PMC:7795856 / 37847-38251 JSONTXT

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{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7795856","sourcedb":"PMC","sourceid":"7795856","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7795856","text":"The gene cassette cloned on pASK75 comprises the T7 phage ribosome binding site (T7 RBS) followed by the coding sequence for human Tα1 fused to a 601-residue PAS#1 sequence, the second RBS, the coding sequence for RimJ, and the lipoprotein terminator (tlpp). (b) Western blot analysis after 4–12% SDS–PAGE of Tα1-PAS in the E. coli whole cell lysate using an antibody directed against the PAS#1 sequence.","tracks":[{"project":"LitCovid-PubTator","denotations":[{"id":"812","span":{"begin":131,"end":134},"obj":"Gene"},{"id":"813","span":{"begin":125,"end":130},"obj":"Species"},{"id":"814","span":{"begin":324,"end":331},"obj":"Species"},{"id":"817","span":{"begin":297,"end":300},"obj":"Chemical"}],"attributes":[{"id":"A812","pred":"tao:has_database_id","subj":"812","obj":"Gene:134864"},{"id":"A813","pred":"tao:has_database_id","subj":"813","obj":"Tax:9606"},{"id":"A814","pred":"tao:has_database_id","subj":"814","obj":"Tax:562"},{"id":"A817","pred":"tao:has_database_id","subj":"817","obj":"MESH:D012967"},{"subj":"812","pred":"source","obj":"LitCovid-PubTator"},{"subj":"813","pred":"source","obj":"LitCovid-PubTator"},{"subj":"814","pred":"source","obj":"LitCovid-PubTator"},{"subj":"817","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T294","span":{"begin":0,"end":404},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T294","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PubTator","color":"#9cec93","default":true},{"id":"LitCovid-sentences","color":"#ec93b6"}]}]}}