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PMC:7795856 / 29975-30770 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
541 128-131 Gene denotes Tα1 Gene:134864
542 139-142 Gene denotes Tα1 Gene:134864
543 227-232 Gene denotes RPC 1 Gene:11128
544 166-178 Chemical denotes acetonitrile MESH:C032159
545 189-200 Chemical denotes formic acid MESH:C030544
546 284-296 Chemical denotes acetonitrile MESH:C032159
547 307-318 Chemical denotes formic acid MESH:C030544
548 391-403 Chemical denotes acetonitrile MESH:C032159
549 414-425 Chemical denotes formic acid MESH:C030544

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T176 0-4 Sentence denotes 4.7.
T177 5-65 Sentence denotes Reverse-Phase Chromatography (RPC) and ESI Mass Spectrometry
T178 66-319 Sentence denotes Directly after the CEX chromatography, a 200 µl sample of PAS-Tα1 or PAS-Tα1 was adjusted to 2% v/v acetonitrile, 0.1% v/v formic acid and applied to a Resource RPC 1 mL column (GE Healthcare) equilibrated with 2% v/v acetonitrile, 0.1% v/v formic acid.
T179 320-542 Sentence denotes The protein was eluted using a concentration gradient of up to 80% v/v acetonitrile, 0.1% v/v formic acid over 20 column volumes at a flow rate of 2 mL/min while spectrophotometrically monitoring protein elution at 225 nm.
T180 543-721 Sentence denotes The eluted protein fraction was directly injected into a maXis quadrupole time of flight (Q-TOF) instrument (Bruker Daltonics, Bremen, Germany) operated in the positive ion mode.
T181 722-795 Sentence denotes Raw data was deconvoluted using the MaxEntX algorithm (Bruker Daltonics).