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PMC:7795856 / 7597-8362
Annnotations
LitCovid-PubTator
{"project":"LitCovid-PubTator","denotations":[{"id":"183","span":{"begin":60,"end":63},"obj":"Gene"},{"id":"184","span":{"begin":168,"end":171},"obj":"Gene"},{"id":"185","span":{"begin":462,"end":465},"obj":"Gene"},{"id":"186","span":{"begin":162,"end":167},"obj":"Species"},{"id":"187","span":{"begin":294,"end":301},"obj":"Species"},{"id":"188","span":{"begin":670,"end":677},"obj":"Species"},{"id":"189","span":{"begin":738,"end":741},"obj":"Chemical"}],"attributes":[{"id":"A183","pred":"tao:has_database_id","subj":"183","obj":"Gene:134864"},{"id":"A184","pred":"tao:has_database_id","subj":"184","obj":"Gene:134864"},{"id":"A185","pred":"tao:has_database_id","subj":"185","obj":"Gene:134864"},{"id":"A186","pred":"tao:has_database_id","subj":"186","obj":"Tax:9606"},{"id":"A187","pred":"tao:has_database_id","subj":"187","obj":"Tax:562"},{"id":"A188","pred":"tao:has_database_id","subj":"188","obj":"Tax:562"},{"id":"A189","pred":"tao:has_database_id","subj":"189","obj":"MESH:C010349"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"To achieve C-terminal PASylation of N-terminally acetylated Tα1, a plasmid harboring a bicistronic operon was constructed to allow the simultaneous expression of human Tα1 (UniProtKB ID: P06454; residues 2–29), C-terminally fused with a PAS polypeptide comprising 601 amino acids [34], and the E. coli N-acetyltransferase RimJ (UniProtKB ID: P0A948) based on the vector pASK75 (Figure 1) [36]. In a parallel attempt, a plasmid encoding an N-terminally PASylated Tα1 was constructed, again, using plasmid pASK75 as the backbone (this time omitting the RimJ cistron, see below). Cytoplasmic gene expression was performed in both cases on a 2 L shake flask scale using the E. coli strain NEBexpress under control of the chemically inducible tet promoter/operator [36]."}
LitCovid-sentences
{"project":"LitCovid-sentences","denotations":[{"id":"T50","span":{"begin":0,"end":393},"obj":"Sentence"},{"id":"T51","span":{"begin":394,"end":576},"obj":"Sentence"},{"id":"T52","span":{"begin":577,"end":765},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"To achieve C-terminal PASylation of N-terminally acetylated Tα1, a plasmid harboring a bicistronic operon was constructed to allow the simultaneous expression of human Tα1 (UniProtKB ID: P06454; residues 2–29), C-terminally fused with a PAS polypeptide comprising 601 amino acids [34], and the E. coli N-acetyltransferase RimJ (UniProtKB ID: P0A948) based on the vector pASK75 (Figure 1) [36]. In a parallel attempt, a plasmid encoding an N-terminally PASylated Tα1 was constructed, again, using plasmid pASK75 as the backbone (this time omitting the RimJ cistron, see below). Cytoplasmic gene expression was performed in both cases on a 2 L shake flask scale using the E. coli strain NEBexpress under control of the chemically inducible tet promoter/operator [36]."}