PMC:7640975 / 231-1286 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T4","span":{"begin":35,"end":51},"obj":"Body_part"},{"id":"T5","span":{"begin":52,"end":68},"obj":"Body_part"},{"id":"T6","span":{"begin":63,"end":68},"obj":"Body_part"},{"id":"T7","span":{"begin":99,"end":104},"obj":"Body_part"},{"id":"T8","span":{"begin":341,"end":349},"obj":"Body_part"},{"id":"T9","span":{"begin":383,"end":388},"obj":"Body_part"},{"id":"T10","span":{"begin":456,"end":464},"obj":"Body_part"},{"id":"T11","span":{"begin":748,"end":755},"obj":"Body_part"}],"attributes":[{"id":"A4","pred":"fma_id","subj":"T4","obj":"http://purl.org/sig/ont/fma/fma7200"},{"id":"A5","pred":"fma_id","subj":"T5","obj":"http://purl.org/sig/ont/fma/fma66768"},{"id":"A6","pred":"fma_id","subj":"T6","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A7","pred":"fma_id","subj":"T7","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A8","pred":"fma_id","subj":"T8","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A9","pred":"fma_id","subj":"T9","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A10","pred":"fma_id","subj":"T10","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A11","pred":"fma_id","subj":"T11","obj":"http://purl.org/sig/ont/fma/fma67257"}],"text":"iated with swine diarrhea. Porcine small intestinal epithelial cells (IPEC) are the primary target cells of PDCoV infection in vivo. Here, isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled to liquid chromatography–tandem mass spectrometry (LC-MS/MS) was used to quantitatively identify differentially expressed proteins (DEPs) in PDCoV-infected IPEC-J2 cells. A total of 78 DEPs, including 23 upregulated and 55 downregulated proteins, were identified at 24 h postinfection. The data are available via ProteomeXchange with identifier PXD019975. To ensure reliability of the proteomics data, two randomly selected DEPs, the downregulated anaphase-promoting complex subunit 7 (ANAPC7) and upregulated interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), were verified by real-time PCR and Western blot, and the results of which indicate that the proteomics data were reliable and valid. Bioinformatics analyses, including GO, COG, KEGG, and STRING, further demonstrated that a majority of the DEPs are involved "}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T1","span":{"begin":35,"end":51},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0002108"}],"text":"iated with swine diarrhea. Porcine small intestinal epithelial cells (IPEC) are the primary target cells of PDCoV infection in vivo. Here, isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled to liquid chromatography–tandem mass spectrometry (LC-MS/MS) was used to quantitatively identify differentially expressed proteins (DEPs) in PDCoV-infected IPEC-J2 cells. A total of 78 DEPs, including 23 upregulated and 55 downregulated proteins, were identified at 24 h postinfection. The data are available via ProteomeXchange with identifier PXD019975. To ensure reliability of the proteomics data, two randomly selected DEPs, the downregulated anaphase-promoting complex subunit 7 (ANAPC7) and upregulated interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), were verified by real-time PCR and Western blot, and the results of which indicate that the proteomics data were reliable and valid. Bioinformatics analyses, including GO, COG, KEGG, and STRING, further demonstrated that a majority of the DEPs are involved "}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T1","span":{"begin":17,"end":25},"obj":"Disease"},{"id":"T2","span":{"begin":114,"end":123},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0001673"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"}],"text":"iated with swine diarrhea. Porcine small intestinal epithelial cells (IPEC) are the primary target cells of PDCoV infection in vivo. Here, isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled to liquid chromatography–tandem mass spectrometry (LC-MS/MS) was used to quantitatively identify differentially expressed proteins (DEPs) in PDCoV-infected IPEC-J2 cells. A total of 78 DEPs, including 23 upregulated and 55 downregulated proteins, were identified at 24 h postinfection. The data are available via ProteomeXchange with identifier PXD019975. To ensure reliability of the proteomics data, two randomly selected DEPs, the downregulated anaphase-promoting complex subunit 7 (ANAPC7) and upregulated interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), were verified by real-time PCR and Western blot, and the results of which indicate that the proteomics data were reliable and valid. Bioinformatics analyses, including GO, COG, KEGG, and STRING, further demonstrated that a majority of the DEPs are involved "}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T8","span":{"begin":35,"end":51},"obj":"http://purl.obolibrary.org/obo/UBERON_0002108"},{"id":"T9","span":{"begin":52,"end":62},"obj":"http://purl.obolibrary.org/obo/CL_0000066"},{"id":"T10","span":{"begin":63,"end":68},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T11","span":{"begin":99,"end":104},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T12","span":{"begin":202,"end":210},"obj":"http://purl.obolibrary.org/obo/CLO_0007225"},{"id":"T13","span":{"begin":270,"end":272},"obj":"http://purl.obolibrary.org/obo/CL_0000453"},{"id":"T14","span":{"begin":273,"end":275},"obj":"http://purl.obolibrary.org/obo/CLO_0007874"},{"id":"T15","span":{"begin":276,"end":278},"obj":"http://purl.obolibrary.org/obo/CLO_0007874"},{"id":"T16","span":{"begin":383,"end":388},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T17","span":{"begin":390,"end":391},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T18","span":{"begin":1019,"end":1020},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"iated with swine diarrhea. Porcine small intestinal epithelial cells (IPEC) are the primary target cells of PDCoV infection in vivo. Here, isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled to liquid chromatography–tandem mass spectrometry (LC-MS/MS) was used to quantitatively identify differentially expressed proteins (DEPs) in PDCoV-infected IPEC-J2 cells. A total of 78 DEPs, including 23 upregulated and 55 downregulated proteins, were identified at 24 h postinfection. The data are available via ProteomeXchange with identifier PXD019975. To ensure reliability of the proteomics data, two randomly selected DEPs, the downregulated anaphase-promoting complex subunit 7 (ANAPC7) and upregulated interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), were verified by real-time PCR and Western blot, and the results of which indicate that the proteomics data were reliable and valid. Bioinformatics analyses, including GO, COG, KEGG, and STRING, further demonstrated that a majority of the DEPs are involved "}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T4","span":{"begin":270,"end":272},"obj":"Chemical"},{"id":"T5","span":{"begin":273,"end":275},"obj":"Chemical"},{"id":"T6","span":{"begin":276,"end":278},"obj":"Chemical"},{"id":"T7","span":{"begin":341,"end":349},"obj":"Chemical"},{"id":"T8","span":{"begin":456,"end":464},"obj":"Chemical"},{"id":"T9","span":{"begin":729,"end":739},"obj":"Chemical"},{"id":"T10","span":{"begin":748,"end":755},"obj":"Chemical"}],"attributes":[{"id":"A4","pred":"chebi_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CHEBI_73582"},{"id":"A5","pred":"chebi_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CHEBI_73613"},{"id":"A6","pred":"chebi_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/CHEBI_73613"},{"id":"A7","pred":"chebi_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A8","pred":"chebi_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A9","pred":"chebi_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/CHEBI_52999"},{"id":"A10","pred":"chebi_id","subj":"T10","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"}],"text":"iated with swine diarrhea. Porcine small intestinal epithelial cells (IPEC) are the primary target cells of PDCoV infection in vivo. Here, isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled to liquid chromatography–tandem mass spectrometry (LC-MS/MS) was used to quantitatively identify differentially expressed proteins (DEPs) in PDCoV-infected IPEC-J2 cells. A total of 78 DEPs, including 23 upregulated and 55 downregulated proteins, were identified at 24 h postinfection. The data are available via ProteomeXchange with identifier PXD019975. To ensure reliability of the proteomics data, two randomly selected DEPs, the downregulated anaphase-promoting complex subunit 7 (ANAPC7) and upregulated interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), were verified by real-time PCR and Western blot, and the results of which indicate that the proteomics data were reliable and valid. Bioinformatics analyses, including GO, COG, KEGG, and STRING, further demonstrated that a majority of the DEPs are involved "}

{"project":"LitCovid-PD-HP","denotations":[{"id":"T1","span":{"begin":17,"end":25},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/HP_0002014"}],"text":"iated with swine diarrhea. Porcine small intestinal epithelial cells (IPEC) are the primary target cells of PDCoV infection in vivo. Here, isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled to liquid chromatography–tandem mass spectrometry (LC-MS/MS) was used to quantitatively identify differentially expressed proteins (DEPs) in PDCoV-infected IPEC-J2 cells. A total of 78 DEPs, including 23 upregulated and 55 downregulated proteins, were identified at 24 h postinfection. The data are available via ProteomeXchange with identifier PXD019975. To ensure reliability of the proteomics data, two randomly selected DEPs, the downregulated anaphase-promoting complex subunit 7 (ANAPC7) and upregulated interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), were verified by real-time PCR and Western blot, and the results of which indicate that the proteomics data were reliable and valid. Bioinformatics analyses, including GO, COG, KEGG, and STRING, further demonstrated that a majority of the DEPs are involved "}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T1","span":{"begin":667,"end":675},"obj":"http://purl.obolibrary.org/obo/GO_0051322"}],"text":"iated with swine diarrhea. Porcine small intestinal epithelial cells (IPEC) are the primary target cells of PDCoV infection in vivo. Here, isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled to liquid chromatography–tandem mass spectrometry (LC-MS/MS) was used to quantitatively identify differentially expressed proteins (DEPs) in PDCoV-infected IPEC-J2 cells. A total of 78 DEPs, including 23 upregulated and 55 downregulated proteins, were identified at 24 h postinfection. The data are available via ProteomeXchange with identifier PXD019975. To ensure reliability of the proteomics data, two randomly selected DEPs, the downregulated anaphase-promoting complex subunit 7 (ANAPC7) and upregulated interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), were verified by real-time PCR and Western blot, and the results of which indicate that the proteomics data were reliable and valid. Bioinformatics analyses, including GO, COG, KEGG, and STRING, further demonstrated that a majority of the DEPs are involved "}

{"project":"LitCovid-sentences","denotations":[{"id":"T4","span":{"begin":27,"end":132},"obj":"Sentence"},{"id":"T5","span":{"begin":133,"end":389},"obj":"Sentence"},{"id":"T6","span":{"begin":390,"end":504},"obj":"Sentence"},{"id":"T7","span":{"begin":505,"end":574},"obj":"Sentence"},{"id":"T8","span":{"begin":575,"end":930},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"iated with swine diarrhea. Porcine small intestinal epithelial cells (IPEC) are the primary target cells of PDCoV infection in vivo. Here, isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled to liquid chromatography–tandem mass spectrometry (LC-MS/MS) was used to quantitatively identify differentially expressed proteins (DEPs) in PDCoV-infected IPEC-J2 cells. A total of 78 DEPs, including 23 upregulated and 55 downregulated proteins, were identified at 24 h postinfection. The data are available via ProteomeXchange with identifier PXD019975. To ensure reliability of the proteomics data, two randomly selected DEPs, the downregulated anaphase-promoting complex subunit 7 (ANAPC7) and upregulated interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), were verified by real-time PCR and Western blot, and the results of which indicate that the proteomics data were reliable and valid. Bioinformatics analyses, including GO, COG, KEGG, and STRING, further demonstrated that a majority of the DEPs are involved "}

{"project":"LitCovid-PubTator","denotations":[{"id":"21","span":{"begin":667,"end":703},"obj":"Gene"},{"id":"22","span":{"begin":705,"end":711},"obj":"Gene"},{"id":"23","span":{"begin":729,"end":788},"obj":"Gene"},{"id":"24","span":{"begin":790,"end":795},"obj":"Gene"},{"id":"29","span":{"begin":11,"end":16},"obj":"Species"},{"id":"30","span":{"begin":27,"end":34},"obj":"Species"},{"id":"31","span":{"begin":17,"end":25},"obj":"Disease"},{"id":"32","span":{"begin":114,"end":123},"obj":"Disease"},{"id":"33","span":{"begin":360,"end":374},"obj":"Disease"},{"id":"35","span":{"begin":375,"end":382},"obj":"CellLine"}],"attributes":[{"id":"A21","pred":"tao:has_database_id","subj":"21","obj":"Gene:51434"},{"id":"A22","pred":"tao:has_database_id","subj":"22","obj":"Gene:51434"},{"id":"A23","pred":"tao:has_database_id","subj":"23","obj":"Gene:3434"},{"id":"A24","pred":"tao:has_database_id","subj":"24","obj":"Gene:3434"},{"id":"A29","pred":"tao:has_database_id","subj":"29","obj":"Tax:9823"},{"id":"A30","pred":"tao:has_database_id","subj":"30","obj":"Tax:28344"},{"id":"A31","pred":"tao:has_database_id","subj":"31","obj":"MESH:D003967"},{"id":"A32","pred":"tao:has_database_id","subj":"32","obj":"MESH:D007239"},{"id":"A33","pred":"tao:has_database_id","subj":"33","obj":"MESH:D007239"},{"id":"A35","pred":"tao:has_database_id","subj":"35","obj":"CVCL:2246"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"iated with swine diarrhea. Porcine small intestinal epithelial cells (IPEC) are the primary target cells of PDCoV infection in vivo. Here, isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled to liquid chromatography–tandem mass spectrometry (LC-MS/MS) was used to quantitatively identify differentially expressed proteins (DEPs) in PDCoV-infected IPEC-J2 cells. A total of 78 DEPs, including 23 upregulated and 55 downregulated proteins, were identified at 24 h postinfection. The data are available via ProteomeXchange with identifier PXD019975. To ensure reliability of the proteomics data, two randomly selected DEPs, the downregulated anaphase-promoting complex subunit 7 (ANAPC7) and upregulated interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), were verified by real-time PCR and Western blot, and the results of which indicate that the proteomics data were reliable and valid. Bioinformatics analyses, including GO, COG, KEGG, and STRING, further demonstrated that a majority of the DEPs are involved "}