PMC:7795856 / 28014-28702 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue	tao:has_database_id
501	289-292	Gene	denotes	Tα1	Gene:134864
502	300-303	Gene	denotes	Tα1	Gene:134864
503	526-537	Gene	denotes	apoferritin	Gene:2495
504	584-595	Gene	denotes	transferrin	Gene:7018
505	500-513	Gene	denotes	thyroglobulin	Gene:7038
506	475-488	Gene	denotes	thyroglobulin	Gene:7038
507	86-94	Chemical	denotes	Superose

Id	Subject	Object	Predicate	Lexical cue
T163	0-4	Sentence	denotes	4.4.
T164	5-45	Sentence	denotes	Analytical Size Exclusion Chromatography
T165	46-233	Sentence	denotes	Analytical SEC was performed on a 24 mL Superose® 6 10/300 GL column (GE Healthcare) using an ÄKTA Explorer 10 system operated at a flow rate of 0.5 mL/min with PBS as the running buffer.
T166	234-688	Sentence	denotes	To determine the apparent molecular size, purified PAS-Tα1 or PAS-Tα1 (100 µL) was applied to the column and the elution volume was used for linear interpolation from a half-logarithmic calibration line obtained using the reference proteins thyroglobulin (octamer), thyroglobulin (tetramer), apoferritin, β-amylase, alcohol dehydrogenase (tetramer), transferrin, ovalbumin, and carboanhydrase as well as blue dextran (all from Merck, Darmstadt, Germany).