PMC:7795856 / 34208-37646 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7795856","sourcedb":"PMC","sourceid":"7795856","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7795856","text":"Acknowledgments\nThe authors wish to thank Nicole Däschlein, Hyun-Jin Kim and Rebecca Walser, all at XL-protein, for help in gene cloning and western blotting and Corinna Brandt and Stefan Achatz at TUM for performing MS analyses. Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.\n\nAuthor Contributions\nConceptualization, U.B. and A.S.; experimental work, U.B.; manuscript preparation, U.B. and A.S. All authors have read and agreed to the published version of the manuscript.\n\nFunding\nThis research received no external funding.\n\nInstitutional Review Board Statement\nThe study was conducted in compliance with applicable animal welfare regulations (Institutional Animal Care and Use Committee approval No. 2020_ATRC/5999_F). The permission code for PK studies (PK, metabolism, and bioequivalence) was PE/EA/1374-5/2017 and the permitting authority was the Government Office for Pest County, Directorate of Food Chain Safety and Animal Health, Hungary.\n\nInformed Consent Statement\nNot applicable.\n\nData Availability Statement\nThe data presented in this study are available on request from the corresponding authors.\n\nConflicts of Interest\nU.B. and A.S. are co-founders and shareholders of XL-protein GmbH. Abbreviations\nAEX Anion exchange\nAP Alkaline phosphatase\naTc Anhydrotetracycline hydrochloride\nAUC Area und the curve\nBCIP 5-Bromo-4-chloro-3-indoxyl phosphate\nBSA Bovine serum albumin\nCEX Cation exchange\nCL Clearance\nCmax Maximum (or peak) serum concentration\nCNP C-type natriuretic peptide\nCORYNEX Corynebacterium glutamicum protein expression system\nCOVID-19 Severe acute respiratory syndrome coronavirus 2\nCTLA-4 Cytotoxic T-lymphocyte-associated protein 4\nCV Column volume\nDCs Dendritic cells\nDPP-IV Dipeptidyl peptidase-4\nELISA Enzyme-linked immunosorbent assay\nESETEC E. coli secretion technology\nESI-MS Electrospray ionization mass spectrometry\nFPLC Fast protein liquid chromatography\nGCC Glucagon\nGIP Glucose-dependent insulinotropic polypeptide\nGLP-1 Glucagon-like peptide 1\nGM-CSF Granulocyte-macrophage colony-stimulating factor\nGSH γ-L-Glutamyl-L-cysteinyl-glycine\nHBV Hepatitis B virus\nHCV Hepatitis C virus\nhGH Human growth hormone\nhIFN-α Human interferon 1\nHIV-1 Human immunodeficiency virus I\niRGD Internalizing arginylglycylaspartic acid peptide\nK3-EDTA Tri-potassium ethylenediaminetetraacetic acid\nMHC Major histocompatibility complex\nMOPS 3-(N-morpholino)propanesulfonic acid\nNBT Nitro blue tetrazolium\nOD Optical density at 550 nm\nPAS Proline/alanine-rich sequence\nPAS-Tα1 N-terminally PASylated Tα1\nPBS Phosphate-buffered saline\nPD Pharmacodynamics\nPEG Polyethylene glycol\np.i. Post injection\nPK Pharmacokinetics\nProTα Prothymosin alpha\nPTH Parathyroid hormone\nQ-TOF Quadrupole time of flight\nRBS Ribosome-binding site\nRGD Arginylglycylaspartic acid\nRimJ Ribosomal-protein-S5-alanine N-acetyltransferase\nRPC Reverse phase chromatography\nRT Room temperature\nSEC Size exclusion chromatography\nSDS-PAGE Sodium dodecyl sulfate polyacrylamide gel electrophoresis\nτ1/2α Half-life of distribution\nτ1/2β Elimination half-life\nTα1 Thymosin alpha 1\nTα1-PAS C-terminally PASylated Thymosin alpha 1\ntetp/o Tetracycline promoter/operator\nTh T helper cell\ntlpp Lipoprotein terminator\nTLR-2 Toll-like receptor 1\nTLR-9 Toll-like receptor 2\nTMAE Trimethylaminoethyl\ntmax Time to reach 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