PMC:7712180 / 28579-29550 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"981","span":{"begin":131,"end":152},"obj":"Gene"},{"id":"982","span":{"begin":183,"end":188},"obj":"Gene"},{"id":"983","span":{"begin":254,"end":267},"obj":"Gene"},{"id":"984","span":{"begin":651,"end":660},"obj":"Gene"},{"id":"985","span":{"begin":127,"end":130},"obj":"Species"},{"id":"986","span":{"begin":163,"end":169},"obj":"Species"},{"id":"987","span":{"begin":273,"end":287},"obj":"Species"},{"id":"988","span":{"begin":210,"end":213},"obj":"Species"},{"id":"989","span":{"begin":329,"end":332},"obj":"Species"},{"id":"990","span":{"begin":503,"end":506},"obj":"Species"},{"id":"991","span":{"begin":778,"end":781},"obj":"Species"},{"id":"992","span":{"begin":872,"end":875},"obj":"Species"},{"id":"993","span":{"begin":947,"end":950},"obj":"Species"},{"id":"994","span":{"begin":688,"end":694},"obj":"Chemical"},{"id":"995","span":{"begin":855,"end":858},"obj":"Chemical"}],"attributes":[{"id":"A981","pred":"tao:has_database_id","subj":"981","obj":"Gene:252922"},{"id":"A982","pred":"tao:has_database_id","subj":"982","obj":"Gene:281333"},{"id":"A983","pred":"tao:has_database_id","subj":"983","obj":"Gene:4758"},{"id":"A985","pred":"tao:has_database_id","subj":"985","obj":"Tax:10116"},{"id":"A986","pred":"tao:has_database_id","subj":"986","obj":"Tax:9913"},{"id":"A987","pred":"tao:has_database_id","subj":"987","obj":"Tax:37931"},{"id":"A988","pred":"tao:has_database_id","subj":"988","obj":"Tax:11552"},{"id":"A989","pred":"tao:has_database_id","subj":"989","obj":"Tax:11552"},{"id":"A990","pred":"tao:has_database_id","subj":"990","obj":"Tax:11552"},{"id":"A991","pred":"tao:has_database_id","subj":"991","obj":"Tax:11552"},{"id":"A992","pred":"tao:has_database_id","subj":"992","obj":"Tax:11552"},{"id":"A993","pred":"tao:has_database_id","subj":"993","obj":"Tax:11552"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Thin-layer chromatography (TLC), gas-liquid chromatography (GLC) and high-performance liquid chromatography (HPLC) analyses of rat alpha 1-macroglobulin (RMG) and bovine submaxillary mucin (BSM) incubated with ICV in comparison with those incubated with neuraminidase from A. ureafaciens revealed that RMG and BSM incubated with ICV have a reduced amount of Neu5,9Ac2 but an increased amount of Neu5Ac. After confirmation by using purified Neu5,9Ac2 instead of RMG and BSM, it was concluded that RDE of ICV is neuraminate O-acetylesterase (9-O-acetyl N-acetylneuraminate O-acetylhydrolase (EC 3.1.1.53) catalyzing removal of the 9-O-acetyl group from Neu5,9Ac2, not cleaving the terminal Neu5Ac from glycoconjugate [182]. RMG and BSM can potentially inhibit hemagglutination by ICV at 4oC, and their inhibitory effects were abolished by pre-incubation of RMG and BSM with ICV at 37oC [182]. This evidence suggested that Neu5,9Ac2 is a receptor of ICV on the cell surface."}

{"project":"LitCovid-sentences","denotations":[{"id":"T170","span":{"begin":0,"end":402},"obj":"Sentence"},{"id":"T171","span":{"begin":403,"end":721},"obj":"Sentence"},{"id":"T172","span":{"begin":722,"end":890},"obj":"Sentence"},{"id":"T173","span":{"begin":891,"end":971},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Thin-layer chromatography (TLC), gas-liquid chromatography (GLC) and high-performance liquid chromatography (HPLC) analyses of rat alpha 1-macroglobulin (RMG) and bovine submaxillary mucin (BSM) incubated with ICV in comparison with those incubated with neuraminidase from A. ureafaciens revealed that RMG and BSM incubated with ICV have a reduced amount of Neu5,9Ac2 but an increased amount of Neu5Ac. After confirmation by using purified Neu5,9Ac2 instead of RMG and BSM, it was concluded that RDE of ICV is neuraminate O-acetylesterase (9-O-acetyl N-acetylneuraminate O-acetylhydrolase (EC 3.1.1.53) catalyzing removal of the 9-O-acetyl group from Neu5,9Ac2, not cleaving the terminal Neu5Ac from glycoconjugate [182]. RMG and BSM can potentially inhibit hemagglutination by ICV at 4oC, and their inhibitory effects were abolished by pre-incubation of RMG and BSM with ICV at 37oC [182]. This evidence suggested that Neu5,9Ac2 is a receptor of ICV on the cell surface."}