PMC:7795856 / 37658-38576 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue	tao:has_database_id
812	320-323	Gene	denotes	Tα1	Gene:134864
813	314-319	Species	denotes	human	Tax:9606
814	513-520	Species	denotes	E. coli	Tax:562
815	24-55	Chemical	denotes	N-terminally acetylated Tα1-PAS
816	181-185	Chemical	denotes	tetp
817	486-489	Chemical	denotes	SDS	MESH:D012967
818	722-725	Chemical	denotes	SDS	MESH:D012967
819	800-816	Chemical	denotes	ammonium sulfate	MESH:D000645

Id	Subject	Object	Predicate	Lexical cue
T294	189-593	Sentence	denotes	The gene cassette cloned on pASK75 comprises the T7 phage ribosome binding site (T7 RBS) followed by the coding sequence for human Tα1 fused to a 601-residue PAS#1 sequence, the second RBS, the coding sequence for RimJ, and the lipoprotein terminator (tlpp). (b) Western blot analysis after 4–12% SDS–PAGE of Tα1-PAS in the E. coli whole cell lysate using an antibody directed against the PAS#1 sequence.
T295	594-751	Sentence	denotes	M, protein size standard; lane 1, whole cell extract before induction; lane 2, whole cell extract 15 h after induction. (c) 10% SDS-PAGE of purified Tα1-PAS.
T296	752-918	Sentence	denotes	M, protein size standard; lane 1, Tα1-PAS after ammonium sulfate precipitation, subtractive anion exchange (AEX), cation exchange (CEX), and final AEX chromatography.