PMC:7795856 / 30041-30770 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7795856","sourcedb":"PMC","sourceid":"7795856","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7795856","text":"Directly after the CEX chromatography, a 200 µl sample of PAS-Tα1 or PAS-Tα1 was adjusted to 2% v/v acetonitrile, 0.1% v/v formic acid and applied to a Resource RPC 1 mL column (GE Healthcare) equilibrated with 2% v/v acetonitrile, 0.1% v/v formic acid. The protein was eluted using a concentration gradient of up to 80% v/v acetonitrile, 0.1% v/v formic acid over 20 column volumes at a flow rate of 2 mL/min while spectrophotometrically monitoring protein elution at 225 nm. The eluted protein fraction was directly injected into a maXis quadrupole time of flight (Q-TOF) instrument (Bruker Daltonics, Bremen, Germany) operated in the positive ion mode. Raw data was deconvoluted using the MaxEntX algorithm (Bruker Daltonics).","tracks":[{"project":"LitCovid-PubTator","denotations":[{"id":"541","span":{"begin":62,"end":65},"obj":"Gene"},{"id":"542","span":{"begin":73,"end":76},"obj":"Gene"},{"id":"543","span":{"begin":161,"end":166},"obj":"Gene"},{"id":"544","span":{"begin":100,"end":112},"obj":"Chemical"},{"id":"545","span":{"begin":123,"end":134},"obj":"Chemical"},{"id":"546","span":{"begin":218,"end":230},"obj":"Chemical"},{"id":"547","span":{"begin":241,"end":252},"obj":"Chemical"},{"id":"548","span":{"begin":325,"end":337},"obj":"Chemical"},{"id":"549","span":{"begin":348,"end":359},"obj":"Chemical"}],"attributes":[{"id":"A541","pred":"tao:has_database_id","subj":"541","obj":"Gene:134864"},{"id":"A542","pred":"tao:has_database_id","subj":"542","obj":"Gene:134864"},{"id":"A543","pred":"tao:has_database_id","subj":"543","obj":"Gene:11128"},{"id":"A544","pred":"tao:has_database_id","subj":"544","obj":"MESH:C032159"},{"id":"A545","pred":"tao:has_database_id","subj":"545","obj":"MESH:C030544"},{"id":"A546","pred":"tao:has_database_id","subj":"546","obj":"MESH:C032159"},{"id":"A547","pred":"tao:has_database_id","subj":"547","obj":"MESH:C030544"},{"id":"A548","pred":"tao:has_database_id","subj":"548","obj":"MESH:C032159"},{"id":"A549","pred":"tao:has_database_id","subj":"549","obj":"MESH:C030544"},{"subj":"541","pred":"source","obj":"LitCovid-PubTator"},{"subj":"542","pred":"source","obj":"LitCovid-PubTator"},{"subj":"543","pred":"source","obj":"LitCovid-PubTator"},{"subj":"544","pred":"source","obj":"LitCovid-PubTator"},{"subj":"545","pred":"source","obj":"LitCovid-PubTator"},{"subj":"546","pred":"source","obj":"LitCovid-PubTator"},{"subj":"547","pred":"source","obj":"LitCovid-PubTator"},{"subj":"548","pred":"source","obj":"LitCovid-PubTator"},{"subj":"549","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T178","span":{"begin":0,"end":253},"obj":"Sentence"},{"id":"T179","span":{"begin":254,"end":476},"obj":"Sentence"},{"id":"T180","span":{"begin":477,"end":655},"obj":"Sentence"},{"id":"T181","span":{"begin":656,"end":729},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T178","pred":"source","obj":"LitCovid-sentences"},{"subj":"T179","pred":"source","obj":"LitCovid-sentences"},{"subj":"T180","pred":"source","obj":"LitCovid-sentences"},{"subj":"T181","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PubTator","color":"#b693ec","default":true},{"id":"LitCovid-sentences","color":"#93ec9c"}]}]}}