PMC:7795856 / 7597-8362 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue	tao:has_database_id
183	60-63	Gene	denotes	Tα1	Gene:134864
184	168-171	Gene	denotes	Tα1	Gene:134864
185	462-465	Gene	denotes	Tα1	Gene:134864
186	162-167	Species	denotes	human	Tax:9606
187	294-301	Species	denotes	E. coli	Tax:562
188	670-677	Species	denotes	E. coli	Tax:562
189	738-741	Chemical	denotes	tet	MESH:C010349

Id	Subject	Object	Predicate	Lexical cue
T50	0-393	Sentence	denotes	To achieve C-terminal PASylation of N-terminally acetylated Tα1, a plasmid harboring a bicistronic operon was constructed to allow the simultaneous expression of human Tα1 (UniProtKB ID: P06454; residues 2–29), C-terminally fused with a PAS polypeptide comprising 601 amino acids [34], and the E. coli N-acetyltransferase RimJ (UniProtKB ID: P0A948) based on the vector pASK75 (Figure 1) [36].
T51	394-576	Sentence	denotes	In a parallel attempt, a plasmid encoding an N-terminally PASylated Tα1 was constructed, again, using plasmid pASK75 as the backbone (this time omitting the RimJ cistron, see below).
T52	577-765	Sentence	denotes	Cytoplasmic gene expression was performed in both cases on a 2 L shake flask scale using the E. coli strain NEBexpress under control of the chemically inducible tet promoter/operator [36].