PMC:7640975 / 8591-9197 JSONTXT

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{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7640975","sourcedb":"PMC","sourceid":"7640975","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7640975","text":"The kinetics of PDCoV multiplication in IPEC-J2 cells was determined by measuring the TCID50 using a microtitration infectivity assay. Briefly, 100 μL/well of 10-fold serial dilutions of PDCoV were inoculated onto IPEC-J2 cells grown in 96-well microplates (Corning, NY, USA). After adsorption at 37 °C for 1.5 h, the inocula were removed, and serum-free DMEM/F12 medium containing 5 μg/mL of trypsin was added to the wells. 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