PMC:7795856 / 39515-40528 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue	tao:has_database_id
844	66-69	Gene	denotes	Tα1	Gene:134864
845	200-203	Gene	denotes	Tα1	Gene:134864
846	375-378	Gene	denotes	Tα1	Gene:134864
847	453-456	Gene	denotes	Tα1	Gene:134864
848	618-621	Gene	denotes	Tα1	Gene:134864
849	744-747	Gene	denotes	Tα1	Gene:134864
850	788-791	Gene	denotes	Tα1	Gene:134864
851	369-374	Species	denotes	human	Tax:9606
852	475-482	Species	denotes	E. coli	Tax:562
853	333-343	Chemical	denotes	methionine	MESH:D008715
854	350-357	Chemical	denotes	proline	MESH:D011392
855	651-661	Chemical	denotes	methionine	MESH:D008715

Id	Subject	Object	Predicate	Lexical cue
T300	0-204	Sentence	denotes	Figure 3 Preparation of an N-terminally PASylated version of the Tα1 peptide and analysis of its integrity, purity, and hydrodynamic volume. (a) Expression vector for the bacterial production of PAS-Tα1.
T301	205-858	Sentence	denotes	The gene cassette cloned on pASK75 comprises the T7 RBS, the coding region for a 601-residue PAS#1 sequence preceded by a start methionine and a proline codon, the human Tα1 sequence, and, finally, tlpp. (b) The deconvoluted ESI-MS spectrum of PAS-Tα1 purified from the E. coli cell extract by subtractive AEX and CEX (see text) reveals a molecular mass of 52,789.69 Da, which matches the calculated mass for PAS-Tα1 with a fully processed start methionine (52,789.66 Da). (c) Reverse-phase chromatography indicates a purity > 96% for PAS-Tα1. (d) Analytical SEC of the purified PAS-Tα1 in PBS results in a single peak with an elution volume of 13.2 mL.
T302	859-1013	Sentence	denotes	Comparison with a half-logarithmic calibration curve (inset) indicates a strongly expanded hydrodynamic volume with an apparent molecular size of 665 kDa.