PMC:7795856 / 8363-9075 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"195","span":{"begin":352,"end":355},"obj":"Gene"},{"id":"196","span":{"begin":458,"end":480},"obj":"Chemical"},{"id":"197","span":{"begin":481,"end":495},"obj":"Chemical"},{"id":"198","span":{"begin":517,"end":520},"obj":"Chemical"},{"id":"199","span":{"begin":616,"end":619},"obj":"Chemical"}],"attributes":[{"id":"A195","pred":"tao:has_database_id","subj":"195","obj":"Gene:134864"},{"id":"A196","pred":"tao:has_database_id","subj":"196","obj":"MESH:D012967"},{"id":"A197","pred":"tao:has_database_id","subj":"197","obj":"MESH:C016679"},{"id":"A198","pred":"tao:has_database_id","subj":"198","obj":"MESH:D012967"},{"id":"A199","pred":"tao:has_database_id","subj":"199","obj":"MESH:D012967"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"The whole cell lysate was analyzed prior to and 15 h after induction by Western blotting. Using a monoclonal antibody that recognizes an epitope of the PAS#1 sequence, a distinct band with an approximate molecular size above 250 kDa was detected (Figure 1b), which demonstrated successful bacterial expression of the full-length C-terminally PASylated Tα1 (Tα1-PAS) without signs of degradation. Of note, the unusually slow migration of Tα1-PAS (52.7 kD) in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is well known for PASylated proteins [31,37] and can be explained by the poor binding of SDS (which provides the electrophoretic driving force) to the strongly hydrophilic PAS sequence."}

{"project":"LitCovid-sentences","denotations":[{"id":"T53","span":{"begin":0,"end":89},"obj":"Sentence"},{"id":"T54","span":{"begin":90,"end":395},"obj":"Sentence"},{"id":"T55","span":{"begin":396,"end":712},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The whole cell lysate was analyzed prior to and 15 h after induction by Western blotting. Using a monoclonal antibody that recognizes an epitope of the PAS#1 sequence, a distinct band with an approximate molecular size above 250 kDa was detected (Figure 1b), which demonstrated successful bacterial expression of the full-length C-terminally PASylated Tα1 (Tα1-PAS) without signs of degradation. Of note, the unusually slow migration of Tα1-PAS (52.7 kD) in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is well known for PASylated proteins [31,37] and can be explained by the poor binding of SDS (which provides the electrophoretic driving force) to the strongly hydrophilic PAS sequence."}