PMC:7795856 / 283-780 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7795856","sourcedb":"PMC","sourceid":"7795856","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7795856","text":"ich also offers prospects in the context of COVID-19 infections and cancer. Manufacturing of this N-terminally acetylated 28-residue peptide is demanding, and its short plasma half-life limits in vivo efficacy and requires frequent dosing. Here, we combined the PASylation technology with enzymatic in situ N-acetylation by RimJ to produce a long-acting version of Tα1 in Escherichia coli at high yield. ESI-MS analysis of the purified fusion protein indicated the expected composition without any","tracks":[{"project":"LitCovid-PubTator","denotations":[{"id":"19","span":{"begin":365,"end":368},"obj":"Gene"},{"id":"21","span":{"begin":372,"end":388},"obj":"Species"},{"id":"25","span":{"begin":307,"end":308},"obj":"Chemical"},{"id":"26","span":{"begin":324,"end":328},"obj":"Chemical"},{"id":"32","span":{"begin":44,"end":52},"obj":"Disease"},{"id":"33","span":{"begin":53,"end":63},"obj":"Disease"},{"id":"34","span":{"begin":68,"end":74},"obj":"Disease"}],"attributes":[{"id":"A19","pred":"tao:has_database_id","subj":"19","obj":"Gene:134864"},{"id":"A21","pred":"tao:has_database_id","subj":"21","obj":"Tax:562"},{"id":"A25","pred":"tao:has_database_id","subj":"25","obj":"MESH:D009584"},{"id":"A32","pred":"tao:has_database_id","subj":"32","obj":"MESH:C000657245"},{"id":"A33","pred":"tao:has_database_id","subj":"33","obj":"MESH:D007239"},{"id":"A34","pred":"tao:has_database_id","subj":"34","obj":"MESH:D009369"},{"subj":"19","pred":"source","obj":"LitCovid-PubTator"},{"subj":"21","pred":"source","obj":"LitCovid-PubTator"},{"subj":"25","pred":"source","obj":"LitCovid-PubTator"},{"subj":"26","pred":"source","obj":"LitCovid-PubTator"},{"subj":"32","pred":"source","obj":"LitCovid-PubTator"},{"subj":"33","pred":"source","obj":"LitCovid-PubTator"},{"subj":"34","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-PD-HP","denotations":[{"id":"T4","span":{"begin":68,"end":74},"obj":"Phenotype"}],"attributes":[{"id":"A4","pred":"hp_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/HP_0002664"},{"subj":"T4","pred":"source","obj":"LitCovid-PD-HP"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T5","span":{"begin":76,"end":239},"obj":"Sentence"},{"id":"T6","span":{"begin":240,"end":403},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T5","pred":"source","obj":"LitCovid-sentences"},{"subj":"T6","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PubTator","color":"#93ecc2","default":true},{"id":"LitCovid-PD-HP","color":"#eca893"},{"id":"LitCovid-sentences","color":"#9397ec"}]}]}}