Id |
Subject |
Object |
Predicate |
Lexical cue |
T2267 |
0-2 |
IN |
denotes |
In |
T2268 |
139-148 |
VBN |
denotes |
performed |
T2269 |
3-8 |
NN |
denotes |
order |
T2270 |
9-11 |
TO |
denotes |
to |
T2271 |
12-21 |
VB |
denotes |
determine |
T2272 |
22-25 |
DT |
denotes |
the |
T2273 |
26-34 |
NN |
denotes |
sequence |
T2274 |
35-37 |
IN |
denotes |
of |
T2275 |
38-41 |
DT |
denotes |
the |
T2276 |
49-59 |
NN |
denotes |
transcript |
T2277 |
42-48 |
JJ |
denotes |
entire |
T2278 |
59-61 |
, |
denotes |
, |
T2279 |
61-63 |
NN |
denotes |
RT |
T2280 |
64-67 |
NN |
denotes |
PCR |
T2281 |
63-64 |
HYPH |
denotes |
- |
T2282 |
68-73 |
VBG |
denotes |
using |
T2283 |
74-81 |
NNS |
denotes |
primers |
T2284 |
82-84 |
IN |
denotes |
in |
T2285 |
85-90 |
NNS |
denotes |
exons |
T2286 |
91-93 |
CD |
denotes |
10 |
T2287 |
94-97 |
CC |
denotes |
and |
T2288 |
98-100 |
CD |
denotes |
11 |
T2289 |
101-107 |
VBN |
denotes |
paired |
T2290 |
108-112 |
IN |
denotes |
with |
T2291 |
113-114 |
DT |
denotes |
a |
T2292 |
115-121 |
NN |
denotes |
primer |
T2293 |
122-124 |
IN |
denotes |
in |
T2294 |
125-131 |
NN |
denotes |
intron |
T2295 |
132-134 |
CD |
denotes |
12 |
T2296 |
135-138 |
VBD |
denotes |
was |
T2297 |
149-154 |
VBG |
denotes |
using |
T2298 |
155-159 |
NN |
denotes |
BALB |
T2299 |
160-161 |
NN |
denotes |
c |
T2300 |
159-160 |
HYPH |
denotes |
/ |
T2301 |
162-167 |
NN |
denotes |
mouse |
T2302 |
168-173 |
NN |
denotes |
brain |
T2303 |
180-183 |
NN |
denotes |
RNA |
T2304 |
174-179 |
JJ |
denotes |
total |
T2305 |
184-187 |
CC |
denotes |
and |
T2306 |
188-191 |
DT |
denotes |
the |
T2307 |
202-210 |
NNS |
denotes |
products |
T2308 |
192-201 |
VBG |
denotes |
resulting |
T2309 |
211-220 |
VBN |
denotes |
sequenced |
T2310 |
220-221 |
. |
denotes |
. |
T2311 |
221-343 |
sentence |
denotes |
These products show that the larger transcript is due to an alternative splice event that results in an expanded exon 13. |
T2312 |
222-227 |
DT |
denotes |
These |
T2313 |
228-236 |
NNS |
denotes |
products |
T2314 |
237-241 |
VBP |
denotes |
show |
T2315 |
242-246 |
IN |
denotes |
that |
T2316 |
269-271 |
VBZ |
denotes |
is |
T2317 |
247-250 |
DT |
denotes |
the |
T2318 |
258-268 |
NN |
denotes |
transcript |
T2319 |
251-257 |
JJR |
denotes |
larger |
T2320 |
272-275 |
IN |
denotes |
due |
T2321 |
276-278 |
IN |
denotes |
to |
T2322 |
279-281 |
DT |
denotes |
an |
T2323 |
301-306 |
NN |
denotes |
event |
T2324 |
282-293 |
JJ |
denotes |
alternative |
T2325 |
294-300 |
NN |
denotes |
splice |
T2326 |
307-311 |
WDT |
denotes |
that |
T2327 |
312-319 |
VBZ |
denotes |
results |
T2328 |
320-322 |
IN |
denotes |
in |
T2329 |
323-325 |
DT |
denotes |
an |
T2330 |
335-339 |
NN |
denotes |
exon |
T2331 |
326-334 |
VBN |
denotes |
expanded |
T2332 |
340-342 |
CD |
denotes |
13 |
T2333 |
342-343 |
. |
denotes |
. |
T2334 |
343-465 |
sentence |
denotes |
Specifically, exon 12 splices at bp 436 of intron 12, creating a large 1614 bp exon 13 that splices correctly to exon 14. |
T2335 |
344-356 |
RB |
denotes |
Specifically |
T2336 |
366-373 |
VBZ |
denotes |
splices |
T2337 |
356-358 |
, |
denotes |
, |
T2338 |
358-362 |
NN |
denotes |
exon |
T2339 |
363-365 |
CD |
denotes |
12 |
T2340 |
374-376 |
IN |
denotes |
at |
T2341 |
377-379 |
NN |
denotes |
bp |
T2342 |
380-383 |
CD |
denotes |
436 |
T2343 |
384-386 |
IN |
denotes |
of |
T2344 |
387-393 |
NN |
denotes |
intron |
T2345 |
394-396 |
CD |
denotes |
12 |
T2346 |
396-398 |
, |
denotes |
, |
T2347 |
398-406 |
VBG |
denotes |
creating |
T2348 |
407-408 |
DT |
denotes |
a |
T2349 |
423-427 |
NN |
denotes |
exon |
T2350 |
409-414 |
JJ |
denotes |
large |
T2351 |
415-419 |
CD |
denotes |
1614 |
T2352 |
420-422 |
NN |
denotes |
bp |
T2353 |
428-430 |
CD |
denotes |
13 |
T2354 |
431-435 |
WDT |
denotes |
that |
T2355 |
436-443 |
VBZ |
denotes |
splices |
T2356 |
444-453 |
RB |
denotes |
correctly |
T2357 |
454-456 |
IN |
denotes |
to |
T2358 |
457-461 |
NN |
denotes |
exon |
T2359 |
462-464 |
CD |
denotes |
14 |
T2360 |
464-465 |
. |
denotes |
. |
T2361 |
465-622 |
sentence |
denotes |
The open reading frame of this alternatively spliced transcript is 611 amino acids, 28 amino acids longer than the message encoded by the 2.4 kb transcript. |
T2362 |
466-469 |
DT |
denotes |
The |
T2363 |
483-488 |
NN |
denotes |
frame |
T2364 |
470-474 |
JJ |
denotes |
open |
T2365 |
475-482 |
NN |
denotes |
reading |
T2366 |
530-532 |
VBZ |
denotes |
is |
T2367 |
489-491 |
IN |
denotes |
of |
T2368 |
492-496 |
DT |
denotes |
this |
T2369 |
519-529 |
NN |
denotes |
transcript |
T2370 |
497-510 |
RB |
denotes |
alternatively |
T2371 |
511-518 |
VBN |
denotes |
spliced |
T2372 |
533-536 |
CD |
denotes |
611 |
T2373 |
543-548 |
NNS |
denotes |
acids |
T2374 |
537-542 |
NN |
denotes |
amino |
T2375 |
548-550 |
, |
denotes |
, |
T2376 |
550-552 |
CD |
denotes |
28 |
T2377 |
559-564 |
NNS |
denotes |
acids |
T2378 |
553-558 |
JJ |
denotes |
amino |
T2379 |
565-571 |
JJR |
denotes |
longer |
T2380 |
572-576 |
IN |
denotes |
than |
T2381 |
577-580 |
DT |
denotes |
the |
T2382 |
581-588 |
NN |
denotes |
message |
T2383 |
589-596 |
VBN |
denotes |
encoded |
T2384 |
597-599 |
IN |
denotes |
by |
T2385 |
600-603 |
DT |
denotes |
the |
T2386 |
611-621 |
NN |
denotes |
transcript |
T2387 |
604-607 |
CD |
denotes |
2.4 |
T2388 |
608-610 |
NN |
denotes |
kb |
T2389 |
621-622 |
. |
denotes |
. |
R1350 |
T2267 |
T2268 |
prep |
In,performed |
R1351 |
T2269 |
T2267 |
pobj |
order,In |
R1352 |
T2270 |
T2271 |
aux |
to,determine |
R1353 |
T2271 |
T2269 |
acl |
determine,order |
R1354 |
T2272 |
T2273 |
det |
the,sequence |
R1355 |
T2273 |
T2271 |
dobj |
sequence,determine |
R1356 |
T2274 |
T2273 |
prep |
of,sequence |
R1357 |
T2275 |
T2276 |
det |
the,transcript |
R1358 |
T2276 |
T2274 |
pobj |
transcript,of |
R1359 |
T2277 |
T2276 |
amod |
entire,transcript |
R1360 |
T2278 |
T2268 |
punct |
", ",performed |
R1361 |
T2279 |
T2280 |
compound |
RT,PCR |
R1362 |
T2280 |
T2268 |
nsubjpass |
PCR,performed |
R1363 |
T2281 |
T2280 |
punct |
-,PCR |
R1364 |
T2282 |
T2280 |
acl |
using,PCR |
R1365 |
T2283 |
T2282 |
dobj |
primers,using |
R1366 |
T2284 |
T2283 |
prep |
in,primers |
R1367 |
T2285 |
T2286 |
nmod |
exons,10 |
R1368 |
T2286 |
T2284 |
pobj |
10,in |
R1369 |
T2287 |
T2286 |
cc |
and,10 |
R1370 |
T2288 |
T2286 |
conj |
11,10 |
R1371 |
T2289 |
T2283 |
acl |
paired,primers |
R1372 |
T2290 |
T2289 |
prep |
with,paired |
R1373 |
T2291 |
T2292 |
det |
a,primer |
R1374 |
T2292 |
T2290 |
pobj |
primer,with |
R1375 |
T2293 |
T2292 |
prep |
in,primer |
R1376 |
T2294 |
T2293 |
pobj |
intron,in |
R1377 |
T2295 |
T2294 |
nummod |
12,intron |
R1378 |
T2296 |
T2268 |
auxpass |
was,performed |
R1379 |
T2297 |
T2268 |
advcl |
using,performed |
R1380 |
T2298 |
T2299 |
nmod |
BALB,c |
R1381 |
T2299 |
T2301 |
nmod |
c,mouse |
R1382 |
T2300 |
T2299 |
punct |
/,c |
R1383 |
T2301 |
T2302 |
nmod |
mouse,brain |
R1384 |
T2302 |
T2303 |
nmod |
brain,RNA |
R1385 |
T2303 |
T2297 |
dobj |
RNA,using |
R1386 |
T2304 |
T2303 |
amod |
total,RNA |
R1387 |
T2305 |
T2268 |
cc |
and,performed |
R1388 |
T2306 |
T2307 |
det |
the,products |
R1389 |
T2307 |
T2309 |
nsubj |
products,sequenced |
R1390 |
T2308 |
T2307 |
amod |
resulting,products |
R1391 |
T2309 |
T2268 |
conj |
sequenced,performed |
R1392 |
T2310 |
T2268 |
punct |
.,performed |
R1393 |
T2312 |
T2313 |
det |
These,products |
R1394 |
T2313 |
T2314 |
nsubj |
products,show |
R1395 |
T2315 |
T2316 |
mark |
that,is |
R1396 |
T2316 |
T2314 |
ccomp |
is,show |
R1397 |
T2317 |
T2318 |
det |
the,transcript |
R1398 |
T2318 |
T2316 |
nsubj |
transcript,is |
R1399 |
T2319 |
T2318 |
amod |
larger,transcript |
R1400 |
T2320 |
T2316 |
prep |
due,is |
R1401 |
T2321 |
T2320 |
pcomp |
to,due |
R1402 |
T2322 |
T2323 |
det |
an,event |
R1403 |
T2323 |
T2320 |
pobj |
event,due |
R1404 |
T2324 |
T2323 |
amod |
alternative,event |
R1405 |
T2325 |
T2323 |
compound |
splice,event |
R1406 |
T2326 |
T2327 |
dep |
that,results |
R1407 |
T2327 |
T2323 |
relcl |
results,event |
R1408 |
T2328 |
T2327 |
prep |
in,results |
R1409 |
T2329 |
T2330 |
det |
an,exon |
R1410 |
T2330 |
T2328 |
pobj |
exon,in |
R1411 |
T2331 |
T2330 |
amod |
expanded,exon |
R1412 |
T2332 |
T2330 |
nummod |
13,exon |
R1413 |
T2333 |
T2314 |
punct |
.,show |
R1414 |
T2335 |
T2336 |
advmod |
Specifically,splices |
R1415 |
T2337 |
T2336 |
punct |
", ",splices |
R1416 |
T2338 |
T2336 |
nsubj |
exon,splices |
R1417 |
T2339 |
T2338 |
nummod |
12,exon |
R1418 |
T2340 |
T2336 |
prep |
at,splices |
R1419 |
T2341 |
T2340 |
pobj |
bp,at |
R1420 |
T2342 |
T2341 |
nummod |
436,bp |
R1421 |
T2343 |
T2341 |
prep |
of,bp |
R1422 |
T2344 |
T2343 |
pobj |
intron,of |
R1423 |
T2345 |
T2344 |
nummod |
12,intron |
R1424 |
T2346 |
T2336 |
punct |
", ",splices |
R1425 |
T2347 |
T2336 |
advcl |
creating,splices |
R1426 |
T2348 |
T2349 |
det |
a,exon |
R1427 |
T2349 |
T2347 |
dobj |
exon,creating |
R1428 |
T2350 |
T2349 |
amod |
large,exon |
R1429 |
T2351 |
T2352 |
nummod |
1614,bp |
R1430 |
T2352 |
T2349 |
compound |
bp,exon |
R1431 |
T2353 |
T2349 |
nummod |
13,exon |
R1432 |
T2354 |
T2355 |
dep |
that,splices |
R1433 |
T2355 |
T2349 |
relcl |
splices,exon |
R1434 |
T2356 |
T2355 |
advmod |
correctly,splices |
R1435 |
T2357 |
T2355 |
prep |
to,splices |
R1436 |
T2358 |
T2357 |
pobj |
exon,to |
R1437 |
T2359 |
T2358 |
nummod |
14,exon |
R1438 |
T2360 |
T2336 |
punct |
.,splices |
R1439 |
T2362 |
T2363 |
det |
The,frame |
R1440 |
T2363 |
T2366 |
nsubj |
frame,is |
R1441 |
T2364 |
T2363 |
amod |
open,frame |
R1442 |
T2365 |
T2363 |
compound |
reading,frame |
R1443 |
T2367 |
T2363 |
prep |
of,frame |
R1444 |
T2368 |
T2369 |
det |
this,transcript |
R1445 |
T2369 |
T2367 |
pobj |
transcript,of |
R1446 |
T2370 |
T2371 |
advmod |
alternatively,spliced |
R1447 |
T2371 |
T2369 |
amod |
spliced,transcript |
R1448 |
T2372 |
T2373 |
nummod |
611,acids |
R1449 |
T2373 |
T2366 |
attr |
acids,is |
R1450 |
T2374 |
T2373 |
compound |
amino,acids |
R1451 |
T2375 |
T2373 |
punct |
", ",acids |
R1452 |
T2376 |
T2377 |
nummod |
28,acids |
R1453 |
T2377 |
T2379 |
npadvmod |
acids,longer |
R1454 |
T2378 |
T2377 |
amod |
amino,acids |
R1455 |
T2379 |
T2373 |
amod |
longer,acids |
R1456 |
T2380 |
T2379 |
prep |
than,longer |
R1457 |
T2381 |
T2382 |
det |
the,message |
R1458 |
T2382 |
T2380 |
pobj |
message,than |
R1459 |
T2383 |
T2382 |
acl |
encoded,message |
R1460 |
T2384 |
T2383 |
agent |
by,encoded |
R1461 |
T2385 |
T2386 |
det |
the,transcript |
R1462 |
T2386 |
T2384 |
pobj |
transcript,by |
R1463 |
T2387 |
T2388 |
nummod |
2.4,kb |
R1464 |
T2388 |
T2386 |
compound |
kb,transcript |
R1465 |
T2389 |
T2366 |
punct |
.,is |