PMC:7795856 / 24966-25786 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7795856","sourcedb":"PMC","sourceid":"7795856","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7795856","text":"4.2. Bacterial Production of Recombinant PASylated Tα1\nThe BL21 derivative E. coli strain NEBexpress® (New England Biolabs, Frankfurt am Main, Germany) was transformed with the plasmids pASK75-Tα1-PAS#1(600)/RimJ or pASK75-MP-PAS#1(600)-Tα1 from above. Two milliliters of Terrific Broth supplemented with 100 µg/mL ampicillin (TBamp) was inoculated with a single colony and grown overnight at 37 °C, 170 rpm. This overnight culture was used to inoculate a 50 mL preculture, which was grown under the same conditions until OD550 = 1.1 was reached. Subsequently, the 50 mL preculture was transferred into 2 L TBamp and incubated in a 5 L baffled flask at 37 °C and 100 rpm. After reaching OD550 = 0.6, the temperature was decreased to 26 °C and the cells were subsequently induced at OD550 = 1 with 200 µg/mL aTc for 15 h.","divisions":[{"label":"title","span":{"begin":0,"end":54}}],"tracks":[{"project":"LitCovid-PubTator","denotations":[{"id":"431","span":{"begin":237,"end":240},"obj":"Gene"},{"id":"432","span":{"begin":75,"end":82},"obj":"Species"},{"id":"433","span":{"begin":315,"end":325},"obj":"Chemical"},{"id":"434","span":{"begin":327,"end":332},"obj":"Chemical"},{"id":"435","span":{"begin":607,"end":612},"obj":"Chemical"}],"attributes":[{"id":"A431","pred":"tao:has_database_id","subj":"431","obj":"Gene:134864"},{"id":"A432","pred":"tao:has_database_id","subj":"432","obj":"Tax:562"},{"id":"A433","pred":"tao:has_database_id","subj":"433","obj":"MESH:D000667"},{"id":"A434","pred":"tao:has_database_id","subj":"434","obj":"MESH:C064881"},{"id":"A435","pred":"tao:has_database_id","subj":"435","obj":"MESH:C064881"},{"subj":"431","pred":"source","obj":"LitCovid-PubTator"},{"subj":"432","pred":"source","obj":"LitCovid-PubTator"},{"subj":"433","pred":"source","obj":"LitCovid-PubTator"},{"subj":"434","pred":"source","obj":"LitCovid-PubTator"},{"subj":"435","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T141","span":{"begin":0,"end":4},"obj":"Sentence"},{"id":"T142","span":{"begin":5,"end":54},"obj":"Sentence"},{"id":"T143","span":{"begin":55,"end":252},"obj":"Sentence"},{"id":"T144","span":{"begin":253,"end":408},"obj":"Sentence"},{"id":"T145","span":{"begin":409,"end":546},"obj":"Sentence"},{"id":"T146","span":{"begin":547,"end":671},"obj":"Sentence"},{"id":"T147","span":{"begin":672,"end":820},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T141","pred":"source","obj":"LitCovid-sentences"},{"subj":"T142","pred":"source","obj":"LitCovid-sentences"},{"subj":"T143","pred":"source","obj":"LitCovid-sentences"},{"subj":"T144","pred":"source","obj":"LitCovid-sentences"},{"subj":"T145","pred":"source","obj":"LitCovid-sentences"},{"subj":"T146","pred":"source","obj":"LitCovid-sentences"},{"subj":"T147","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PubTator","color":"#ec93a0","default":true},{"id":"LitCovid-sentences","color":"#93baec"}]}]}}