PubMed:10026210
Annnotations
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-104 | Sentence | denotes | A novel PDZ domain containing guanine nucleotide exchange factor links heterotrimeric G proteins to Rho. |
| T2 | 105-194 | Sentence | denotes | Small GTP-binding proteins of the Rho family play a critical role in signal transduction. |
| T3 | 195-296 | Sentence | denotes | However, there is still very limited information on how they are activated by cell surface receptors. |
| T4 | 297-562 | Sentence | denotes | Here, we used a consensus sequence for Dbl domains of Rho guanine nucleotide exchange factors (GEFs) to search DNA data bases, and identified a novel human GEF for Rho-related GTPases harboring structural features indicative of its possible regulatory mechanism(s). |
| T5 | 563-802 | Sentence | denotes | This protein contained a tandem DH/PH domain closely related to those of Rho-specific GEFs, a PDZ domain, a proline-rich domain, and an area of homology to Lsc, p115-RhoGEF, and a Drosophila RhoGEF that was termed Lsc-homology (LH) domain. |
| T6 | 803-980 | Sentence | denotes | This novel molecule, designated PDZ-RhoGEF, activated biological and biochemical pathways specific for Rho, and activation of these pathways required an intact DH and PH domain. |
| T7 | 981-1150 | Sentence | denotes | However, the PDZ domain was dispensable for these functions, and mutants lacking the LH domain were more active, suggesting a negative regulatory role for the LH domain. |
| T8 | 1151-1348 | Sentence | denotes | A search for additional molecules exhibiting an LH domain revealed a limited homology with the catalytic region of a newly identified GTPase-activating protein for heterotrimeric G proteins, RGS14. |
| T9 | 1349-1468 | Sentence | denotes | This prompted us to investigate whether PDZ-RhoGEF could interact with representative members of each G protein family. |
| T10 | 1469-1658 | Sentence | denotes | We found that PDZ-RhoGEF was able to form, in vivo, stable complexes with two members of the Galpha12 family, Galpha12 and Galpha13, and that this interaction was mediated by the LH domain. |
| T11 | 1659-1776 | Sentence | denotes | Furthermore, we obtained evidence to suggest that PDZ-RhoGEF mediates the activation of Rho by Galpha12 and Galpha13. |
| T12 | 1777-2105 | Sentence | denotes | Together, these findings suggest the existence of a novel mechanism whereby the large family of cell surface receptors that transmit signals through heterotrimeric G proteins activate Rho-dependent pathways: by stimulating the activity of members of the Galpha12 family which, in turn, activate an exchange factor acting on Rho. |
FSU-PRGE
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 30-64 | protein | denotes | guanine nucleotide exchange factor |
| T2 | 71-96 | protein | denotes | heterotrimeric G proteins |
| T3 | 100-103 | protein | denotes | Rho |
| T4 | 105-149 | protein | denotes | Small GTP-binding proteins of the Rho family |
| T5 | 336-339 | protein | denotes | Dbl |
| T6 | 351-390 | protein | denotes | Rho guanine nucleotide exchange factors |
| T7 | 392-396 | protein | denotes | GEFs |
| T8 | 453-456 | protein | denotes | GEF |
| T9 | 461-464 | protein | denotes | Rho |
| T10 | 473-480 | protein | denotes | GTPases |
| T11 | 636-653 | protein | denotes | Rho-specific GEFs |
| T12 | 719-722 | protein | denotes | Lsc |
| T13 | 724-735 | protein | denotes | p115-RhoGEF |
| T14 | 754-760 | protein | denotes | RhoGEF |
| T15 | 777-780 | protein | denotes | Lsc |
| T16 | 835-845 | protein | denotes | PDZ-RhoGEF |
| T17 | 906-909 | protein | denotes | Rho |
| T18 | 1285-1310 | protein | denotes | GTPase-activating protein |
| T19 | 1315-1340 | protein | denotes | heterotrimeric G proteins |
| T20 | 1342-1347 | protein | denotes | RGS14 |
| T21 | 1389-1399 | protein | denotes | PDZ-RhoGEF |
| T22 | 1451-1467 | protein | denotes | G protein family |
| T23 | 1483-1493 | protein | denotes | PDZ-RhoGEF |
| T24 | 1562-1577 | protein | denotes | Galpha12 family |
| T25 | 1579-1587 | protein | denotes | Galpha12 |
| T26 | 1592-1600 | protein | denotes | Galpha13 |
| T27 | 1709-1719 | protein | denotes | PDZ-RhoGEF |
| T28 | 1747-1750 | protein | denotes | Rho |
| T29 | 1754-1762 | protein | denotes | Galpha12 |
| T30 | 1767-1775 | protein | denotes | Galpha13 |
| T31 | 1926-1951 | protein | denotes | heterotrimeric G proteins |
| T32 | 1961-1964 | protein | denotes | Rho |
| T33 | 2031-2046 | protein | denotes | Galpha12 family |
| T34 | 2075-2090 | protein | denotes | exchange factor |
| T35 | 2101-2104 | protein | denotes | Rho |
bionlp-st-gro-2013-training
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 8-18 | ProteinDomain | denotes | PDZ domain |
| T4 | 71-96 | Protein | denotes | heterotrimeric G proteins |
| T5 | 100-103 | Protein | denotes | Rho |
| T9 | 139-142 | Protein | denotes | Rho |
| T12 | 273-285 | CellComponent | denotes | cell surface |
| T13 | 286-295 | Protein | denotes | receptors |
| T14 | 336-347 | ProteinDomain | denotes | Dbl domains |
| T15 | 351-390 | Protein | denotes | Rho guanine nucleotide exchange factors |
| T16 | 392-396 | Protein | denotes | GEFs |
| T17 | 408-411 | DNA | denotes | DNA |
| T18 | 447-452 | Eukaryote | denotes | human |
| T19 | 453-456 | Protein | denotes | GEF |
| T20 | 461-464 | Protein | denotes | Rho |
| T21 | 473-480 | Enzyme | denotes | GTPases |
| T22 | 568-575 | Protein | denotes | protein |
| T23 | 595-607 | ProteinDomain | denotes | DH/PH domain |
| T24 | 636-639 | Protein | denotes | Rho |
| T25 | 649-653 | Protein | denotes | GEFs |
| T26 | 657-667 | ProteinDomain | denotes | PDZ domain |
| T27 | 671-690 | ProteinDomain | denotes | proline-rich domain |
| T28 | 719-722 | Protein | denotes | Lsc |
| T29 | 724-735 | Protein | denotes | p115-RhoGEF |
| T30 | 743-753 | Eukaryote | denotes | Drosophila |
| T31 | 754-760 | Protein | denotes | RhoGEF |
| T32 | 791-793 | ProteinDomain | denotes | LH |
| T33 | 835-845 | Protein | denotes | PDZ-RhoGEF |
| T35 | 906-909 | Protein | denotes | Rho |
| T37 | 963-965 | ProteinDomain | denotes | DH |
| T38 | 970-979 | ProteinDomain | denotes | PH domain |
| T39 | 994-1004 | ProteinDomain | denotes | PDZ domain |
| T40 | 1046-1053 | MutantProtein | denotes | mutants |
| T41 | 1066-1075 | ProteinDomain | denotes | LH domain |
| T44 | 1140-1149 | ProteinDomain | denotes | LH domain |
| T45 | 1199-1208 | ProteinDomain | denotes | LH domain |
| T49 | 1315-1340 | Protein | denotes | heterotrimeric G proteins |
| T50 | 1342-1347 | Protein | denotes | RGS14 |
| T51 | 1389-1399 | Protein | denotes | PDZ-RhoGEF |
| T53 | 1451-1460 | Protein | denotes | G protein |
| T54 | 1483-1493 | Protein | denotes | PDZ-RhoGEF |
| T55 | 1562-1570 | Protein | denotes | Galpha12 |
| T56 | 1579-1587 | Protein | denotes | Galpha12 |
| T57 | 1592-1600 | Protein | denotes | Galpha13 |
| T60 | 1648-1657 | ProteinDomain | denotes | LH domain |
| T61 | 1709-1719 | Protein | denotes | PDZ-RhoGEF |
| T64 | 1747-1750 | Protein | denotes | Rho |
| T65 | 1754-1762 | Protein | denotes | Galpha12 |
| T66 | 1767-1775 | Protein | denotes | Galpha13 |
| T67 | 1873-1885 | CellComponent | denotes | cell surface |
| T68 | 1886-1895 | Protein | denotes | receptors |
| T69 | 1926-1951 | Protein | denotes | heterotrimeric G proteins |
| T73 | 2031-2039 | Protein | denotes | Galpha12 |
| T76 | 2101-2104 | Protein | denotes | Rho |
| T2 | 30-64 | Protein | denotes | guanine nucleotide exchange factor |
| T3 | 105-131 | Protein | denotes | Small GTP-binding proteins |
| T6 | 699-703 | ProteinDomain | denotes | area |
| T43 | 1285-1310 | Protein | denotes | GTPase-activating protein |
| T46 | 1528-1537 | ProteinComplex | denotes | complexes |
| T71 | 2075-2090 | Protein | denotes | exchange factor |
| E2 | 174-193 | SignalingPathway | denotes | signal transduction |
| E3 | 260-269 | PositiveRegulation | denotes | activated |
| E4 | 847-856 | PositiveRegulation | denotes | activated |
| E5 | 915-925 | PositiveRegulation | denotes | activation |
| E8 | 1406-1414 | BindingToProtein | denotes | interact |
| E9 | 1616-1627 | BindingToProtein | denotes | interaction |
| E10 | 1632-1640 | RegulatoryProcess | denotes | mediated |
| E11 | 1720-1728 | RegulatoryProcess | denotes | mediates |
| E12 | 1733-1743 | PositiveRegulation | denotes | activation |
| E13 | 1952-1960 | PositiveRegulation | denotes | activate |
| E14 | 1988-1999 | Increase | denotes | stimulating |
| E15 | 2063-2071 | PositiveRegulation | denotes | activate |
| E1 | 857-892 | Pathway | denotes | biological and biochemical pathways |
| E6 | 1107-1126 | NegativeRegulation | denotes | negative regulatory |
| E16 | 935-943 | Pathway | denotes | pathways |
| E7 | 1901-1917 | SignalingPathway | denotes | transmit signals |
| E17 | 1961-1983 | SignalingPathway | denotes | Rho-dependent pathways |
| E18 | 1733-1743 | PositiveRegulation | denotes | activation |
| E19 | 1720-1728 | RegulatoryProcess | denotes | mediates |
| R1 | T13 | T12 | locatedIn | receptors,cell surface |
| R3 | T19 | T18 | fromSpecies | GEF,human |
| R6 | T22 | T23 | hasPart | protein,DH/PH domain |
| R7 | T22 | T26 | hasPart | protein,PDZ domain |
| R8 | T22 | T27 | hasPart | protein,proline-rich domain |
| R9 | T31 | T30 | fromSpecies | RhoGEF,Drosophila |
| R10 | T68 | T67 | locatedIn | receptors,cell surface |
| R13 | T2 | T1 | hasPart | guanine nucleotide exchange factor,PDZ domain |
| R2 | T15 | T14 | hasPart | Rho guanine nucleotide exchange factors,Dbl domains |
| R4 | T22 | T6 | hasPart | protein,area |
| R5 | T46 | T54 | hasPart | complexes,PDZ-RhoGEF |
| R14 | T46 | T56 | hasPart | complexes,Galpha12 |
| R15 | T46 | T57 | hasPart | complexes,Galpha13 |
| R17 | T13 | E3 | hasAgent | receptors,activated |
| R18 | T33 | E4 | hasAgent | PDZ-RhoGEF,activated |
| R19 | E1 | E4 | hasPatient | biological and biochemical pathways,activated |
| R20 | E16 | E5 | hasPatient | pathways,activation |
| R21 | T53 | E8 | hasPatient | G protein,interact |
| R22 | T51 | E8 | hasPatient | PDZ-RhoGEF,interact |
| R23 | T60 | E10 | hasAgent | LH domain,mediated |
| R24 | E9 | E10 | hasPatient | interaction,mediated |
| R25 | T61 | E11 | hasAgent | PDZ-RhoGEF,mediates |
| R26 | E12 | E11 | hasPatient | activation,mediates |
| R27 | T65 | E12 | hasAgent | Galpha12,activation |
| R28 | T64 | E12 | hasPatient | Rho,activation |
| R29 | T68 | E13 | hasAgent | receptors,activate |
| R30 | E17 | E13 | hasPatient | Rho-dependent pathways,activate |
| R31 | T73 | E14 | hasPatient | Galpha12,stimulating |
| R32 | T71 | E15 | hasPatient | exchange factor,activate |
| R33 | T35 | E1 | hasPatient | Rho,biological and biochemical pathways |
| R34 | T44 | E6 | hasAgent | LH domain,negative regulatory |
| R35 | T69 | E7 | hasAgent | heterotrimeric G proteins,transmit signals |
| R36 | T66 | E18 | hasAgent | Galpha13,activation |
| R37 | T64 | E18 | hasPatient | Rho,activation |
| R38 | T61 | E19 | hasAgent | PDZ-RhoGEF,mediates |
| R39 | E18 | E19 | hasPatient | activation,mediates |
mondo_disease
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T1 | 392-396 | Disease | denotes | GEFs | http://purl.obolibrary.org/obo/MONDO_0018214 |
| T2 | 649-653 | Disease | denotes | GEFs | http://purl.obolibrary.org/obo/MONDO_0018214 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 447-452 | OrganismTaxon | denotes | human | 9606 |
| T2 | 743-753 | OrganismTaxon | denotes | Drosophila | 2081351|32281|7215 |