PMC:7795856 / 29975-30770 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"541","span":{"begin":128,"end":131},"obj":"Gene"},{"id":"542","span":{"begin":139,"end":142},"obj":"Gene"},{"id":"543","span":{"begin":227,"end":232},"obj":"Gene"},{"id":"544","span":{"begin":166,"end":178},"obj":"Chemical"},{"id":"545","span":{"begin":189,"end":200},"obj":"Chemical"},{"id":"546","span":{"begin":284,"end":296},"obj":"Chemical"},{"id":"547","span":{"begin":307,"end":318},"obj":"Chemical"},{"id":"548","span":{"begin":391,"end":403},"obj":"Chemical"},{"id":"549","span":{"begin":414,"end":425},"obj":"Chemical"}],"attributes":[{"id":"A541","pred":"tao:has_database_id","subj":"541","obj":"Gene:134864"},{"id":"A542","pred":"tao:has_database_id","subj":"542","obj":"Gene:134864"},{"id":"A543","pred":"tao:has_database_id","subj":"543","obj":"Gene:11128"},{"id":"A544","pred":"tao:has_database_id","subj":"544","obj":"MESH:C032159"},{"id":"A545","pred":"tao:has_database_id","subj":"545","obj":"MESH:C030544"},{"id":"A546","pred":"tao:has_database_id","subj":"546","obj":"MESH:C032159"},{"id":"A547","pred":"tao:has_database_id","subj":"547","obj":"MESH:C030544"},{"id":"A548","pred":"tao:has_database_id","subj":"548","obj":"MESH:C032159"},{"id":"A549","pred":"tao:has_database_id","subj":"549","obj":"MESH:C030544"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"4.7. Reverse-Phase Chromatography (RPC) and ESI Mass Spectrometry\nDirectly after the CEX chromatography, a 200 µl sample of PAS-Tα1 or PAS-Tα1 was adjusted to 2% v/v acetonitrile, 0.1% v/v formic acid and applied to a Resource RPC 1 mL column (GE Healthcare) equilibrated with 2% v/v acetonitrile, 0.1% v/v formic acid. The protein was eluted using a concentration gradient of up to 80% v/v acetonitrile, 0.1% v/v formic acid over 20 column volumes at a flow rate of 2 mL/min while spectrophotometrically monitoring protein elution at 225 nm. The eluted protein fraction was directly injected into a maXis quadrupole time of flight (Q-TOF) instrument (Bruker Daltonics, Bremen, Germany) operated in the positive ion mode. Raw data was deconvoluted using the MaxEntX algorithm (Bruker Daltonics)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T176","span":{"begin":0,"end":4},"obj":"Sentence"},{"id":"T177","span":{"begin":5,"end":65},"obj":"Sentence"},{"id":"T178","span":{"begin":66,"end":319},"obj":"Sentence"},{"id":"T179","span":{"begin":320,"end":542},"obj":"Sentence"},{"id":"T180","span":{"begin":543,"end":721},"obj":"Sentence"},{"id":"T181","span":{"begin":722,"end":795},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"4.7. Reverse-Phase Chromatography (RPC) and ESI Mass Spectrometry\nDirectly after the CEX chromatography, a 200 µl sample of PAS-Tα1 or PAS-Tα1 was adjusted to 2% v/v acetonitrile, 0.1% v/v formic acid and applied to a Resource RPC 1 mL column (GE Healthcare) equilibrated with 2% v/v acetonitrile, 0.1% v/v formic acid. The protein was eluted using a concentration gradient of up to 80% v/v acetonitrile, 0.1% v/v formic acid over 20 column volumes at a flow rate of 2 mL/min while spectrophotometrically monitoring protein elution at 225 nm. The eluted protein fraction was directly injected into a maXis quadrupole time of flight (Q-TOF) instrument (Bruker Daltonics, Bremen, Germany) operated in the positive ion mode. Raw data was deconvoluted using the MaxEntX algorithm (Bruker Daltonics)."}