PMC:7668588 / 10157-10725 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"163","span":{"begin":258,"end":261},"obj":"Gene"},{"id":"164","span":{"begin":500,"end":504},"obj":"Gene"},{"id":"165","span":{"begin":405,"end":415},"obj":"Species"}],"attributes":[{"id":"A163","pred":"tao:has_database_id","subj":"163","obj":"Gene:3815"},{"id":"A164","pred":"tao:has_database_id","subj":"164","obj":"Gene:27074"},{"id":"A165","pred":"tao:has_database_id","subj":"165","obj":"Tax:2697049"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Validation of the assays using clinical specimens\nA validation study was performed using 224 nasal swab samples collected in Nagasaki prefecture, Japan, without any clinical information. For RT-LAMP and RT-qPCR, RNA was extracted using QIAamp Viral RNA Mini Kit (QIAGEN) according to the instructions of the manufacturer. For Direct RT-LAMP, the samples were heated at 95°C for 10 min for inactivation of SARS-CoV-2. Aliquots of 5 μL of RNA samples or heat inactivated swab samples were used for the LAMP assay with ORF1b-1 primer set and RT-qPCR with NIID primer set."}

{"project":"LitCovid-sentences","denotations":[{"id":"T83","span":{"begin":0,"end":49},"obj":"Sentence"},{"id":"T84","span":{"begin":50,"end":186},"obj":"Sentence"},{"id":"T85","span":{"begin":187,"end":321},"obj":"Sentence"},{"id":"T86","span":{"begin":322,"end":416},"obj":"Sentence"},{"id":"T87","span":{"begin":417,"end":568},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Validation of the assays using clinical specimens\nA validation study was performed using 224 nasal swab samples collected in Nagasaki prefecture, Japan, without any clinical information. For RT-LAMP and RT-qPCR, RNA was extracted using QIAamp Viral RNA Mini Kit (QIAGEN) according to the instructions of the manufacturer. For Direct RT-LAMP, the samples were heated at 95°C for 10 min for inactivation of SARS-CoV-2. Aliquots of 5 μL of RNA samples or heat inactivated swab samples were used for the LAMP assay with ORF1b-1 primer set and RT-qPCR with NIID primer set."}