PMC:7640975 / 745-2099 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7640975","sourcedb":"PMC","sourceid":"7640975","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7640975","text":"are available via ProteomeXchange with identifier PXD019975. To ensure reliability of the proteomics data, two randomly selected DEPs, the downregulated anaphase-promoting complex subunit 7 (ANAPC7) and upregulated interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), were verified by real-time PCR and Western blot, and the results of which indicate that the proteomics data were reliable and valid. Bioinformatics analyses, including GO, COG, KEGG, and STRING, further demonstrated that a majority of the DEPs are involved in numerous crucial biological processes and signaling pathways, such as immune system, digestive system, signal transduction, RIG-I-like receptor, mTOR, PI3K-AKT, autophagy, and cell cycle signaling pathways. Altogether, this is the first study on proteomes of PDCoV-infected host cells, which shall provide valuable clues for further investigation of PDCoV pathogenesis.\n\n1 Introduction\nPorcine deltacoronavirus (PDCoV) is enveloped and has a single-stranded, positive-sense RNA genome, which is classified in the genus Deltacoronavirus within the Coronaviridae family.1 Although PDCoV was initially identified in rectal swabs of pigs during a molecular epidemiological investigation conducted in Hong Kong, China in 2012,2 diarrheal diseases in pigs associated with PDCoV infection were first recorded in the 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