PubMed:10021369 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10021369","sourcedb":"PubMed","sourceid":"10021369","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10021369","text":"Identification of APC2, a homologue of the adenomatous polyposis coli tumour suppressor.\nThe adenomatous polyposis coli (APC) tumour-suppressor protein controls the Wnt signalling pathway by forming a complex with glycogen synthase kinase 3beta (GSK-3beta), axin/conductin and betacatenin. Complex formation induces the rapid degradation of betacatenin. In colon carcinoma cells, loss of APC leads to the accumulation of betacatenin in the nucleus, where it binds to and activates the Tcf-4 transcription factor (reviewed in [1] [2]). Here, we report the identification and genomic structure of APC homologues. Mammalian APC2, which closely resembles APC in overall domain structure, was functionally analyzed and shown to contain two SAMP domains, both of which are required for binding to conductin. Like APC, APC2 regulates the formation of active betacatenin-Tcf complexes, as demonstrated using transient transcriptional activation assays in APC -/- colon carcinoma cells. Human APC2 maps to chromosome 19p13.3. APC and APC2 may therefore have comparable functions in development and cancer.","tracks":[{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":812,"end":816},"obj":"gene:344"},{"id":"T1","span":{"begin":947,"end":950},"obj":"disease:C0032580"},{"id":"T2","span":{"begin":812,"end":816},"obj":"gene:29882"},{"id":"T3","span":{"begin":947,"end":950},"obj":"disease:C0032580"},{"id":"T4","span":{"begin":812,"end":816},"obj":"gene:79085"},{"id":"T5","span":{"begin":947,"end":950},"obj":"disease:C0032580"},{"id":"T6","span":{"begin":812,"end":816},"obj":"gene:10297"},{"id":"T7","span":{"begin":947,"end":950},"obj":"disease:C0032580"},{"id":"T8","span":{"begin":43,"end":54},"obj":"disease:C0032580"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"},{"id":"R3","pred":"associated_with","subj":"T4","obj":"T5"},{"id":"R4","pred":"associated_with","subj":"T6","obj":"T7"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"attributes":[{"subj":"T0","pred":"source","obj":"DisGeNET"},{"subj":"T1","pred":"source","obj":"DisGeNET"},{"subj":"T2","pred":"source","obj":"DisGeNET"},{"subj":"T3","pred":"source","obj":"DisGeNET"},{"subj":"T4","pred":"source","obj":"DisGeNET"},{"subj":"T5","pred":"source","obj":"DisGeNET"},{"subj":"T6","pred":"source","obj":"DisGeNET"},{"subj":"T7","pred":"source","obj":"DisGeNET"},{"subj":"T8","pred":"source","obj":"DisGeNET"}]},{"project":"DisGeNET5_gene_disease","denotations":[{"id":"10021369-0#18#22#gene10297","span":{"begin":18,"end":22},"obj":"gene10297"},{"id":"10021369-0#70#87#gene7248","span":{"begin":70,"end":87},"obj":"gene7248"},{"id":"10021369-0#43#69#diseaseC0032580","span":{"begin":43,"end":69},"obj":"diseaseC0032580"},{"id":"10021369-1#37#62#gene84260","span":{"begin":126,"end":151},"obj":"gene84260"},{"id":"10021369-1#4#30#diseaseC0032580","span":{"begin":93,"end":119},"obj":"diseaseC0032580"},{"id":"10021369-1#32#35#diseaseC0032580","span":{"begin":121,"end":124},"obj":"diseaseC0032580"},{"id":"10021369-3#131#136#gene6925","span":{"begin":485,"end":490},"obj":"gene6925"},{"id":"10021369-3#131#136#gene6934","span":{"begin":485,"end":490},"obj":"gene6934"},{"id":"10021369-3#34#37#diseaseC0032580","span":{"begin":388,"end":391},"obj":"diseaseC0032580"},{"id":"10021369-5#124#128#gene7511","span":{"begin":735,"end":739},"obj":"gene7511"},{"id":"10021369-5#180#189#gene8313","span":{"begin":791,"end":800},"obj":"gene8313"},{"id":"10021369-5#40#43#diseaseC0032580","span":{"begin":651,"end":654},"obj":"diseaseC0032580"},{"id":"10021369-6#181#185#gene10297","span":{"begin":984,"end":988},"obj":"gene10297"},{"id":"10021369-6#153#168#diseaseC0699790","span":{"begin":955,"end":970},"obj":"diseaseC0699790"},{"id":"10021369-7#8#12#gene10297","span":{"begin":1025,"end":1029},"obj":"gene10297"},{"id":"10021369-7#0#3#diseaseC0033036","span":{"begin":1017,"end":1020},"obj":"diseaseC0033036"}],"relations":[{"id":"18#22#gene1029743#69#diseaseC0032580","pred":"associated_with","subj":"10021369-0#18#22#gene10297","obj":"10021369-0#43#69#diseaseC0032580"},{"id":"70#87#gene724843#69#diseaseC0032580","pred":"associated_with","subj":"10021369-0#70#87#gene7248","obj":"10021369-0#43#69#diseaseC0032580"},{"id":"37#62#gene842604#30#diseaseC0032580","pred":"associated_with","subj":"10021369-1#37#62#gene84260","obj":"10021369-1#4#30#diseaseC0032580"},{"id":"37#62#gene8426032#35#diseaseC0032580","pred":"associated_with","subj":"10021369-1#37#62#gene84260","obj":"10021369-1#32#35#diseaseC0032580"},{"id":"131#136#gene692534#37#diseaseC0032580","pred":"associated_with","subj":"10021369-3#131#136#gene6925","obj":"10021369-3#34#37#diseaseC0032580"},{"id":"131#136#gene693434#37#diseaseC0032580","pred":"associated_with","subj":"10021369-3#131#136#gene6934","obj":"10021369-3#34#37#diseaseC0032580"},{"id":"124#128#gene751140#43#diseaseC0032580","pred":"associated_with","subj":"10021369-5#124#128#gene7511","obj":"10021369-5#40#43#diseaseC0032580"},{"id":"180#189#gene831340#43#diseaseC0032580","pred":"associated_with","subj":"10021369-5#180#189#gene8313","obj":"10021369-5#40#43#diseaseC0032580"},{"id":"181#185#gene10297153#168#diseaseC0699790","pred":"associated_with","subj":"10021369-6#181#185#gene10297","obj":"10021369-6#153#168#diseaseC0699790"},{"id":"8#12#gene102970#3#diseaseC0033036","pred":"associated_with","subj":"10021369-7#8#12#gene10297","obj":"10021369-7#0#3#diseaseC0033036"}],"attributes":[{"subj":"10021369-0#18#22#gene10297","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"10021369-0#70#87#gene7248","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"10021369-0#43#69#diseaseC0032580","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"10021369-1#37#62#gene84260","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"10021369-1#4#30#diseaseC0032580","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"10021369-1#32#35#diseaseC0032580","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"10021369-3#131#136#gene6925","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"10021369-3#131#136#gene6934","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"10021369-3#34#37#diseaseC0032580","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"10021369-5#124#128#gene7511","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"10021369-5#180#189#gene8313","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"10021369-5#40#43#diseaseC0032580","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"10021369-6#181#185#gene10297","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"10021369-6#153#168#diseaseC0699790","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"10021369-7#8#12#gene10297","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"10021369-7#0#3#diseaseC0033036","pred":"source","obj":"DisGeNET5_gene_disease"}]},{"project":"bionlp-st-gro-2013-development","denotations":[{"id":"T1","span":{"begin":18,"end":22},"obj":"Protein"},{"id":"T8","span":{"begin":165,"end":168},"obj":"Protein"},{"id":"T11","span":{"begin":201,"end":208},"obj":"ProteinComplex"},{"id":"T13","span":{"begin":246,"end":255},"obj":"Enzyme"},{"id":"T14","span":{"begin":258,"end":272},"obj":"Protein"},{"id":"T15","span":{"begin":277,"end":288},"obj":"Protein"},{"id":"T16","span":{"begin":290,"end":297},"obj":"ProteinComplex"},{"id":"T20","span":{"begin":341,"end":352},"obj":"Protein"},{"id":"T23","span":{"begin":388,"end":391},"obj":"Protein"},{"id":"T25","span":{"begin":421,"end":432},"obj":"Protein"},{"id":"T26","span":{"begin":440,"end":447},"obj":"Nucleus"},{"id":"T31","span":{"begin":595,"end":598},"obj":"Protein"},{"id":"T32","span":{"begin":611,"end":620},"obj":"Eukaryote"},{"id":"T33","span":{"begin":621,"end":625},"obj":"Protein"},{"id":"T34","span":{"begin":651,"end":654},"obj":"Protein"},{"id":"T35","span":{"begin":666,"end":682},"obj":"ProteinDomain"},{"id":"T36","span":{"begin":735,"end":747},"obj":"ProteinDomain"},{"id":"T38","span":{"begin":791,"end":800},"obj":"Protein"},{"id":"T39","span":{"begin":807,"end":810},"obj":"Protein"},{"id":"T40","span":{"begin":812,"end":816},"obj":"Protein"},{"id":"T43","span":{"begin":851,"end":862},"obj":"Protein"},{"id":"T44","span":{"begin":863,"end":866},"obj":"Protein"},{"id":"T45","span":{"begin":867,"end":876},"obj":"ProteinComplex"},{"id":"T48","span":{"begin":947,"end":950},"obj":"Protein"},{"id":"T50","span":{"begin":978,"end":983},"obj":"Eukaryote"},{"id":"T51","span":{"begin":984,"end":988},"obj":"Protein"},{"id":"T52","span":{"begin":997,"end":1015},"obj":"Chromosome"},{"id":"T53","span":{"begin":1017,"end":1020},"obj":"Protein"},{"id":"T54","span":{"begin":1025,"end":1029},"obj":"Protein"},{"id":"T2","span":{"begin":43,"end":87},"obj":"Protein"},{"id":"T5","span":{"begin":373,"end":378},"obj":"Cell"},{"id":"T4","span":{"begin":357,"end":372},"obj":"Tissue"},{"id":"T6","span":{"begin":955,"end":970},"obj":"Tissue"},{"id":"T21","span":{"begin":971,"end":976},"obj":"Cell"},{"id":"T49","span":{"begin":1060,"end":1069},"obj":"Function"},{"id":"T55","span":{"begin":937,"end":943},"obj":"ExperimentalMethod"},{"id":"T3","span":{"begin":93,"end":151},"obj":"Protein"},{"id":"T17","span":{"begin":485,"end":511},"obj":"TranscriptionFactor"},{"id":"E3","span":{"begin":152,"end":160},"obj":"RegulatoryProcess"},{"id":"E4","span":{"begin":169,"end":187},"obj":"SignalingPathway"},{"id":"E7","span":{"begin":308,"end":315},"obj":"PositiveRegulation"},{"id":"E8","span":{"begin":326,"end":337},"obj":"ProteinCatabolism"},{"id":"E9","span":{"begin":380,"end":384},"obj":"Decrease"},{"id":"E10","span":{"begin":405,"end":417},"obj":"Increase"},{"id":"E11","span":{"begin":458,"end":463},"obj":"BindingToProtein"},{"id":"E12","span":{"begin":471,"end":480},"obj":"PositiveRegulation"},{"id":"E13","span":{"begin":780,"end":787},"obj":"BindingToProtein"},{"id":"E14","span":{"begin":817,"end":826},"obj":"RegulatoryProcess"},{"id":"E15","span":{"begin":831,"end":840},"obj":"FormationOfTranscriptionFactorComplex"},{"id":"E16","span":{"begin":910,"end":925},"obj":"Transcription"},{"id":"E17","span":{"begin":926,"end":936},"obj":"PositiveRegulation"},{"id":"E18","span":{"begin":1073,"end":1084},"obj":"DevelopmentalProcess"},{"id":"E19","span":{"begin":1089,"end":1095},"obj":"Disease"},{"id":"E1","span":{"begin":392,"end":397},"obj":"PositiveRegulation"}],"relations":[{"id":"R3","pred":"hasPart","subj":"T11","obj":"T14"},{"id":"R4","pred":"hasPart","subj":"T11","obj":"T15"},{"id":"R7","pred":"locatedIn","subj":"E10","obj":"T26"},{"id":"R8","pred":"fromSpecies","subj":"T33","obj":"T32"},{"id":"R9","pred":"hasPart","subj":"T45","obj":"T43"},{"id":"R10","pred":"hasPart","subj":"T45","obj":"T44"},{"id":"R12","pred":"locatedIn","subj":"T51","obj":"T52"},{"id":"R13","pred":"fromSpecies","subj":"T51","obj":"T50"},{"id":"R5","pred":"locatedIn","subj":"T5","obj":"T4"},{"id":"R17","pred":"locatedIn","subj":"E9","obj":"T5"},{"id":"R11","pred":"locatedIn","subj":"T21","obj":"T6"},{"id":"R23","pred":"locatedIn","subj":"T48","obj":"T21"},{"id":"R1","pred":"hasPart","subj":"T33","obj":"T36"},{"id":"R6","pred":"hasPart","subj":"T11","obj":"T3"},{"id":"R14","pred":"hasFunction","subj":"T54","obj":"T49"},{"id":"R15","pred":"hasFunction","subj":"T53","obj":"T49"},{"id":"R16","pred":"hasPart","subj":"T11","obj":"T13"},{"id":"R18","pred":"hasAgent","subj":"T3","obj":"E3"},{"id":"R19","pred":"hasPatient","subj":"E4","obj":"E3"},{"id":"R20","pred":"hasAgent","subj":"T16","obj":"E7"},{"id":"R21","pred":"hasPatient","subj":"E8","obj":"E7"},{"id":"R22","pred":"hasPatient","subj":"T20","obj":"E8"},{"id":"R23","pred":"hasPatient","subj":"T23","obj":"E9"},{"id":"R24","pred":"hasPatient","subj":"T25","obj":"E10"},{"id":"R25","pred":"hasPatient","subj":"T17","obj":"E11"},{"id":"R26","pred":"hasPatient","subj":"T17","obj":"E12"},{"id":"R28","pred":"hasPatient","subj":"T38","obj":"E13"},{"id":"R29","pred":"hasAgent","subj":"T40","obj":"E14"},{"id":"R30","pred":"hasPatient","subj":"E15","obj":"E14"},{"id":"R31","pred":"hasPatient","subj":"T45","obj":"E15"},{"id":"R32","pred":"hasPatient","subj":"E16","obj":"E17"},{"id":"R33","pred":"hasAgent","subj":"E9","obj":"E1"},{"id":"R34","pred":"hasPatient","subj":"E10","obj":"E1"}],"attributes":[{"subj":"T1","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T8","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T11","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T13","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T14","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T15","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T16","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T20","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T23","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T25","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T26","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T31","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T32","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T33","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T34","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T35","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T36","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T38","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T39","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T40","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T43","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T44","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T45","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T48","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T50","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T51","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T52","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T53","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T54","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T2","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T5","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T4","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T6","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T21","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T49","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T55","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T3","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"T17","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"E3","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"E4","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"E7","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"E8","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"E9","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"E10","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"E11","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"E12","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"E13","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"E14","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"E15","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"E16","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"E17","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"E18","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"E19","pred":"source","obj":"bionlp-st-gro-2013-development"},{"subj":"E1","pred":"source","obj":"bionlp-st-gro-2013-development"}]},{"project":"NCBIDiseaseCorpus","denotations":[{"id":"T1","span":{"begin":43,"end":76},"obj":"Modifier:D011125"},{"id":"T2","span":{"begin":93,"end":132},"obj":"Modifier:D011125"},{"id":"T3","span":{"begin":357,"end":372},"obj":"Modifier:D003110"},{"id":"T4","span":{"begin":955,"end":970},"obj":"Modifier:D003110"},{"id":"T5","span":{"begin":1089,"end":1095},"obj":"SpecificDisease:D009369"}],"attributes":[{"subj":"T1","pred":"source","obj":"NCBIDiseaseCorpus"},{"subj":"T2","pred":"source","obj":"NCBIDiseaseCorpus"},{"subj":"T3","pred":"source","obj":"NCBIDiseaseCorpus"},{"subj":"T4","pred":"source","obj":"NCBIDiseaseCorpus"},{"subj":"T5","pred":"source","obj":"NCBIDiseaseCorpus"}]},{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":126,"end":132},"obj":"HP_0002664"},{"id":"T2","span":{"begin":1089,"end":1095},"obj":"HP_0002664"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"},{"subj":"T2","pred":"source","obj":"PubmedHPO"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"DisGeNET","color":"#93cdec","default":true},{"id":"DisGeNET5_gene_disease","color":"#e7ec93"},{"id":"bionlp-st-gro-2013-development","color":"#d693ec"},{"id":"NCBIDiseaseCorpus","color":"#93ecbc"},{"id":"PubmedHPO","color":"#eca293"}]}]}}