PMC:7795856 / 30041-30770 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue	tao:has_database_id
541	62-65	Gene	denotes	Tα1	Gene:134864
542	73-76	Gene	denotes	Tα1	Gene:134864
543	161-166	Gene	denotes	RPC 1	Gene:11128
544	100-112	Chemical	denotes	acetonitrile	MESH:C032159
545	123-134	Chemical	denotes	formic acid	MESH:C030544
546	218-230	Chemical	denotes	acetonitrile	MESH:C032159
547	241-252	Chemical	denotes	formic acid	MESH:C030544
548	325-337	Chemical	denotes	acetonitrile	MESH:C032159
549	348-359	Chemical	denotes	formic acid	MESH:C030544

Id	Subject	Object	Predicate	Lexical cue
T178	0-253	Sentence	denotes	Directly after the CEX chromatography, a 200 µl sample of PAS-Tα1 or PAS-Tα1 was adjusted to 2% v/v acetonitrile, 0.1% v/v formic acid and applied to a Resource RPC 1 mL column (GE Healthcare) equilibrated with 2% v/v acetonitrile, 0.1% v/v formic acid.
T179	254-476	Sentence	denotes	The protein was eluted using a concentration gradient of up to 80% v/v acetonitrile, 0.1% v/v formic acid over 20 column volumes at a flow rate of 2 mL/min while spectrophotometrically monitoring protein elution at 225 nm.
T180	477-655	Sentence	denotes	The eluted protein fraction was directly injected into a maXis quadrupole time of flight (Q-TOF) instrument (Bruker Daltonics, Bremen, Germany) operated in the positive ion mode.
T181	656-729	Sentence	denotes	Raw data was deconvoluted using the MaxEntX algorithm (Bruker Daltonics).