PMC:7795856 / 24966-25786 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue	tao:has_database_id
431	237-240	Gene	denotes	Tα1	Gene:134864
432	75-82	Species	denotes	E. coli	Tax:562
433	315-325	Chemical	denotes	ampicillin	MESH:D000667
434	327-332	Chemical	denotes	TBamp	MESH:C064881
435	607-612	Chemical	denotes	TBamp	MESH:C064881

Id	Subject	Object	Predicate	Lexical cue
T141	0-4	Sentence	denotes	4.2.
T142	5-54	Sentence	denotes	Bacterial Production of Recombinant PASylated Tα1
T143	55-252	Sentence	denotes	The BL21 derivative E. coli strain NEBexpress® (New England Biolabs, Frankfurt am Main, Germany) was transformed with the plasmids pASK75-Tα1-PAS#1(600)/RimJ or pASK75-MP-PAS#1(600)-Tα1 from above.
T144	253-408	Sentence	denotes	Two milliliters of Terrific Broth supplemented with 100 µg/mL ampicillin (TBamp) was inoculated with a single colony and grown overnight at 37 °C, 170 rpm.
T145	409-546	Sentence	denotes	This overnight culture was used to inoculate a 50 mL preculture, which was grown under the same conditions until OD550 = 1.1 was reached.
T146	547-671	Sentence	denotes	Subsequently, the 50 mL preculture was transferred into 2 L TBamp and incubated in a 5 L baffled flask at 37 °C and 100 rpm.
T147	672-820	Sentence	denotes	After reaching OD550 = 0.6, the temperature was decreased to 26 °C and the cells were subsequently induced at OD550 = 1 with 200 µg/mL aTc for 15 h.