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PMC:7795856 / 24966-25786 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
431 237-240 Gene denotes Tα1 Gene:134864
432 75-82 Species denotes E. coli Tax:562
433 315-325 Chemical denotes ampicillin MESH:D000667
434 327-332 Chemical denotes TBamp MESH:C064881
435 607-612 Chemical denotes TBamp MESH:C064881

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T141 0-4 Sentence denotes 4.2.
T142 5-54 Sentence denotes Bacterial Production of Recombinant PASylated Tα1
T143 55-252 Sentence denotes The BL21 derivative E. coli strain NEBexpress® (New England Biolabs, Frankfurt am Main, Germany) was transformed with the plasmids pASK75-Tα1-PAS#1(600)/RimJ or pASK75-MP-PAS#1(600)-Tα1 from above.
T144 253-408 Sentence denotes Two milliliters of Terrific Broth supplemented with 100 µg/mL ampicillin (TBamp) was inoculated with a single colony and grown overnight at 37 °C, 170 rpm.
T145 409-546 Sentence denotes This overnight culture was used to inoculate a 50 mL preculture, which was grown under the same conditions until OD550 = 1.1 was reached.
T146 547-671 Sentence denotes Subsequently, the 50 mL preculture was transferred into 2 L TBamp and incubated in a 5 L baffled flask at 37 °C and 100 rpm.
T147 672-820 Sentence denotes After reaching OD550 = 0.6, the temperature was decreased to 26 °C and the cells were subsequently induced at OD550 = 1 with 200 µg/mL aTc for 15 h.