PMC:7650537 / 8114-8794 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T58","span":{"begin":133,"end":138},"obj":"Body_part"},{"id":"T59","span":{"begin":232,"end":237},"obj":"Body_part"},{"id":"T60","span":{"begin":337,"end":341},"obj":"Body_part"},{"id":"T61","span":{"begin":391,"end":395},"obj":"Body_part"},{"id":"T62","span":{"begin":396,"end":401},"obj":"Body_part"},{"id":"T63","span":{"begin":501,"end":506},"obj":"Body_part"}],"attributes":[{"id":"A58","pred":"fma_id","subj":"T58","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A59","pred":"fma_id","subj":"T59","obj":"http://purl.org/sig/ont/fma/fma63083"},{"id":"A60","pred":"fma_id","subj":"T60","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A61","pred":"fma_id","subj":"T61","obj":"http://purl.org/sig/ont/fma/fma24984"},{"id":"A62","pred":"fma_id","subj":"T62","obj":"http://purl.org/sig/ont/fma/fma63083"},{"id":"A63","pred":"fma_id","subj":"T63","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":" each dilution step with 50 µL virus suspension containing 100 plaque-forming units, followed by an incubation for 1 h at 37 °C. The cells were washed with phosphate-buffered saline (Bio\u0026SELL), inoculated with 100 μL of these virus serum dilutions, and incubated for one hour at room temperature on a rocking shaker. Then, 100 µL of the cell culture medium supplemented with 20% (v/v) fetal calf serum (FCS) was added to each well to achieve a FCS concentration of 10% (v/v). After four to five days, cells were fixed with 4% (w/v) paraformaldehyde in phosphate-buffered saline and stained with an aqueous solution of 0.1% (w/v) crystal violet and 20% (v/v) methanol. The stained "}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T6","span":{"begin":232,"end":237},"obj":"Body_part"},{"id":"T7","span":{"begin":396,"end":401},"obj":"Body_part"}],"attributes":[{"id":"A6","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/UBERON_0001977"},{"id":"A7","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":" each dilution step with 50 µL virus suspension containing 100 plaque-forming units, followed by an incubation for 1 h at 37 °C. The cells were washed with phosphate-buffered saline (Bio\u0026SELL), inoculated with 100 μL of these virus serum dilutions, and incubated for one hour at room temperature on a rocking shaker. Then, 100 µL of the cell culture medium supplemented with 20% (v/v) fetal calf serum (FCS) was added to each well to achieve a FCS concentration of 10% (v/v). After four to five days, cells were fixed with 4% (w/v) paraformaldehyde in phosphate-buffered saline and stained with an aqueous solution of 0.1% (w/v) crystal violet and 20% (v/v) methanol. The stained "}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T67","span":{"begin":31,"end":36},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T68","span":{"begin":133,"end":138},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T69","span":{"begin":187,"end":191},"obj":"http://purl.obolibrary.org/obo/PR_000001318"},{"id":"T70","span":{"begin":226,"end":231},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T71","span":{"begin":299,"end":300},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T72","span":{"begin":337,"end":341},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T73","span":{"begin":391,"end":395},"obj":"http://www.ebi.ac.uk/efo/EFO_0003051"},{"id":"T74","span":{"begin":442,"end":443},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T75","span":{"begin":501,"end":506},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T76","span":{"begin":527,"end":530},"obj":"http://purl.obolibrary.org/obo/CLO_0050644"},{"id":"T77","span":{"begin":624,"end":627},"obj":"http://purl.obolibrary.org/obo/CLO_0050644"}],"text":" each dilution step with 50 µL virus suspension containing 100 plaque-forming units, followed by an incubation for 1 h at 37 °C. The cells were washed with phosphate-buffered saline (Bio\u0026SELL), inoculated with 100 μL of these virus serum dilutions, and incubated for one hour at room temperature on a rocking shaker. Then, 100 µL of the cell culture medium supplemented with 20% (v/v) fetal calf serum (FCS) was added to each well to achieve a FCS concentration of 10% (v/v). After four to five days, cells were fixed with 4% (w/v) paraformaldehyde in phosphate-buffered saline and stained with an aqueous solution of 0.1% (w/v) crystal violet and 20% (v/v) methanol. The stained "}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T33","span":{"begin":156,"end":165},"obj":"Chemical"},{"id":"T37","span":{"begin":552,"end":561},"obj":"Chemical"},{"id":"T41","span":{"begin":606,"end":614},"obj":"Chemical"},{"id":"T42","span":{"begin":629,"end":643},"obj":"Chemical"},{"id":"T43","span":{"begin":658,"end":666},"obj":"Chemical"}],"attributes":[{"id":"A33","pred":"chebi_id","subj":"T33","obj":"http://purl.obolibrary.org/obo/CHEBI_18367"},{"id":"A34","pred":"chebi_id","subj":"T33","obj":"http://purl.obolibrary.org/obo/CHEBI_26020"},{"id":"A35","pred":"chebi_id","subj":"T33","obj":"http://purl.obolibrary.org/obo/CHEBI_35780"},{"id":"A36","pred":"chebi_id","subj":"T33","obj":"http://purl.obolibrary.org/obo/CHEBI_43474"},{"id":"A37","pred":"chebi_id","subj":"T37","obj":"http://purl.obolibrary.org/obo/CHEBI_18367"},{"id":"A38","pred":"chebi_id","subj":"T37","obj":"http://purl.obolibrary.org/obo/CHEBI_26020"},{"id":"A39","pred":"chebi_id","subj":"T37","obj":"http://purl.obolibrary.org/obo/CHEBI_35780"},{"id":"A40","pred":"chebi_id","subj":"T37","obj":"http://purl.obolibrary.org/obo/CHEBI_43474"},{"id":"A41","pred":"chebi_id","subj":"T41","obj":"http://purl.obolibrary.org/obo/CHEBI_75958"},{"id":"A42","pred":"chebi_id","subj":"T42","obj":"http://purl.obolibrary.org/obo/CHEBI_41688"},{"id":"A43","pred":"chebi_id","subj":"T43","obj":"http://purl.obolibrary.org/obo/CHEBI_17790"}],"text":" each dilution step with 50 µL virus suspension containing 100 plaque-forming units, followed by an incubation for 1 h at 37 °C. The cells were washed with phosphate-buffered saline (Bio\u0026SELL), inoculated with 100 μL of these virus serum dilutions, and incubated for one hour at room temperature on a rocking shaker. Then, 100 µL of the cell culture medium supplemented with 20% (v/v) fetal calf serum (FCS) was added to each well to achieve a FCS concentration of 10% (v/v). After four to five days, cells were fixed with 4% (w/v) paraformaldehyde in phosphate-buffered saline and stained with an aqueous solution of 0.1% (w/v) crystal violet and 20% (v/v) methanol. The stained "}

{"project":"LitCovid-sentences","denotations":[{"id":"T63","span":{"begin":129,"end":316},"obj":"Sentence"},{"id":"T64","span":{"begin":317,"end":475},"obj":"Sentence"},{"id":"T65","span":{"begin":476,"end":667},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":" each dilution step with 50 µL virus suspension containing 100 plaque-forming units, followed by an incubation for 1 h at 37 °C. The cells were washed with phosphate-buffered saline (Bio\u0026SELL), inoculated with 100 μL of these virus serum dilutions, and incubated for one hour at room temperature on a rocking shaker. Then, 100 µL of the cell culture medium supplemented with 20% (v/v) fetal calf serum (FCS) was added to each well to achieve a FCS concentration of 10% (v/v). After four to five days, cells were fixed with 4% (w/v) paraformaldehyde in phosphate-buffered saline and stained with an aqueous solution of 0.1% (w/v) crystal violet and 20% (v/v) methanol. The stained "}

{"project":"LitCovid-PubTator","denotations":[{"id":"162","span":{"begin":156,"end":181},"obj":"Chemical"},{"id":"163","span":{"begin":532,"end":548},"obj":"Chemical"},{"id":"164","span":{"begin":552,"end":577},"obj":"Chemical"},{"id":"165","span":{"begin":629,"end":643},"obj":"Chemical"},{"id":"166","span":{"begin":658,"end":666},"obj":"Chemical"}],"attributes":[{"id":"A163","pred":"tao:has_database_id","subj":"163","obj":"MESH:C003043"},{"id":"A165","pred":"tao:has_database_id","subj":"165","obj":"MESH:D005840"},{"id":"A166","pred":"tao:has_database_id","subj":"166","obj":"MESH:D000432"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":" each dilution step with 50 µL virus suspension containing 100 plaque-forming units, followed by an incubation for 1 h at 37 °C. The cells were washed with phosphate-buffered saline (Bio\u0026SELL), inoculated with 100 μL of these virus serum dilutions, and incubated for one hour at room temperature on a rocking shaker. Then, 100 µL of the cell culture medium supplemented with 20% (v/v) fetal calf serum (FCS) was added to each well to achieve a FCS concentration of 10% (v/v). After four to five days, cells were fixed with 4% (w/v) paraformaldehyde in phosphate-buffered saline and stained with an aqueous solution of 0.1% (w/v) crystal violet and 20% (v/v) methanol. The stained "}