| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T53 |
0-89 |
Sentence |
denotes |
The whole cell lysate was analyzed prior to and 15 h after induction by Western blotting. |
| T54 |
90-395 |
Sentence |
denotes |
Using a monoclonal antibody that recognizes an epitope of the PAS#1 sequence, a distinct band with an approximate molecular size above 250 kDa was detected (Figure 1b), which demonstrated successful bacterial expression of the full-length C-terminally PASylated Tα1 (Tα1-PAS) without signs of degradation. |
| T55 |
396-712 |
Sentence |
denotes |
Of note, the unusually slow migration of Tα1-PAS (52.7 kD) in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is well known for PASylated proteins [31,37] and can be explained by the poor binding of SDS (which provides the electrophoretic driving force) to the strongly hydrophilic PAS sequence. |
