PMC:7640975 / 23928-24718
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7640975","sourcedb":"PMC","sourceid":"7640975","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7640975","text":"Figure 1 Proliferation of PDCoV in IPEC-J2 cells. (A) Morphological changes in IPEC-J2 cells infected with the PDCoV CHN-HN-1601 strain at an MOI of 0.1 TCID50/cell or mocked infected for 4, 8, 12, 24, and 36 h, respectively. Scale bars, 10 μm. (B) Confirmation of PDCoV proliferation in IPEC-J2 cells by immunofluorescence assays using the mAb 1A3 specific for PDCoV (α-N) and an Alexa Fluor 488-labeled goat antimouse IgG, and mock-infected cells at 24 h was used as a negative control. Cell nuclei were counterstained with DAPI (blue). Scale bars, 10 μm. (C) One-step growth curve of PDCoV in IPEC-J2 cells at the indicated time points following viral infection. The titer of virus was presented as TCID50/mL, and the data were recorded as means ± SD from three independent experiments.","divisions":[{"label":"label","span":{"begin":0,"end":8}}],"tracks":[{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T159","span":{"begin":44,"end":49},"obj":"Body_part"},{"id":"T160","span":{"begin":88,"end":93},"obj":"Body_part"},{"id":"T161","span":{"begin":161,"end":165},"obj":"Body_part"},{"id":"T162","span":{"begin":297,"end":302},"obj":"Body_part"},{"id":"T163","span":{"begin":421,"end":424},"obj":"Body_part"},{"id":"T164","span":{"begin":444,"end":449},"obj":"Body_part"},{"id":"T165","span":{"begin":490,"end":494},"obj":"Body_part"},{"id":"T166","span":{"begin":605,"end":610},"obj":"Body_part"}],"attributes":[{"id":"A159","pred":"fma_id","subj":"T159","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A160","pred":"fma_id","subj":"T160","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A161","pred":"fma_id","subj":"T161","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A162","pred":"fma_id","subj":"T162","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A163","pred":"fma_id","subj":"T163","obj":"http://purl.org/sig/ont/fma/fma62872"},{"id":"A164","pred":"fma_id","subj":"T164","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A165","pred":"fma_id","subj":"T165","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A166","pred":"fma_id","subj":"T166","obj":"http://purl.org/sig/ont/fma/fma68646"},{"subj":"T159","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T160","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T161","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T162","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T163","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T164","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T165","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T166","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T22","span":{"begin":227,"end":232},"obj":"Body_part"},{"id":"T23","span":{"begin":540,"end":545},"obj":"Body_part"}],"attributes":[{"id":"A22","pred":"uberon_id","subj":"T22","obj":"http://purl.obolibrary.org/obo/UBERON_0002542"},{"id":"A23","pred":"uberon_id","subj":"T23","obj":"http://purl.obolibrary.org/obo/UBERON_0002542"},{"subj":"T22","pred":"source","obj":"LitCovid-PD-UBERON"},{"subj":"T23","pred":"source","obj":"LitCovid-PD-UBERON"}]},{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T29","span":{"begin":118,"end":121},"obj":"Disease"},{"id":"T30","span":{"begin":650,"end":665},"obj":"Disease"},{"id":"T31","span":{"begin":656,"end":665},"obj":"Disease"}],"attributes":[{"id":"A29","pred":"mondo_id","subj":"T29","obj":"http://purl.obolibrary.org/obo/MONDO_0011527"},{"id":"A30","pred":"mondo_id","subj":"T30","obj":"http://purl.obolibrary.org/obo/MONDO_0005108"},{"id":"A31","pred":"mondo_id","subj":"T31","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"},{"subj":"T29","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T30","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T31","pred":"source","obj":"LitCovid-PD-MONDO"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T294","span":{"begin":44,"end":49},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T295","span":{"begin":52,"end":53},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T296","span":{"begin":88,"end":93},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T297","span":{"begin":122,"end":124},"obj":"http://purl.obolibrary.org/obo/CLO_0003788"},{"id":"T298","span":{"begin":161,"end":165},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T299","span":{"begin":189,"end":193},"obj":"http://purl.obolibrary.org/obo/CLO_0001382"},{"id":"T300","span":{"begin":207,"end":209},"obj":"http://purl.obolibrary.org/obo/CLO_0001313"},{"id":"T301","span":{"begin":243,"end":249},"obj":"http://purl.obolibrary.org/obo/PR_000010213"},{"id":"T302","span":{"begin":297,"end":302},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T303","span":{"begin":398,"end":405},"obj":"http://purl.obolibrary.org/obo/CLO_0007225"},{"id":"T304","span":{"begin":406,"end":410},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9925"},{"id":"T305","span":{"begin":444,"end":449},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T306","span":{"begin":470,"end":471},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T307","span":{"begin":490,"end":494},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T308","span":{"begin":605,"end":610},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T309","span":{"begin":680,"end":685},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"}],"attributes":[{"subj":"T294","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T295","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T296","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T297","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T298","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T299","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T300","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T301","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T302","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T303","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T304","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T305","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T306","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T307","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T308","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T309","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T155","span":{"begin":382,"end":397},"obj":"Chemical"},{"id":"T156","span":{"begin":388,"end":393},"obj":"Chemical"},{"id":"T157","span":{"begin":495,"end":501},"obj":"Chemical"},{"id":"T158","span":{"begin":527,"end":531},"obj":"Chemical"},{"id":"T159","span":{"begin":752,"end":754},"obj":"Chemical"}],"attributes":[{"id":"A155","pred":"chebi_id","subj":"T155","obj":"http://purl.obolibrary.org/obo/CHEBI_52661"},{"id":"A156","pred":"chebi_id","subj":"T156","obj":"http://purl.obolibrary.org/obo/CHEBI_24061"},{"id":"A157","pred":"chebi_id","subj":"T157","obj":"http://purl.obolibrary.org/obo/CHEBI_33252"},{"id":"A158","pred":"chebi_id","subj":"T158","obj":"http://purl.obolibrary.org/obo/CHEBI_51231"},{"id":"A159","pred":"chebi_id","subj":"T159","obj":"http://purl.obolibrary.org/obo/CHEBI_74807"},{"subj":"T155","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T156","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T157","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T158","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T159","pred":"source","obj":"LitCovid-PD-CHEBI"}]},{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T35","span":{"begin":572,"end":578},"obj":"http://purl.obolibrary.org/obo/GO_0040007"},{"id":"T36","span":{"begin":650,"end":665},"obj":"http://purl.obolibrary.org/obo/GO_0016032"}],"attributes":[{"subj":"T35","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T36","pred":"source","obj":"LitCovid-PD-GO-BP"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T136","span":{"begin":0,"end":226},"obj":"Sentence"},{"id":"T137","span":{"begin":227,"end":489},"obj":"Sentence"},{"id":"T138","span":{"begin":490,"end":539},"obj":"Sentence"},{"id":"T139","span":{"begin":540,"end":666},"obj":"Sentence"},{"id":"T140","span":{"begin":667,"end":790},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T136","pred":"source","obj":"LitCovid-sentences"},{"subj":"T137","pred":"source","obj":"LitCovid-sentences"},{"subj":"T138","pred":"source","obj":"LitCovid-sentences"},{"subj":"T139","pred":"source","obj":"LitCovid-sentences"},{"subj":"T140","pred":"source","obj":"LitCovid-sentences"}]},{"project":"LitCovid-PubTator","denotations":[{"id":"382","span":{"begin":370,"end":373},"obj":"Chemical"},{"id":"383","span":{"begin":382,"end":397},"obj":"Chemical"},{"id":"384","span":{"begin":527,"end":531},"obj":"Chemical"},{"id":"385","span":{"begin":94,"end":102},"obj":"Disease"},{"id":"386","span":{"begin":112,"end":129},"obj":"Disease"},{"id":"387","span":{"begin":176,"end":184},"obj":"Disease"},{"id":"388","span":{"begin":435,"end":443},"obj":"Disease"},{"id":"389","span":{"begin":650,"end":665},"obj":"Disease"},{"id":"390","span":{"begin":36,"end":43},"obj":"CellLine"},{"id":"391","span":{"begin":80,"end":87},"obj":"CellLine"},{"id":"392","span":{"begin":289,"end":296},"obj":"CellLine"},{"id":"393","span":{"begin":597,"end":604},"obj":"CellLine"}],"attributes":[{"id":"A384","pred":"tao:has_database_id","subj":"384","obj":"MESH:C007293"},{"id":"A385","pred":"tao:has_database_id","subj":"385","obj":"MESH:D007239"},{"id":"A386","pred":"tao:has_database_id","subj":"386","obj":"MESH:C535301"},{"id":"A387","pred":"tao:has_database_id","subj":"387","obj":"MESH:D007239"},{"id":"A388","pred":"tao:has_database_id","subj":"388","obj":"MESH:D007239"},{"id":"A389","pred":"tao:has_database_id","subj":"389","obj":"MESH:D001102"},{"id":"A390","pred":"tao:has_database_id","subj":"390","obj":"CVCL:2246"},{"id":"A391","pred":"tao:has_database_id","subj":"391","obj":"CVCL:2246"},{"id":"A392","pred":"tao:has_database_id","subj":"392","obj":"CVCL:2246"},{"id":"A393","pred":"tao:has_database_id","subj":"393","obj":"CVCL:2246"},{"subj":"382","pred":"source","obj":"LitCovid-PubTator"},{"subj":"383","pred":"source","obj":"LitCovid-PubTator"},{"subj":"384","pred":"source","obj":"LitCovid-PubTator"},{"subj":"385","pred":"source","obj":"LitCovid-PubTator"},{"subj":"386","pred":"source","obj":"LitCovid-PubTator"},{"subj":"387","pred":"source","obj":"LitCovid-PubTator"},{"subj":"388","pred":"source","obj":"LitCovid-PubTator"},{"subj":"389","pred":"source","obj":"LitCovid-PubTator"},{"subj":"390","pred":"source","obj":"LitCovid-PubTator"},{"subj":"391","pred":"source","obj":"LitCovid-PubTator"},{"subj":"392","pred":"source","obj":"LitCovid-PubTator"},{"subj":"393","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PD-FMA-UBERON","color":"#b793ec","default":true},{"id":"LitCovid-PD-UBERON","color":"#93ec9d"},{"id":"LitCovid-PD-MONDO","color":"#ec93a2"},{"id":"LitCovid-PD-CLO","color":"#93bcec"},{"id":"LitCovid-PD-CHEBI","color":"#d6ec93"},{"id":"LitCovid-PD-GO-BP","color":"#e893ec"},{"id":"LitCovid-sentences","color":"#93ecce"},{"id":"LitCovid-PubTator","color":"#ecb493"}]}]}}