PMC:7795856 / 39515-40528 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"844","span":{"begin":66,"end":69},"obj":"Gene"},{"id":"845","span":{"begin":200,"end":203},"obj":"Gene"},{"id":"846","span":{"begin":375,"end":378},"obj":"Gene"},{"id":"847","span":{"begin":453,"end":456},"obj":"Gene"},{"id":"848","span":{"begin":618,"end":621},"obj":"Gene"},{"id":"849","span":{"begin":744,"end":747},"obj":"Gene"},{"id":"850","span":{"begin":788,"end":791},"obj":"Gene"},{"id":"851","span":{"begin":369,"end":374},"obj":"Species"},{"id":"852","span":{"begin":475,"end":482},"obj":"Species"},{"id":"853","span":{"begin":333,"end":343},"obj":"Chemical"},{"id":"854","span":{"begin":350,"end":357},"obj":"Chemical"},{"id":"855","span":{"begin":651,"end":661},"obj":"Chemical"}],"attributes":[{"id":"A844","pred":"tao:has_database_id","subj":"844","obj":"Gene:134864"},{"id":"A845","pred":"tao:has_database_id","subj":"845","obj":"Gene:134864"},{"id":"A846","pred":"tao:has_database_id","subj":"846","obj":"Gene:134864"},{"id":"A847","pred":"tao:has_database_id","subj":"847","obj":"Gene:134864"},{"id":"A848","pred":"tao:has_database_id","subj":"848","obj":"Gene:134864"},{"id":"A849","pred":"tao:has_database_id","subj":"849","obj":"Gene:134864"},{"id":"A850","pred":"tao:has_database_id","subj":"850","obj":"Gene:134864"},{"id":"A851","pred":"tao:has_database_id","subj":"851","obj":"Tax:9606"},{"id":"A852","pred":"tao:has_database_id","subj":"852","obj":"Tax:562"},{"id":"A853","pred":"tao:has_database_id","subj":"853","obj":"MESH:D008715"},{"id":"A854","pred":"tao:has_database_id","subj":"854","obj":"MESH:D011392"},{"id":"A855","pred":"tao:has_database_id","subj":"855","obj":"MESH:D008715"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Figure 3 Preparation of an N-terminally PASylated version of the Tα1 peptide and analysis of its integrity, purity, and hydrodynamic volume. (a) Expression vector for the bacterial production of PAS-Tα1. The gene cassette cloned on pASK75 comprises the T7 RBS, the coding region for a 601-residue PAS#1 sequence preceded by a start methionine and a proline codon, the human Tα1 sequence, and, finally, tlpp. (b) The deconvoluted ESI-MS spectrum of PAS-Tα1 purified from the E. coli cell extract by subtractive AEX and CEX (see text) reveals a molecular mass of 52,789.69 Da, which matches the calculated mass for PAS-Tα1 with a fully processed start methionine (52,789.66 Da). (c) Reverse-phase chromatography indicates a purity \u003e 96% for PAS-Tα1. (d) Analytical SEC of the purified PAS-Tα1 in PBS results in a single peak with an elution volume of 13.2 mL. Comparison with a half-logarithmic calibration curve (inset) indicates a strongly expanded hydrodynamic volume with an apparent molecular size of 665 kDa."}

{"project":"LitCovid-sentences","denotations":[{"id":"T300","span":{"begin":0,"end":204},"obj":"Sentence"},{"id":"T301","span":{"begin":205,"end":858},"obj":"Sentence"},{"id":"T302","span":{"begin":859,"end":1013},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Figure 3 Preparation of an N-terminally PASylated version of the Tα1 peptide and analysis of its integrity, purity, and hydrodynamic volume. (a) Expression vector for the bacterial production of PAS-Tα1. The gene cassette cloned on pASK75 comprises the T7 RBS, the coding region for a 601-residue PAS#1 sequence preceded by a start methionine and a proline codon, the human Tα1 sequence, and, finally, tlpp. (b) The deconvoluted ESI-MS spectrum of PAS-Tα1 purified from the E. coli cell extract by subtractive AEX and CEX (see text) reveals a molecular mass of 52,789.69 Da, which matches the calculated mass for PAS-Tα1 with a fully processed start methionine (52,789.66 Da). (c) Reverse-phase chromatography indicates a purity \u003e 96% for PAS-Tα1. (d) Analytical SEC of the purified PAS-Tα1 in PBS results in a single peak with an elution volume of 13.2 mL. Comparison with a half-logarithmic calibration curve (inset) indicates a strongly expanded hydrodynamic volume with an apparent molecular size of 665 kDa."}