PMC:7795856 / 37847-38251
Annnotations
LitCovid-PubTator
Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
---|---|---|---|---|---|
812 | 131-134 | Gene | denotes | Tα1 | Gene:134864 |
813 | 125-130 | Species | denotes | human | Tax:9606 |
814 | 324-331 | Species | denotes | E. coli | Tax:562 |
817 | 297-300 | Chemical | denotes | SDS | MESH:D012967 |
LitCovid-sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T294 | 0-404 | Sentence | denotes | The gene cassette cloned on pASK75 comprises the T7 phage ribosome binding site (T7 RBS) followed by the coding sequence for human Tα1 fused to a 601-residue PAS#1 sequence, the second RBS, the coding sequence for RimJ, and the lipoprotein terminator (tlpp). (b) Western blot analysis after 4–12% SDS–PAGE of Tα1-PAS in the E. coli whole cell lysate using an antibody directed against the PAS#1 sequence. |