"protein"},{"id":"T22","span":{"begin":1392,"end":1401},"obj":"protein"},{"id":"T23","span":{"begin":1525,"end":1534},"obj":"protein"},{"id":"T24","span":{"begin":1628,"end":1639},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"FSU-PRGE"},{"subj":"T2","pred":"source","obj":"FSU-PRGE"},{"subj":"T3","pred":"source","obj":"FSU-PRGE"},{"subj":"T4","pred":"source","obj":"FSU-PRGE"},{"subj":"T5","pred":"source","obj":"FSU-PRGE"},{"subj":"T6","pred":"source","obj":"FSU-PRGE"},{"subj":"T7","pred":"source","obj":"FSU-PRGE"},{"subj":"T8","pred":"source","obj":"FSU-PRGE"},{"subj":"T9","pred":"source","obj":"FSU-PRGE"},{"subj":"T10","pred":"source","obj":"FSU-PRGE"},{"subj":"T11","pred":"source","obj":"FSU-PRGE"},{"subj":"T12","pred":"source","obj":"FSU-PRGE"},{"subj":"T13","pred":"source","obj":"FSU-PRGE"},{"subj":"T14","pred":"source","obj":"FSU-PRGE"},{"subj":"T15","pred":"source","obj":"FSU-PRGE"},{"subj":"T16","pred":"source","obj":"FSU-PRGE"},{"subj":"T17","pred":"source","obj":"FSU-PRGE"},{"subj":"T18","pred":"source","obj":"FSU-PRGE"},{"subj":"T19","pred":"source","obj":"FSU-PRGE"},{"subj":"T20","pred":"source","obj":"FSU-PRGE"},{"subj":"T21","pred":"source","obj":"FSU-PRGE"},{"subj":"T22","pred":"source","obj":"FSU-PRGE"},{"subj":"T23","pred":"source","obj":"FSU-PRGE"},{"subj":"T24","pred":"source","obj":"FSU-PRGE"}]},{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":150},"obj":"Sentence"},{"id":"S2","span":{"begin":151,"end":330},"obj":"Sentence"},{"id":"S3","span":{"begin":331,"end":522},"obj":"Sentence"},{"id":"S4","span":{"begin":523,"end":749},"obj":"Sentence"},{"id":"S5","span":{"begin":750,"end":997},"obj":"Sentence"},{"id":"S6","span":{"begin":998,"end":1055},"obj":"Sentence"},{"id":"S7","span":{"begin":1056,"end":1356},"obj":"Sentence"},{"id":"S8","span":{"begin":1357,"end":1494},"obj":"Sentence"},{"id":"S9","span":{"begin":1495,"end":1659},"obj":"Sentence"}],"attributes":[{"subj":"S1","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S2","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S3","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S4","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S5","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S6","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S7","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S8","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S9","pred":"source","obj":"pubmed-sentences-benchmark"}]},{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":15,"end":24},"obj":"protein_complex"},{"id":"T2","span":{"begin":66,"end":98},"obj":"DNA_domain_or_region"},{"id":"T3","span":{"begin":124,"end":149},"obj":"DNA_domain_or_region"},{"id":"T4","span":{"begin":204,"end":222},"obj":"DNA_domain_or_region"},{"id":"T5","span":{"begin":230,"end":257},"obj":"protein_molecule"},{"id":"T6","span":{"begin":259,"end":270},"obj":"protein_molecule"},{"id":"T7","span":{"begin":298,"end":329},"obj":"DNA_domain_or_region"},{"id":"T8","span":{"begin":385,"end":408},"obj":"DNA_domain_or_region"},{"id":"T9","span":{"begin":416,"end":440},"obj":"other_name"},{"id":"T10","span":{"begin":448,"end":464},"obj":"DNA_domain_or_region"},{"id":"T11","span":{"begin":468,"end":498},"obj":"cell_line"},{"id":"T12","span":{"begin":577,"end":592},"obj":"DNA_family_or_group"},{"id":"T13","span":{"begin":608,"end":636},"obj":"DNA_domain_or_region"},{"id":"T14","span":{"begin":745,"end":748},"obj":"lipid"},{"id":"T15","span":{"begin":779,"end":805},"obj":"DNA_family_or_group"},{"id":"T16","span":{"begin":866,"end":882},"obj":"cell_component"},{"id":"T17","span":{"begin":923,"end":944},"obj":"DNA_domain_or_region"},{"id":"T18","span":{"begin":1036,"end":1039},"obj":"lipid"},{"id":"T19","span":{"begin":1125,"end":1146},"obj":"protein_family_or_group"},{"id":"T20","span":{"begin":1199,"end":1208},"obj":"protein_complex"},{"id":"T21","span":{"begin":1220,"end":1223},"obj":"protein_subunit"},{"id":"T22","span":{"begin":1228,"end":1231},"obj":"protein_subunit"},{"id":"T23","span":{"begin":1259,"end":1268},"obj":"protein_complex"},{"id":"T24","span":{"begin":1279,"end":1282},"obj":"protein_subunit"},{"id":"T25","span":{"begin":1284,"end":1289},"obj":"protein_subunit"},{"id":"T26","span":{"begin":1291,"end":1296},"obj":"protein_subunit"},{"id":"T27","span":{"begin":1299,"end":1312},"obj":"protein_family_or_group"},{"id":"T28","span":{"begin":1314,"end":1319},"obj":"protein_molecule"},{"id":"T29","span":{"begin":1321,"end":1326},"obj":"protein_molecule"},{"id":"T30","span":{"begin":1329,"end":1333},"obj":"protein_family_or_group"},{"id":"T31","span":{"begin":1337,"end":1346},"obj":"protein_molecule"},{"id":"T32","span":{"begin":1348,"end":1354},"obj":"protein_molecule"},{"id":"T33","span":{"begin":1392,"end":1415},"obj":"DNA_domain_or_region"},{"id":"T34","span":{"begin":1454,"end":1457},"obj":"lipid"},{"id":"T35","span":{"begin":1469,"end":1493},"obj":"other_name"},{"id":"T36","span":{"begin":1525,"end":1548},"obj":"DNA_domain_or_region"},{"id":"T37","span":{"begin":1576,"end":1591},"obj":"DNA_domain_or_region"},{"id":"T38","span":{"begin":1628,"end":1644},"obj":"DNA_domain_or_region"}],"attributes":[{"subj":"T1","pred":"source","obj":"GENIAcorpus"},{"subj":"T2","pred":"source","obj":"GENIAcorpus"},{"subj":"T3","pred":"source","obj":"GENIAcorpus"},{"subj":"T4","pred":"source","obj":"GENIAcorpus"},{"subj":"T5","pred":"source","obj":"GENIAcorpus"},{"subj":"T6","pred":"source","obj":"GENIAcorpus"},{"subj":"T7","pred":"source","obj":"GENIAcorpus"},{"subj":"T8","pred":"source","obj":"GENIAcorpus"},{"subj":"T9","pred":"source","obj":"GENIAcorpus"},{"subj":"T10","pred":"source","obj":"GENIAcorpus"},{"subj":"T11","pred":"source","obj":"GENIAcorpus"},{"subj":"T12","pred":"source","obj":"GENIAcorpus"},{"subj":"T13","pred":"source","obj":"GENIAcorpus"},{"subj":"T14","pred":"source","obj":"GENIAcorpus"},{"subj":"T15","pred":"source","obj":"GENIAcorpus"},{"subj":"T16","pred":"source","obj":"GENIAcorpus"},{"subj":"T17","pred":"source","obj":"GENIAcorpus"},{"subj":"T18","pred":"source","obj":"GENIAcorpus"},{"subj":"T19","pred":"source","obj":"GENIAcorpus"},{"subj":"T20","pred":"source","obj":"GENIAcorpus"},{"subj":"T21","pred":"source","obj":"GENIAcorpus"},{"subj":"T22","pred":"source","obj":"GENIAcorpus"},{"subj":"T23","pred":"source","obj":"GENIAcorpus"},{"subj":"T24","pred":"source","obj":"GENIAcorpus"},{"subj":"T25","pred":"source","obj":"GENIAcorpus"},{"subj":"T26","pred":"source","obj":"GENIAcorpus"},{"subj":"T27","pred":"source","obj":"GENIAcorpus"},{"subj":"T28","pred":"source","obj":"GENIAcorpus"},{"subj":"T29","pred":"source","obj":"GENIAcorpus"},{"subj":"T30","pred":"source","obj":"GENIAcorpus"},{"subj":"T31","pred":"source","obj":"GENIAcorpus"},{"subj":"T32","pred":"source","obj":"GENIAcorpus"},{"subj":"T33","pred":"source","obj":"GENIAcorpus"},{"subj":"T34","pred":"source","obj":"GENIAcorpus"},{"subj":"T35","pred":"source","obj":"GENIAcorpus"},{"subj":"T36","pred":"source","obj":"GENIAcorpus"},{"subj":"T37","pred":"source","obj":"GENIAcorpus"},{"subj":"T38","pred":"source","obj":"GENIAcorpus"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"genia-medco-coref","color":"#93ecea","default":true},{"id":"jnlpba-st-training","color":"#ecd093"},{"id":"FSU-PRGE","color":"#b693ec"},{"id":"pubmed-sentences-benchmark","color":"#93ec9c"},{"id":"GENIAcorpus","color":"#ec93a3"}]}]}}