PMC:7640975 / 36364-37419 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T312","span":{"begin":32,"end":36},"obj":"Body_part"},{"id":"T313","span":{"begin":68,"end":71},"obj":"Body_part"},{"id":"T314","span":{"begin":229,"end":234},"obj":"Body_part"},{"id":"T315","span":{"begin":255,"end":259},"obj":"Body_part"},{"id":"T316","span":{"begin":429,"end":434},"obj":"Body_part"},{"id":"T317","span":{"begin":468,"end":473},"obj":"Body_part"},{"id":"T318","span":{"begin":677,"end":685},"obj":"Body_part"},{"id":"T319","span":{"begin":697,"end":702},"obj":"Body_part"},{"id":"T320","span":{"begin":733,"end":738},"obj":"Body_part"},{"id":"T321","span":{"begin":828,"end":832},"obj":"Body_part"},{"id":"T322","span":{"begin":849,"end":854},"obj":"Body_part"},{"id":"T323","span":{"begin":1003,"end":1008},"obj":"Body_part"}],"attributes":[{"id":"A312","pred":"fma_id","subj":"T312","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A313","pred":"fma_id","subj":"T313","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A314","pred":"fma_id","subj":"T314","obj":"http://purl.org/sig/ont/fma/fma67843"},{"id":"A315","pred":"fma_id","subj":"T315","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A316","pred":"fma_id","subj":"T316","obj":"http://purl.org/sig/ont/fma/fma67843"},{"id":"A317","pred":"fma_id","subj":"T317","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A318","pred":"fma_id","subj":"T318","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A319","pred":"fma_id","subj":"T319","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A320","pred":"fma_id","subj":"T320","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A321","pred":"fma_id","subj":"T321","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A322","pred":"fma_id","subj":"T322","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A323","pred":"fma_id","subj":"T323","obj":"http://purl.org/sig/ont/fma/fma67843"}],"text":" strain at an MOI of 0.1 TCID50/cell and collected at 24 hpi. Total RNA was extracted and reverse-transcribed into cDNA for the subsequent analysis via qPCR. Fold-change values were calculated based on the 2–ΔΔCt method, using β-actin as the housekeeping gene. Error bars indicate the standard error of three independent experiments (Student’s t test; *p \u003c 0.05). (B) The relative ratio of ANAPC7 and IFIT1 mRNAs normalized to β-actin between PDCoV- and mock-infected cells was calculated based on the qPCR data. The iTRAQ ratio (PDCoV/Mock) obtained by MS analysis was simultaneously shown as a comparison. (C) Western blot (WB) analysis of the expression of ANAPC7 and IFIT1 proteins in IPEC-J2 cells upon PDCoV infection. IPEC-J2 cells were mock infected or infected with the PDCoV CHN-HN-1601 strain at an MOI of 0.1 TCID50/cell. At 24 hpi, the cells were harvested and processed for WB analysis using rabbit anti-ANAPC7, mouse anti-IFIT1 polyclonal antibodies and the mAb 1A3 specific for PDCoV. β-Actin was included as an internal loading control. T"}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T48","span":{"begin":714,"end":723},"obj":"Disease"},{"id":"T49","span":{"begin":785,"end":788},"obj":"Disease"}],"attributes":[{"id":"A48","pred":"mondo_id","subj":"T48","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"},{"id":"A49","pred":"mondo_id","subj":"T49","obj":"http://purl.obolibrary.org/obo/MONDO_0011527"}],"text":" strain at an MOI of 0.1 TCID50/cell and collected at 24 hpi. Total RNA was extracted and reverse-transcribed into cDNA for the subsequent analysis via qPCR. Fold-change values were calculated based on the 2–ΔΔCt method, using β-actin as the housekeeping gene. Error bars indicate the standard error of three independent experiments (Student’s t test; *p \u003c 0.05). (B) The relative ratio of ANAPC7 and IFIT1 mRNAs normalized to β-actin between PDCoV- and mock-infected cells was calculated based on the qPCR data. The iTRAQ ratio (PDCoV/Mock) obtained by MS analysis was simultaneously shown as a comparison. (C) Western blot (WB) analysis of the expression of ANAPC7 and IFIT1 proteins in IPEC-J2 cells upon PDCoV infection. IPEC-J2 cells were mock infected or infected with the PDCoV CHN-HN-1601 strain at an MOI of 0.1 TCID50/cell. At 24 hpi, the cells were harvested and processed for WB analysis using rabbit anti-ANAPC7, mouse anti-IFIT1 polyclonal antibodies and the mAb 1A3 specific for PDCoV. β-Actin was included as an internal loading control. T"}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T432","span":{"begin":32,"end":36},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T433","span":{"begin":255,"end":259},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T434","span":{"begin":346,"end":350},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T435","span":{"begin":365,"end":366},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T436","span":{"begin":468,"end":473},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T437","span":{"begin":554,"end":556},"obj":"http://purl.obolibrary.org/obo/CLO_0007874"},{"id":"T438","span":{"begin":594,"end":595},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T439","span":{"begin":697,"end":702},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T440","span":{"begin":733,"end":738},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T441","span":{"begin":789,"end":791},"obj":"http://purl.obolibrary.org/obo/CLO_0003788"},{"id":"T442","span":{"begin":828,"end":832},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T443","span":{"begin":849,"end":854},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T444","span":{"begin":926,"end":931},"obj":"http://purl.obolibrary.org/obo/CLO_0007836"}],"text":" strain at an MOI of 0.1 TCID50/cell and collected at 24 hpi. Total RNA was extracted and reverse-transcribed into cDNA for the subsequent analysis via qPCR. Fold-change values were calculated based on the 2–ΔΔCt method, using β-actin as the housekeeping gene. Error bars indicate the standard error of three independent experiments (Student’s t test; *p \u003c 0.05). (B) The relative ratio of ANAPC7 and IFIT1 mRNAs normalized to β-actin between PDCoV- and mock-infected cells was calculated based on the qPCR data. The iTRAQ ratio (PDCoV/Mock) obtained by MS analysis was simultaneously shown as a comparison. (C) Western blot (WB) analysis of the expression of ANAPC7 and IFIT1 proteins in IPEC-J2 cells upon PDCoV infection. IPEC-J2 cells were mock infected or infected with the PDCoV CHN-HN-1601 strain at an MOI of 0.1 TCID50/cell. At 24 hpi, the cells were harvested and processed for WB analysis using rabbit anti-ANAPC7, mouse anti-IFIT1 polyclonal antibodies and the mAb 1A3 specific for PDCoV. β-Actin was included as an internal loading control. T"}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T342","span":{"begin":554,"end":556},"obj":"Chemical"},{"id":"T343","span":{"begin":677,"end":685},"obj":"Chemical"}],"attributes":[{"id":"A342","pred":"chebi_id","subj":"T342","obj":"http://purl.obolibrary.org/obo/CHEBI_73613"},{"id":"A343","pred":"chebi_id","subj":"T343","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"}],"text":" strain at an MOI of 0.1 TCID50/cell and collected at 24 hpi. Total RNA was extracted and reverse-transcribed into cDNA for the subsequent analysis via qPCR. Fold-change values were calculated based on the 2–ΔΔCt method, using β-actin as the housekeeping gene. Error bars indicate the standard error of three independent experiments (Student’s t test; *p \u003c 0.05). (B) The relative ratio of ANAPC7 and IFIT1 mRNAs normalized to β-actin between PDCoV- and mock-infected cells was calculated based on the qPCR data. The iTRAQ ratio (PDCoV/Mock) obtained by MS analysis was simultaneously shown as a comparison. (C) Western blot (WB) analysis of the expression of ANAPC7 and IFIT1 proteins in IPEC-J2 cells upon PDCoV infection. IPEC-J2 cells were mock infected or infected with the PDCoV CHN-HN-1601 strain at an MOI of 0.1 TCID50/cell. At 24 hpi, the cells were harvested and processed for WB analysis using rabbit anti-ANAPC7, mouse anti-IFIT1 polyclonal antibodies and the mAb 1A3 specific for PDCoV. β-Actin was included as an internal loading control. T"}

{"project":"LitCovid-sentences","denotations":[{"id":"T246","span":{"begin":62,"end":157},"obj":"Sentence"},{"id":"T247","span":{"begin":158,"end":260},"obj":"Sentence"},{"id":"T248","span":{"begin":261,"end":512},"obj":"Sentence"},{"id":"T249","span":{"begin":513,"end":724},"obj":"Sentence"},{"id":"T250","span":{"begin":725,"end":833},"obj":"Sentence"},{"id":"T251","span":{"begin":834,"end":1053},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":" strain at an MOI of 0.1 TCID50/cell and collected at 24 hpi. Total RNA was extracted and reverse-transcribed into cDNA for the subsequent analysis via qPCR. Fold-change values were calculated based on the 2–ΔΔCt method, using β-actin as the housekeeping gene. Error bars indicate the standard error of three independent experiments (Student’s t test; *p \u003c 0.05). (B) The relative ratio of ANAPC7 and IFIT1 mRNAs normalized to β-actin between PDCoV- and mock-infected cells was calculated based on the qPCR data. The iTRAQ ratio (PDCoV/Mock) obtained by MS analysis was simultaneously shown as a comparison. (C) Western blot (WB) analysis of the expression of ANAPC7 and IFIT1 proteins in IPEC-J2 cells upon PDCoV infection. IPEC-J2 cells were mock infected or infected with the PDCoV CHN-HN-1601 strain at an MOI of 0.1 TCID50/cell. At 24 hpi, the cells were harvested and processed for WB analysis using rabbit anti-ANAPC7, mouse anti-IFIT1 polyclonal antibodies and the mAb 1A3 specific for PDCoV. β-Actin was included as an internal loading control. T"}

{"project":"LitCovid-PubTator","denotations":[{"id":"669","span":{"begin":227,"end":234},"obj":"Gene"},{"id":"670","span":{"begin":390,"end":396},"obj":"Gene"},{"id":"671","span":{"begin":401,"end":406},"obj":"Gene"},{"id":"672","span":{"begin":427,"end":434},"obj":"Gene"},{"id":"673","span":{"begin":660,"end":666},"obj":"Gene"},{"id":"674","span":{"begin":671,"end":676},"obj":"Gene"},{"id":"675","span":{"begin":918,"end":924},"obj":"Gene"},{"id":"676","span":{"begin":937,"end":942},"obj":"Gene"},{"id":"677","span":{"begin":1001,"end":1008},"obj":"Gene"},{"id":"679","span":{"begin":926,"end":931},"obj":"Species"},{"id":"684","span":{"begin":459,"end":467},"obj":"Disease"},{"id":"685","span":{"begin":714,"end":723},"obj":"Disease"},{"id":"686","span":{"begin":749,"end":757},"obj":"Disease"},{"id":"687","span":{"begin":761,"end":769},"obj":"Disease"},{"id":"688","span":{"begin":779,"end":796},"obj":"Disease"},{"id":"692","span":{"begin":689,"end":696},"obj":"CellLine"},{"id":"693","span":{"begin":725,"end":732},"obj":"CellLine"}],"attributes":[{"id":"A669","pred":"tao:has_database_id","subj":"669","obj":"Gene:728378"},{"id":"A670","pred":"tao:has_database_id","subj":"670","obj":"Gene:51434"},{"id":"A671","pred":"tao:has_database_id","subj":"671","obj":"Gene:3434"},{"id":"A672","pred":"tao:has_database_id","subj":"672","obj":"Gene:728378"},{"id":"A673","pred":"tao:has_database_id","subj":"673","obj":"Gene:100155743"},{"id":"A674","pred":"tao:has_database_id","subj":"674","obj":"Gene:100153038"},{"id":"A675","pred":"tao:has_database_id","subj":"675","obj":"Gene:56317"},{"id":"A676","pred":"tao:has_database_id","subj":"676","obj":"Gene:15957"},{"id":"A677","pred":"tao:has_database_id","subj":"677","obj":"Gene:728378"},{"id":"A679","pred":"tao:has_database_id","subj":"679","obj":"Tax:10090"},{"id":"A684","pred":"tao:has_database_id","subj":"684","obj":"MESH:D007239"},{"id":"A685","pred":"tao:has_database_id","subj":"685","obj":"MESH:D007239"},{"id":"A686","pred":"tao:has_database_id","subj":"686","obj":"MESH:D007239"},{"id":"A687","pred":"tao:has_database_id","subj":"687","obj":"MESH:D007239"},{"id":"A688","pred":"tao:has_database_id","subj":"688","obj":"MESH:C535301"},{"id":"A692","pred":"tao:has_database_id","subj":"692","obj":"CVCL:2246"},{"id":"A693","pred":"tao:has_database_id","subj":"693","obj":"CVCL:2246"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":" strain at an MOI of 0.1 TCID50/cell and collected at 24 hpi. Total RNA was extracted and reverse-transcribed into cDNA for the subsequent analysis via qPCR. Fold-change values were calculated based on the 2–ΔΔCt method, using β-actin as the housekeeping gene. Error bars indicate the standard error of three independent experiments (Student’s t test; *p \u003c 0.05). (B) The relative ratio of ANAPC7 and IFIT1 mRNAs normalized to β-actin between PDCoV- and mock-infected cells was calculated based on the qPCR data. The iTRAQ ratio (PDCoV/Mock) obtained by MS analysis was simultaneously shown as a comparison. (C) Western blot (WB) analysis of the expression of ANAPC7 and IFIT1 proteins in IPEC-J2 cells upon PDCoV infection. IPEC-J2 cells were mock infected or infected with the PDCoV CHN-HN-1601 strain at an MOI of 0.1 TCID50/cell. At 24 hpi, the cells were harvested and processed for WB analysis using rabbit anti-ANAPC7, mouse anti-IFIT1 polyclonal antibodies and the mAb 1A3 specific for PDCoV. β-Actin was included as an internal loading control. T"}