PMC:88885 / 8964-9586 JSONTXT

{"project":"craft-sa-dev","denotations":[{"id":"T2267","span":{"begin":0,"end":2},"obj":"IN"},{"id":"T2268","span":{"begin":139,"end":148},"obj":"VBN"},{"id":"T2269","span":{"begin":3,"end":8},"obj":"NN"},{"id":"T2270","span":{"begin":9,"end":11},"obj":"TO"},{"id":"T2271","span":{"begin":12,"end":21},"obj":"VB"},{"id":"T2272","span":{"begin":22,"end":25},"obj":"DT"},{"id":"T2273","span":{"begin":26,"end":34},"obj":"NN"},{"id":"T2274","span":{"begin":35,"end":37},"obj":"IN"},{"id":"T2275","span":{"begin":38,"end":41},"obj":"DT"},{"id":"T2276","span":{"begin":49,"end":59},"obj":"NN"},{"id":"T2277","span":{"begin":42,"end":48},"obj":"JJ"},{"id":"T2278","span":{"begin":59,"end":61},"obj":","},{"id":"T2279","span":{"begin":61,"end":63},"obj":"NN"},{"id":"T2280","span":{"begin":64,"end":67},"obj":"NN"},{"id":"T2281","span":{"begin":63,"end":64},"obj":"HYPH"},{"id":"T2282","span":{"begin":68,"end":73},"obj":"VBG"},{"id":"T2283","span":{"begin":74,"end":81},"obj":"NNS"},{"id":"T2284","span":{"begin":82,"end":84},"obj":"IN"},{"id":"T2285","span":{"begin":85,"end":90},"obj":"NNS"},{"id":"T2286","span":{"begin":91,"end":93},"obj":"CD"},{"id":"T2287","span":{"begin":94,"end":97},"obj":"CC"},{"id":"T2288","span":{"begin":98,"end":100},"obj":"CD"},{"id":"T2289","span":{"begin":101,"end":107},"obj":"VBN"},{"id":"T2290","span":{"begin":108,"end":112},"obj":"IN"},{"id":"T2291","span":{"begin":113,"end":114},"obj":"DT"},{"id":"T2292","span":{"begin":115,"end":121},"obj":"NN"},{"id":"T2293","span":{"begin":122,"end":124},"obj":"IN"},{"id":"T2294","span":{"begin":125,"end":131},"obj":"NN"},{"id":"T2295","span":{"begin":132,"end":134},"obj":"CD"},{"id":"T2296","span":{"begin":135,"end":138},"obj":"VBD"},{"id":"T2297","span":{"begin":149,"end":154},"obj":"VBG"},{"id":"T2298","span":{"begin":155,"end":159},"obj":"NN"},{"id":"T2299","span":{"begin":160,"end":161},"obj":"NN"},{"id":"T2300","span":{"begin":159,"end":160},"obj":"HYPH"},{"id":"T2301","span":{"begin":162,"end":167},"obj":"NN"},{"id":"T2302","span":{"begin":168,"end":173},"obj":"NN"},{"id":"T2303","span":{"begin":180,"end":183},"obj":"NN"},{"id":"T2304","span":{"begin":174,"end":179},"obj":"JJ"},{"id":"T2305","span":{"begin":184,"end":187},"obj":"CC"},{"id":"T2306","span":{"begin":188,"end":191},"obj":"DT"},{"id":"T2307","span":{"begin":202,"end":210},"obj":"NNS"},{"id":"T2308","span":{"begin":192,"end":201},"obj":"VBG"},{"id":"T2309","span":{"begin":211,"end":220},"obj":"VBN"},{"id":"T2310","span":{"begin":220,"end":221},"obj":"."},{"id":"T2311","span":{"begin":221,"end":343},"obj":"sentence"},{"id":"T2312","span":{"begin":222,"end":227},"obj":"DT"},{"id":"T2313","span":{"begin":228,"end":236},"obj":"NNS"},{"id":"T2314","span":{"begin":237,"end":241},"obj":"VBP"},{"id":"T2315","span":{"begin":242,"end":246},"obj":"IN"},{"id":"T2316","span":{"begin":269,"end":271},"obj":"VBZ"},{"id":"T2317","span":{"begin":247,"end":250},"obj":"DT"},{"id":"T2318","span":{"begin":258,"end":268},"obj":"NN"},{"id":"T2319","span":{"begin":251,"end":257},"obj":"JJR"},{"id":"T2320","span":{"begin":272,"end":275},"obj":"IN"},{"id":"T2321","span":{"begin":276,"end":278},"obj":"IN"},{"id":"T2322","span":{"begin":279,"end":281},"obj":"DT"},{"id":"T2323","span":{"begin":301,"end":306},"obj":"NN"},{"id":"T2324","span":{"begin":282,"end":293},"obj":"JJ"},{"id":"T2325","span":{"begin":294,"end":300},"obj":"NN"},{"id":"T2326","span":{"begin":307,"end":311},"obj":"WDT"},{"id":"T2327","span":{"begin":312,"end":319},"obj":"VBZ"},{"id":"T2328","span":{"begin":320,"end":322},"obj":"IN"},{"id":"T2329","span":{"begin":323,"end":325},"obj":"DT"},{"id":"T2330","span":{"begin":335,"end":339},"obj":"NN"},{"id":"T2331","span":{"begin":326,"end":334},"obj":"VBN"},{"id":"T2332","span":{"begin":340,"end":342},"obj":"CD"},{"id":"T2333","span":{"begin":342,"end":343},"obj":"."},{"id":"T2334","span":{"begin":343,"end":465},"obj":"sentence"},{"id":"T2335","span":{"begin":344,"end":356},"obj":"RB"},{"id":"T2336","span":{"begin":366,"end":373},"obj":"VBZ"},{"id":"T2337","span":{"begin":356,"end":358},"obj":","},{"id":"T2338","span":{"begin":358,"end":362},"obj":"NN"},{"id":"T2339","span":{"begin":363,"end":365},"obj":"CD"},{"id":"T2340","span":{"begin":374,"end":376},"obj":"IN"},{"id":"T2341","span":{"begin":377,"end":379},"obj":"NN"},{"id":"T2342","span":{"begin":380,"end":383},"obj":"CD"},{"id":"T2343","span":{"begin":384,"end":386},"obj":"IN"},{"id":"T2344","span":{"begin":387,"end":393},"obj":"NN"},{"id":"T2345","span":{"begin":394,"end":396},"obj":"CD"},{"id":"T2346","span":{"begin":396,"end":398},"obj":","},{"id":"T2347","span":{"begin":398,"end":406},"obj":"VBG"},{"id":"T2348","span":{"begin":407,"end":408},"obj":"DT"},{"id":"T2349","span":{"begin":423,"end":427},"obj":"NN"},{"id":"T2350","span":{"begin":409,"end":414},"obj":"JJ"},{"id":"T2351","span":{"begin":415,"end":419},"obj":"CD"},{"id":"T2352","span":{"begin":420,"end":422},"obj":"NN"},{"id":"T2353","span":{"begin":428,"end":430},"obj":"CD"},{"id":"T2354","span":{"begin":431,"end":435},"obj":"WDT"},{"id":"T2355","span":{"begin":436,"end":443},"obj":"VBZ"},{"id":"T2356","span":{"begin":444,"end":453},"obj":"RB"},{"id":"T2357","span":{"begin":454,"end":456},"obj":"IN"},{"id":"T2358","span":{"begin":457,"end":461},"obj":"NN"},{"id":"T2359","span":{"begin":462,"end":464},"obj":"CD"},{"id":"T2360","span":{"begin":464,"end":465},"obj":"."},{"id":"T2361","span":{"begin":465,"end":622},"obj":"sentence"},{"id":"T2362","span":{"begin":466,"end":469},"obj":"DT"},{"id":"T2363","span":{"begin":483,"end":488},"obj":"NN"},{"id":"T2364","span":{"begin":470,"end":474},"obj":"JJ"},{"id":"T2365","span":{"begin":475,"end":482},"obj":"NN"},{"id":"T2366","span":{"begin":530,"end":532},"obj":"VBZ"},{"id":"T2367","span":{"begin":489,"end":491},"obj":"IN"},{"id":"T2368","span":{"begin":492,"end":496},"obj":"DT"},{"id":"T2369","span":{"begin":519,"end":529},"obj":"NN"},{"id":"T2370","span":{"begin":497,"end":510},"obj":"RB"},{"id":"T2371","span":{"begin":511,"end":518},"obj":"VBN"},{"id":"T2372","span":{"begin":533,"end":536},"obj":"CD"},{"id":"T2373","span":{"begin":543,"end":548},"obj":"NNS"},{"id":"T2374","span":{"begin":537,"end":542},"obj":"NN"},{"id":"T2375","span":{"begin":548,"end":550},"obj":","},{"id":"T2376","span":{"begin":550,"end":552},"obj":"CD"},{"id":"T2377","span":{"begin":559,"end":564},"obj":"NNS"},{"id":"T2378","span":{"begin":553,"end":558},"obj":"JJ"},{"id":"T2379","span":{"begin":565,"end":571},"obj":"JJR"},{"id":"T2380","span":{"begin":572,"end":576},"obj":"IN"},{"id":"T2381","span":{"begin":577,"end":580},"obj":"DT"},{"id":"T2382","span":{"begin":581,"end":588},"obj":"NN"},{"id":"T2383","span":{"begin":589,"end":596},"obj":"VBN"},{"id":"T2384","span":{"begin":597,"end":599},"obj":"IN"},{"id":"T2385","span":{"begin":600,"end":603},"obj":"DT"},{"id":"T2386","span":{"begin":611,"end":621},"obj":"NN"},{"id":"T2387","span":{"begin":604,"end":607},"obj":"CD"},{"id":"T2388","span":{"begin":608,"end":610},"obj":"NN"},{"id":"T2389","span":{"begin":621,"end":622},"obj":"."}],"relations":[{"id":"R1350","pred":"prep","subj":"T2267","obj":"T2268"},{"id":"R1351","pred":"pobj","subj":"T2269","obj":"T2267"},{"id":"R1352","pred":"aux","subj":"T2270","obj":"T2271"},{"id":"R1353","pred":"acl","subj":"T2271","obj":"T2269"},{"id":"R1354","pred":"det","subj":"T2272","obj":"T2273"},{"id":"R1355","pred":"dobj","subj":"T2273","obj":"T2271"},{"id":"R1356","pred":"prep","subj":"T2274","obj":"T2273"},{"id":"R1357","pred":"det","subj":"T2275","obj":"T2276"},{"id":"R1358","pred":"pobj","subj":"T2276","obj":"T2274"},{"id":"R1359","pred":"amod","subj":"T2277","obj":"T2276"},{"id":"R1360","pred":"punct","subj":"T2278","obj":"T2268"},{"id":"R1361","pred":"compound","subj":"T2279","obj":"T2280"},{"id":"R1362","pred":"nsubjpass","subj":"T2280","obj":"T2268"},{"id":"R1363","pred":"punct","subj":"T2281","obj":"T2280"},{"id":"R1364","pred":"acl","subj":"T2282","obj":"T2280"},{"id":"R1365","pred":"dobj","subj":"T2283","obj":"T2282"},{"id":"R1366","pred":"prep","subj":"T2284","obj":"T2283"},{"id":"R1367","pred":"nmod","subj":"T2285","obj":"T2286"},{"id":"R1368","pred":"pobj","subj":"T2286","obj":"T2284"},{"id":"R1369","pred":"cc","subj":"T2287","obj":"T2286"},{"id":"R1370","pred":"conj","subj":"T2288","obj":"T2286"},{"id":"R1371","pred":"acl","subj":"T2289","obj":"T2283"},{"id":"R1372","pred":"prep","subj":"T2290","obj":"T2289"},{"id":"R1373","pred":"det","subj":"T2291","obj":"T2292"},{"id":"R1374","pred":"pobj","subj":"T2292","obj":"T2290"},{"id":"R1375","pred":"prep","subj":"T2293","obj":"T2292"},{"id":"R1376","pred":"pobj","subj":"T2294","obj":"T2293"},{"id":"R1377","pred":"nummod","subj":"T2295","obj":"T2294"},{"id":"R1378","pred":"auxpass","subj":"T2296","obj":"T2268"},{"id":"R1379","pred":"advcl","subj":"T2297","obj":"T2268"},{"id":"R1380","pred":"nmod","subj":"T2298","obj":"T2299"},{"id":"R1381","pred":"nmod","subj":"T2299","obj":"T2301"},{"id":"R1382","pred":"punct","subj":"T2300","obj":"T2299"},{"id":"R1383","pred":"nmod","subj":"T2301","obj":"T2302"},{"id":"R1384","pred":"nmod","subj":"T2302","obj":"T2303"},{"id":"R1385","pred":"dobj","subj":"T2303","obj":"T2297"},{"id":"R1386","pred":"amod","subj":"T2304","obj":"T2303"},{"id":"R1387","pred":"cc","subj":"T2305","obj":"T2268"},{"id":"R1388","pred":"det","subj":"T2306","obj":"T2307"},{"id":"R1389","pred":"nsubj","subj":"T2307","obj":"T2309"},{"id":"R1390","pred":"amod","subj":"T2308","obj":"T2307"},{"id":"R1391","pred":"conj","subj":"T2309","obj":"T2268"},{"id":"R1392","pred":"punct","subj":"T2310","obj":"T2268"},{"id":"R1393","pred":"det","subj":"T2312","obj":"T2313"},{"id":"R1394","pred":"nsubj","subj":"T2313","obj":"T2314"},{"id":"R1395","pred":"mark","subj":"T2315","obj":"T2316"},{"id":"R1396","pred":"ccomp","subj":"T2316","obj":"T2314"},{"id":"R1397","pred":"det","subj":"T2317","obj":"T2318"},{"id":"R1398","pred":"nsubj","subj":"T2318","obj":"T2316"},{"id":"R1399","pred":"amod","subj":"T2319","obj":"T2318"},{"id":"R1400","pred":"prep","subj":"T2320","obj":"T2316"},{"id":"R1401","pred":"pcomp","subj":"T2321","obj":"T2320"},{"id":"R1402","pred":"det","subj":"T2322","obj":"T2323"},{"id":"R1403","pred":"pobj","subj":"T2323","obj":"T2320"},{"id":"R1404","pred":"amod","subj":"T2324","obj":"T2323"},{"id":"R1405","pred":"compound","subj":"T2325","obj":"T2323"},{"id":"R1406","pred":"dep","subj":"T2326","obj":"T2327"},{"id":"R1407","pred":"relcl","subj":"T2327","obj":"T2323"},{"id":"R1408","pred":"prep","subj":"T2328","obj":"T2327"},{"id":"R1409","pred":"det","subj":"T2329","obj":"T2330"},{"id":"R1410","pred":"pobj","subj":"T2330","obj":"T2328"},{"id":"R1411","pred":"amod","subj":"T2331","obj":"T2330"},{"id":"R1412","pred":"nummod","subj":"T2332","obj":"T2330"},{"id":"R1413","pred":"punct","subj":"T2333","obj":"T2314"},{"id":"R1414","pred":"advmod","subj":"T2335","obj":"T2336"},{"id":"R1415","pred":"punct","subj":"T2337","obj":"T2336"},{"id":"R1416","pred":"nsubj","subj":"T2338","obj":"T2336"},{"id":"R1417","pred":"nummod","subj":"T2339","obj":"T2338"},{"id":"R1418","pred":"prep","subj":"T2340","obj":"T2336"},{"id":"R1419","pred":"pobj","subj":"T2341","obj":"T2340"},{"id":"R1420","pred":"nummod","subj":"T2342","obj":"T2341"},{"id":"R1421","pred":"prep","subj":"T2343","obj":"T2341"},{"id":"R1422","pred":"pobj","subj":"T2344","obj":"T2343"},{"id":"R1423","pred":"nummod","subj":"T2345","obj":"T2344"},{"id":"R1424","pred":"punct","subj":"T2346","obj":"T2336"},{"id":"R1425","pred":"advcl","subj":"T2347","obj":"T2336"},{"id":"R1426","pred":"det","subj":"T2348","obj":"T2349"},{"id":"R1427","pred":"dobj","subj":"T2349","obj":"T2347"},{"id":"R1428","pred":"amod","subj":"T2350","obj":"T2349"},{"id":"R1429","pred":"nummod","subj":"T2351","obj":"T2352"},{"id":"R1430","pred":"compound","subj":"T2352","obj":"T2349"},{"id":"R1431","pred":"nummod","subj":"T2353","obj":"T2349"},{"id":"R1432","pred":"dep","subj":"T2354","obj":"T2355"},{"id":"R1433","pred":"relcl","subj":"T2355","obj":"T2349"},{"id":"R1434","pred":"advmod","subj":"T2356","obj":"T2355"},{"id":"R1435","pred":"prep","subj":"T2357","obj":"T2355"},{"id":"R1436","pred":"pobj","subj":"T2358","obj":"T2357"},{"id":"R1437","pred":"nummod","subj":"T2359","obj":"T2358"},{"id":"R1438","pred":"punct","subj":"T2360","obj":"T2336"},{"id":"R1439","pred":"det","subj":"T2362","obj":"T2363"},{"id":"R1440","pred":"nsubj","subj":"T2363","obj":"T2366"},{"id":"R1441","pred":"amod","subj":"T2364","obj":"T2363"},{"id":"R1442","pred":"compound","subj":"T2365","obj":"T2363"},{"id":"R1443","pred":"prep","subj":"T2367","obj":"T2363"},{"id":"R1444","pred":"det","subj":"T2368","obj":"T2369"},{"id":"R1445","pred":"pobj","subj":"T2369","obj":"T2367"},{"id":"R1446","pred":"advmod","subj":"T2370","obj":"T2371"},{"id":"R1447","pred":"amod","subj":"T2371","obj":"T2369"},{"id":"R1448","pred":"nummod","subj":"T2372","obj":"T2373"},{"id":"R1449","pred":"attr","subj":"T2373","obj":"T2366"},{"id":"R1450","pred":"compound","subj":"T2374","obj":"T2373"},{"id":"R1451","pred":"punct","subj":"T2375","obj":"T2373"},{"id":"R1452","pred":"nummod","subj":"T2376","obj":"T2377"},{"id":"R1453","pred":"npadvmod","subj":"T2377","obj":"T2379"},{"id":"R1454","pred":"amod","subj":"T2378","obj":"T2377"},{"id":"R1455","pred":"amod","subj":"T2379","obj":"T2373"},{"id":"R1456","pred":"prep","subj":"T2380","obj":"T2379"},{"id":"R1457","pred":"det","subj":"T2381","obj":"T2382"},{"id":"R1458","pred":"pobj","subj":"T2382","obj":"T2380"},{"id":"R1459","pred":"acl","subj":"T2383","obj":"T2382"},{"id":"R1460","pred":"agent","subj":"T2384","obj":"T2383"},{"id":"R1461","pred":"det","subj":"T2385","obj":"T2386"},{"id":"R1462","pred":"pobj","subj":"T2386","obj":"T2384"},{"id":"R1463","pred":"nummod","subj":"T2387","obj":"T2388"},{"id":"R1464","pred":"compound","subj":"T2388","obj":"T2386"},{"id":"R1465","pred":"punct","subj":"T2389","obj":"T2366"}],"text":"In order to determine the sequence of the entire transcript, RT-PCR using primers in exons 10 and 11 paired with a primer in intron 12 was performed using BALB/c mouse brain total RNA and the resulting products sequenced. These products show that the larger transcript is due to an alternative splice event that results in an expanded exon 13. Specifically, exon 12 splices at bp 436 of intron 12, creating a large 1614 bp exon 13 that splices correctly to exon 14. The open reading frame of this alternatively spliced transcript is 611 amino acids, 28 amino acids longer than the message encoded by the 2.4 kb transcript."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T2038","span":{"begin":49,"end":59},"obj":"SO:0000673"},{"id":"T2039","span":{"begin":74,"end":81},"obj":"SO:0000112"},{"id":"T2040","span":{"begin":85,"end":90},"obj":"SO:0000147"},{"id":"T2041","span":{"begin":115,"end":121},"obj":"SO:0000112"},{"id":"T2042","span":{"begin":125,"end":131},"obj":"SO:0000188"},{"id":"T2043","span":{"begin":162,"end":167},"obj":"NCBITaxon:10088"},{"id":"T2044","span":{"begin":168,"end":173},"obj":"UBERON:0000955"},{"id":"T2045","span":{"begin":258,"end":268},"obj":"SO:0000673"},{"id":"T2046","span":{"begin":282,"end":300},"obj":"GO:0000380"},{"id":"T2047","span":{"begin":335,"end":339},"obj":"SO:0000147"},{"id":"T2048","span":{"begin":358,"end":362},"obj":"SO:0000147"},{"id":"T2049","span":{"begin":366,"end":373},"obj":"GO:0008380"},{"id":"T2050","span":{"begin":377,"end":379},"obj":"SO:0000028"},{"id":"T2051","span":{"begin":387,"end":393},"obj":"SO:0000188"},{"id":"T2052","span":{"begin":420,"end":422},"obj":"SO:0000028"},{"id":"T2053","span":{"begin":423,"end":427},"obj":"SO:0000147"},{"id":"T2054","span":{"begin":436,"end":443},"obj":"GO:0008380"},{"id":"T2055","span":{"begin":457,"end":461},"obj":"SO:0000147"},{"id":"T2056","span":{"begin":470,"end":488},"obj":"SO:0000236"},{"id":"T2057","span":{"begin":497,"end":518},"obj":"GO:0000380"},{"id":"T2058","span":{"begin":497,"end":529},"obj":"SO:1001187"},{"id":"T2059","span":{"begin":581,"end":588},"obj":"CHEBI:33695"},{"id":"T2060","span":{"begin":611,"end":621},"obj":"SO:0000673"}],"text":"In order to determine the sequence of the entire transcript, RT-PCR using primers in exons 10 and 11 paired with a primer in intron 12 was performed using BALB/c mouse brain total RNA and the resulting products sequenced. These products show that the larger transcript is due to an alternative splice event that results in an expanded exon 13. Specifically, exon 12 splices at bp 436 of intron 12, creating a large 1614 bp exon 13 that splices correctly to exon 14. The open reading frame of this alternatively spliced transcript is 611 amino acids, 28 amino acids longer than the message encoded by the 2.4 kb transcript."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T2756","span":{"begin":26,"end":34},"obj":"SO_EXT:biological_sequence"},{"id":"T2757","span":{"begin":49,"end":59},"obj":"SO_EXT:0000673"},{"id":"T2758","span":{"begin":61,"end":63},"obj":"GO_EXT:reverse_transcription_or_reverse_transcriptase"},{"id":"T2759","span":{"begin":74,"end":81},"obj":"SO_EXT:0000112"},{"id":"T2760","span":{"begin":85,"end":90},"obj":"SO_EXT:0000147"},{"id":"T2761","span":{"begin":115,"end":121},"obj":"SO_EXT:0000112"},{"id":"T2762","span":{"begin":125,"end":131},"obj":"SO_EXT:0000188"},{"id":"T2763","span":{"begin":162,"end":167},"obj":"NCBITaxon:10088"},{"id":"T2764","span":{"begin":168,"end":173},"obj":"UBERON:0000955"},{"id":"T2765","span":{"begin":180,"end":183},"obj":"CHEBI_SO_EXT:RNA"},{"id":"T2766","span":{"begin":258,"end":268},"obj":"SO_EXT:0000673"},{"id":"T2767","span":{"begin":282,"end":300},"obj":"GO:0000380"},{"id":"T2768","span":{"begin":335,"end":339},"obj":"SO_EXT:0000147"},{"id":"T2769","span":{"begin":358,"end":362},"obj":"SO_EXT:0000147"},{"id":"T2770","span":{"begin":366,"end":373},"obj":"GO:0008380"},{"id":"T2771","span":{"begin":377,"end":379},"obj":"SO_EXT:0000028"},{"id":"T2772","span":{"begin":387,"end":393},"obj":"SO_EXT:0000188"},{"id":"T2773","span":{"begin":420,"end":422},"obj":"SO_EXT:0000028"},{"id":"T2774","span":{"begin":423,"end":427},"obj":"SO_EXT:0000147"},{"id":"T2775","span":{"begin":436,"end":443},"obj":"GO:0008380"},{"id":"T2776","span":{"begin":457,"end":461},"obj":"SO_EXT:0000147"},{"id":"T2777","span":{"begin":470,"end":488},"obj":"SO_EXT:0000236"},{"id":"T2778","span":{"begin":497,"end":518},"obj":"GO:0000380"},{"id":"T2779","span":{"begin":497,"end":529},"obj":"SO_EXT:1001187"},{"id":"T2780","span":{"begin":537,"end":548},"obj":"CHEBI_SO_EXT:amino_acid"},{"id":"T2781","span":{"begin":553,"end":564},"obj":"CHEBI_SO_EXT:amino_acid"},{"id":"T2782","span":{"begin":581,"end":588},"obj":"CHEBI_SO_EXT:mRNA"},{"id":"T2783","span":{"begin":581,"end":588},"obj":"CHEBI:33695"},{"id":"T2784","span":{"begin":589,"end":596},"obj":"SO_EXT:sequence_coding_function"},{"id":"T2785","span":{"begin":609,"end":610},"obj":"CHEBI_SO_EXT:base"},{"id":"T2786","span":{"begin":611,"end":621},"obj":"SO_EXT:0000673"}],"text":"In order to determine the sequence of the entire transcript, RT-PCR using primers in exons 10 and 11 paired with a primer in intron 12 was performed using BALB/c mouse brain total RNA and the resulting products sequenced. These products show that the larger transcript is due to an alternative splice event that results in an expanded exon 13. Specifically, exon 12 splices at bp 436 of intron 12, creating a large 1614 bp exon 13 that splices correctly to exon 14. The open reading frame of this alternatively spliced transcript is 611 amino acids, 28 amino acids longer than the message encoded by the 2.4 kb transcript."}