PMC:7795856 / 28014-28702 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"501","span":{"begin":289,"end":292},"obj":"Gene"},{"id":"502","span":{"begin":300,"end":303},"obj":"Gene"},{"id":"503","span":{"begin":526,"end":537},"obj":"Gene"},{"id":"504","span":{"begin":584,"end":595},"obj":"Gene"},{"id":"505","span":{"begin":500,"end":513},"obj":"Gene"},{"id":"506","span":{"begin":475,"end":488},"obj":"Gene"},{"id":"507","span":{"begin":86,"end":94},"obj":"Chemical"}],"attributes":[{"id":"A501","pred":"tao:has_database_id","subj":"501","obj":"Gene:134864"},{"id":"A502","pred":"tao:has_database_id","subj":"502","obj":"Gene:134864"},{"id":"A503","pred":"tao:has_database_id","subj":"503","obj":"Gene:2495"},{"id":"A504","pred":"tao:has_database_id","subj":"504","obj":"Gene:7018"},{"id":"A505","pred":"tao:has_database_id","subj":"505","obj":"Gene:7038"},{"id":"A506","pred":"tao:has_database_id","subj":"506","obj":"Gene:7038"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"4.4. Analytical Size Exclusion Chromatography\nAnalytical SEC was performed on a 24 mL Superose® 6 10/300 GL column (GE Healthcare) using an ÄKTA Explorer 10 system operated at a flow rate of 0.5 mL/min with PBS as the running buffer. To determine the apparent molecular size, purified PAS-Tα1 or PAS-Tα1 (100 µL) was applied to the column and the elution volume was used for linear interpolation from a half-logarithmic calibration line obtained using the reference proteins thyroglobulin (octamer), thyroglobulin (tetramer), apoferritin, β-amylase, alcohol dehydrogenase (tetramer), transferrin, ovalbumin, and carboanhydrase as well as blue dextran (all from Merck, Darmstadt, Germany)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T163","span":{"begin":0,"end":4},"obj":"Sentence"},{"id":"T164","span":{"begin":5,"end":45},"obj":"Sentence"},{"id":"T165","span":{"begin":46,"end":233},"obj":"Sentence"},{"id":"T166","span":{"begin":234,"end":688},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"4.4. Analytical Size Exclusion Chromatography\nAnalytical SEC was performed on a 24 mL Superose® 6 10/300 GL column (GE Healthcare) using an ÄKTA Explorer 10 system operated at a flow rate of 0.5 mL/min with PBS as the running buffer. To determine the apparent molecular size, purified PAS-Tα1 or PAS-Tα1 (100 µL) was applied to the column and the elution volume was used for linear interpolation from a half-logarithmic calibration line obtained using the reference proteins thyroglobulin (octamer), thyroglobulin (tetramer), apoferritin, β-amylase, alcohol dehydrogenase (tetramer), transferrin, ovalbumin, and carboanhydrase as well as blue dextran (all from Merck, Darmstadt, Germany)."}