| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T83 |
0-49 |
Sentence |
denotes |
Validation of the assays using clinical specimens |
| T84 |
50-186 |
Sentence |
denotes |
A validation study was performed using 224 nasal swab samples collected in Nagasaki prefecture, Japan, without any clinical information. |
| T85 |
187-321 |
Sentence |
denotes |
For RT-LAMP and RT-qPCR, RNA was extracted using QIAamp Viral RNA Mini Kit (QIAGEN) according to the instructions of the manufacturer. |
| T86 |
322-416 |
Sentence |
denotes |
For Direct RT-LAMP, the samples were heated at 95°C for 10 min for inactivation of SARS-CoV-2. |
| T87 |
417-568 |
Sentence |
denotes |
Aliquots of 5 μL of RNA samples or heat inactivated swab samples were used for the LAMP assay with ORF1b-1 primer set and RT-qPCR with NIID primer set. |
