PMC:7795856 / 37658-38576 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"812","span":{"begin":320,"end":323},"obj":"Gene"},{"id":"813","span":{"begin":314,"end":319},"obj":"Species"},{"id":"814","span":{"begin":513,"end":520},"obj":"Species"},{"id":"815","span":{"begin":24,"end":55},"obj":"Chemical"},{"id":"816","span":{"begin":181,"end":185},"obj":"Chemical"},{"id":"817","span":{"begin":486,"end":489},"obj":"Chemical"},{"id":"818","span":{"begin":722,"end":725},"obj":"Chemical"},{"id":"819","span":{"begin":800,"end":816},"obj":"Chemical"}],"attributes":[{"id":"A812","pred":"tao:has_database_id","subj":"812","obj":"Gene:134864"},{"id":"A813","pred":"tao:has_database_id","subj":"813","obj":"Tax:9606"},{"id":"A814","pred":"tao:has_database_id","subj":"814","obj":"Tax:562"},{"id":"A817","pred":"tao:has_database_id","subj":"817","obj":"MESH:D012967"},{"id":"A818","pred":"tao:has_database_id","subj":"818","obj":"MESH:D012967"},{"id":"A819","pred":"tao:has_database_id","subj":"819","obj":"MESH:D000645"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Bacterial production of N-terminally acetylated Tα1-PAS. (a) Expression vector for Tα1-PAS and the N-acetyltransferase RimJ from a bicistronic operon under control of the inducible tetp/o. The gene cassette cloned on pASK75 comprises the T7 phage ribosome binding site (T7 RBS) followed by the coding sequence for human Tα1 fused to a 601-residue PAS#1 sequence, the second RBS, the coding sequence for RimJ, and the lipoprotein terminator (tlpp). (b) Western blot analysis after 4–12% SDS–PAGE of Tα1-PAS in the E. coli whole cell lysate using an antibody directed against the PAS#1 sequence. M, protein size standard; lane 1, whole cell extract before induction; lane 2, whole cell extract 15 h after induction. (c) 10% SDS-PAGE of purified Tα1-PAS. M, protein size standard; lane 1, Tα1-PAS after ammonium sulfate precipitation, subtractive anion exchange (AEX), cation exchange (CEX), and final AEX chromatography."}

{"project":"LitCovid-sentences","denotations":[{"id":"T294","span":{"begin":189,"end":593},"obj":"Sentence"},{"id":"T295","span":{"begin":594,"end":751},"obj":"Sentence"},{"id":"T296","span":{"begin":752,"end":918},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Bacterial production of N-terminally acetylated Tα1-PAS. (a) Expression vector for Tα1-PAS and the N-acetyltransferase RimJ from a bicistronic operon under control of the inducible tetp/o. The gene cassette cloned on pASK75 comprises the T7 phage ribosome binding site (T7 RBS) followed by the coding sequence for human Tα1 fused to a 601-residue PAS#1 sequence, the second RBS, the coding sequence for RimJ, and the lipoprotein terminator (tlpp). (b) Western blot analysis after 4–12% SDS–PAGE of Tα1-PAS in the E. coli whole cell lysate using an antibody directed against the PAS#1 sequence. M, protein size standard; lane 1, whole cell extract before induction; lane 2, whole cell extract 15 h after induction. (c) 10% SDS-PAGE of purified Tα1-PAS. M, protein size standard; lane 1, Tα1-PAS after ammonium sulfate precipitation, subtractive anion exchange (AEX), cation exchange (CEX), and final AEX chromatography."}