PMC:7640975 / 19181-20275 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T126","span":{"begin":95,"end":102},"obj":"Body_part"},{"id":"T127","span":{"begin":129,"end":134},"obj":"Body_part"},{"id":"T128","span":{"begin":183,"end":191},"obj":"Body_part"},{"id":"T129","span":{"begin":250,"end":255},"obj":"Body_part"},{"id":"T130","span":{"begin":281,"end":288},"obj":"Body_part"},{"id":"T131","span":{"begin":476,"end":480},"obj":"Body_part"},{"id":"T132","span":{"begin":563,"end":567},"obj":"Body_part"},{"id":"T133","span":{"begin":667,"end":672},"obj":"Body_part"},{"id":"T134","span":{"begin":879,"end":886},"obj":"Body_part"}],"attributes":[{"id":"A126","pred":"fma_id","subj":"T126","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A127","pred":"fma_id","subj":"T127","obj":"http://purl.org/sig/ont/fma/fma67843"},{"id":"A128","pred":"fma_id","subj":"T128","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A129","pred":"fma_id","subj":"T129","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A130","pred":"fma_id","subj":"T130","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A131","pred":"fma_id","subj":"T131","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A132","pred":"fma_id","subj":"T132","obj":"http://purl.org/sig/ont/fma/fma62100"},{"id":"A133","pred":"fma_id","subj":"T133","obj":"http://purl.org/sig/ont/fma/fma67843"},{"id":"A134","pred":"fma_id","subj":"T134","obj":"http://purl.org/sig/ont/fma/fma67257"}],"text":"To further validate the identified DEPs, Western blot was performed to detect ANAPC7 and IFIT1 protein expression levels using β-actin as an internal control. Briefly, total cellular proteins were extracted from both mock- and PDCoV-infected IPEC-J2 cells at 24 hpi. The resulting protein samples were separated on 12% SDS-PAGE gels and transferred onto 0.22 μm poly(vinylidene fluoride) (PVDF) membranes (Millipore, Bedford, MA, USA) using a semidry electrophoretic transfer cell (Bio-Rad, Hercules, CA, USA). The membranes were blocked with 5% (w/v) nonfat dry milk overnight at 4 °C and then probed with either anti-ANAPC7 (1:1000), anti-IFIT1 (1:1000), or anti-β-actin (1:5000) primary antibodies at 37 °C for 1 h. After thorough washing with PBST, the membranes were incubated with the corresponding HRP-conjugated secondary antibodies (1:8000) for 1 h at 37 °C. The target protein blots on the membranes were developed with an enhanced chemiluminescence detection kit (Thermo Fisher Scientific), and images were taken using a ProteinSimple FluorChem E image system (Santa Clara, CA, USA)."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T21","span":{"begin":563,"end":567},"obj":"Body_part"}],"attributes":[{"id":"A21","pred":"uberon_id","subj":"T21","obj":"http://purl.obolibrary.org/obo/UBERON_0001913"}],"text":"To further validate the identified DEPs, Western blot was performed to detect ANAPC7 and IFIT1 protein expression levels using β-actin as an internal control. Briefly, total cellular proteins were extracted from both mock- and PDCoV-infected IPEC-J2 cells at 24 hpi. The resulting protein samples were separated on 12% SDS-PAGE gels and transferred onto 0.22 μm poly(vinylidene fluoride) (PVDF) membranes (Millipore, Bedford, MA, USA) using a semidry electrophoretic transfer cell (Bio-Rad, Hercules, CA, USA). The membranes were blocked with 5% (w/v) nonfat dry milk overnight at 4 °C and then probed with either anti-ANAPC7 (1:1000), anti-IFIT1 (1:1000), or anti-β-actin (1:5000) primary antibodies at 37 °C for 1 h. After thorough washing with PBST, the membranes were incubated with the corresponding HRP-conjugated secondary antibodies (1:8000) for 1 h at 37 °C. The target protein blots on the membranes were developed with an enhanced chemiluminescence detection kit (Thermo Fisher Scientific), and images were taken using a ProteinSimple FluorChem E image system (Santa Clara, CA, USA)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T242","span":{"begin":250,"end":255},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T243","span":{"begin":395,"end":404},"obj":"http://purl.obolibrary.org/obo/UBERON_0000158"},{"id":"T244","span":{"begin":441,"end":442},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T245","span":{"begin":476,"end":480},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T246","span":{"begin":515,"end":524},"obj":"http://purl.obolibrary.org/obo/UBERON_0000158"},{"id":"T247","span":{"begin":547,"end":550},"obj":"http://purl.obolibrary.org/obo/CLO_0050644"},{"id":"T248","span":{"begin":581,"end":585},"obj":"http://purl.obolibrary.org/obo/CLO_0001387"},{"id":"T249","span":{"begin":757,"end":766},"obj":"http://purl.obolibrary.org/obo/UBERON_0000158"},{"id":"T250","span":{"begin":900,"end":909},"obj":"http://purl.obolibrary.org/obo/UBERON_0000158"},{"id":"T251","span":{"begin":1030,"end":1031},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"To further validate the identified DEPs, Western blot was performed to detect ANAPC7 and IFIT1 protein expression levels using β-actin as an internal control. Briefly, total cellular proteins were extracted from both mock- and PDCoV-infected IPEC-J2 cells at 24 hpi. The resulting protein samples were separated on 12% SDS-PAGE gels and transferred onto 0.22 μm poly(vinylidene fluoride) (PVDF) membranes (Millipore, Bedford, MA, USA) using a semidry electrophoretic transfer cell (Bio-Rad, Hercules, CA, USA). The membranes were blocked with 5% (w/v) nonfat dry milk overnight at 4 °C and then probed with either anti-ANAPC7 (1:1000), anti-IFIT1 (1:1000), or anti-β-actin (1:5000) primary antibodies at 37 °C for 1 h. After thorough washing with PBST, the membranes were incubated with the corresponding HRP-conjugated secondary antibodies (1:8000) for 1 h at 37 °C. The target protein blots on the membranes were developed with an enhanced chemiluminescence detection kit (Thermo Fisher Scientific), and images were taken using a ProteinSimple FluorChem E image system (Santa Clara, CA, USA)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T134","span":{"begin":95,"end":102},"obj":"Chemical"},{"id":"T135","span":{"begin":183,"end":191},"obj":"Chemical"},{"id":"T136","span":{"begin":281,"end":288},"obj":"Chemical"},{"id":"T137","span":{"begin":319,"end":322},"obj":"Chemical"},{"id":"T138","span":{"begin":362,"end":387},"obj":"Chemical"},{"id":"T139","span":{"begin":367,"end":377},"obj":"Chemical"},{"id":"T140","span":{"begin":378,"end":386},"obj":"Chemical"},{"id":"T141","span":{"begin":426,"end":428},"obj":"Chemical"},{"id":"T144","span":{"begin":879,"end":886},"obj":"Chemical"}],"attributes":[{"id":"A134","pred":"chebi_id","subj":"T134","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A135","pred":"chebi_id","subj":"T135","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A136","pred":"chebi_id","subj":"T136","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A137","pred":"chebi_id","subj":"T137","obj":"http://purl.obolibrary.org/obo/CHEBI_8984"},{"id":"A138","pred":"chebi_id","subj":"T138","obj":"http://purl.obolibrary.org/obo/CHEBI_53250"},{"id":"A139","pred":"chebi_id","subj":"T139","obj":"http://purl.obolibrary.org/obo/CHEBI_29854"},{"id":"A140","pred":"chebi_id","subj":"T140","obj":"http://purl.obolibrary.org/obo/CHEBI_17051"},{"id":"A141","pred":"chebi_id","subj":"T141","obj":"http://purl.obolibrary.org/obo/CHEBI_474859"},{"id":"A142","pred":"chebi_id","subj":"T141","obj":"http://purl.obolibrary.org/obo/CHEBI_73610"},{"id":"A143","pred":"chebi_id","subj":"T141","obj":"http://purl.obolibrary.org/obo/CHEBI_90325"},{"id":"A144","pred":"chebi_id","subj":"T144","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"}],"text":"To further validate the identified DEPs, Western blot was performed to detect ANAPC7 and IFIT1 protein expression levels using β-actin as an internal control. Briefly, total cellular proteins were extracted from both mock- and PDCoV-infected IPEC-J2 cells at 24 hpi. The resulting protein samples were separated on 12% SDS-PAGE gels and transferred onto 0.22 μm poly(vinylidene fluoride) (PVDF) membranes (Millipore, Bedford, MA, USA) using a semidry electrophoretic transfer cell (Bio-Rad, Hercules, CA, USA). The membranes were blocked with 5% (w/v) nonfat dry milk overnight at 4 °C and then probed with either anti-ANAPC7 (1:1000), anti-IFIT1 (1:1000), or anti-β-actin (1:5000) primary antibodies at 37 °C for 1 h. After thorough washing with PBST, the membranes were incubated with the corresponding HRP-conjugated secondary antibodies (1:8000) for 1 h at 37 °C. The target protein blots on the membranes were developed with an enhanced chemiluminescence detection kit (Thermo Fisher Scientific), and images were taken using a ProteinSimple FluorChem E image system (Santa Clara, CA, USA)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T108","span":{"begin":0,"end":158},"obj":"Sentence"},{"id":"T109","span":{"begin":159,"end":266},"obj":"Sentence"},{"id":"T110","span":{"begin":267,"end":510},"obj":"Sentence"},{"id":"T111","span":{"begin":511,"end":718},"obj":"Sentence"},{"id":"T112","span":{"begin":719,"end":867},"obj":"Sentence"},{"id":"T113","span":{"begin":868,"end":1094},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"To further validate the identified DEPs, Western blot was performed to detect ANAPC7 and IFIT1 protein expression levels using β-actin as an internal control. Briefly, total cellular proteins were extracted from both mock- and PDCoV-infected IPEC-J2 cells at 24 hpi. The resulting protein samples were separated on 12% SDS-PAGE gels and transferred onto 0.22 μm poly(vinylidene fluoride) (PVDF) membranes (Millipore, Bedford, MA, USA) using a semidry electrophoretic transfer cell (Bio-Rad, Hercules, CA, USA). The membranes were blocked with 5% (w/v) nonfat dry milk overnight at 4 °C and then probed with either anti-ANAPC7 (1:1000), anti-IFIT1 (1:1000), or anti-β-actin (1:5000) primary antibodies at 37 °C for 1 h. After thorough washing with PBST, the membranes were incubated with the corresponding HRP-conjugated secondary antibodies (1:8000) for 1 h at 37 °C. The target protein blots on the membranes were developed with an enhanced chemiluminescence detection kit (Thermo Fisher Scientific), and images were taken using a ProteinSimple FluorChem E image system (Santa Clara, CA, USA)."}

{"project":"LitCovid-PubTator","denotations":[{"id":"326","span":{"begin":78,"end":84},"obj":"Gene"},{"id":"327","span":{"begin":89,"end":94},"obj":"Gene"},{"id":"328","span":{"begin":127,"end":134},"obj":"Gene"},{"id":"329","span":{"begin":486,"end":489},"obj":"Gene"},{"id":"330","span":{"begin":619,"end":625},"obj":"Gene"},{"id":"331","span":{"begin":641,"end":646},"obj":"Gene"},{"id":"332","span":{"begin":970,"end":973},"obj":"Gene"},{"id":"333","span":{"begin":665,"end":672},"obj":"Gene"},{"id":"334","span":{"begin":319,"end":322},"obj":"Chemical"},{"id":"335","span":{"begin":362,"end":387},"obj":"Chemical"},{"id":"336","span":{"begin":389,"end":393},"obj":"Chemical"},{"id":"337","span":{"begin":227,"end":241},"obj":"Disease"},{"id":"338","span":{"begin":559,"end":567},"obj":"Disease"},{"id":"339","span":{"begin":242,"end":249},"obj":"CellLine"}],"attributes":[{"id":"A326","pred":"tao:has_database_id","subj":"326","obj":"Gene:51434"},{"id":"A327","pred":"tao:has_database_id","subj":"327","obj":"Gene:3434"},{"id":"A328","pred":"tao:has_database_id","subj":"328","obj":"Gene:728378"},{"id":"A329","pred":"tao:has_database_id","subj":"329","obj":"Gene:6236"},{"id":"A330","pred":"tao:has_database_id","subj":"330","obj":"Gene:51434"},{"id":"A331","pred":"tao:has_database_id","subj":"331","obj":"Gene:3434"},{"id":"A332","pred":"tao:has_database_id","subj":"332","obj":"Gene:3815"},{"id":"A333","pred":"tao:has_database_id","subj":"333","obj":"Gene:728378"},{"id":"A334","pred":"tao:has_database_id","subj":"334","obj":"MESH:D012967"},{"id":"A335","pred":"tao:has_database_id","subj":"335","obj":"MESH:C024865"},{"id":"A336","pred":"tao:has_database_id","subj":"336","obj":"MESH:C024865"},{"id":"A337","pred":"tao:has_database_id","subj":"337","obj":"MESH:D007239"},{"id":"A338","pred":"tao:has_database_id","subj":"338","obj":"MESH:D016269"},{"id":"A339","pred":"tao:has_database_id","subj":"339","obj":"CVCL:2246"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"To further validate the identified DEPs, Western blot was performed to detect ANAPC7 and IFIT1 protein expression levels using β-actin as an internal control. Briefly, total cellular proteins were extracted from both mock- and PDCoV-infected IPEC-J2 cells at 24 hpi. The resulting protein samples were separated on 12% SDS-PAGE gels and transferred onto 0.22 μm poly(vinylidene fluoride) (PVDF) membranes (Millipore, Bedford, MA, USA) using a semidry electrophoretic transfer cell (Bio-Rad, Hercules, CA, USA). The membranes were blocked with 5% (w/v) nonfat dry milk overnight at 4 °C and then probed with either anti-ANAPC7 (1:1000), anti-IFIT1 (1:1000), or anti-β-actin (1:5000) primary antibodies at 37 °C for 1 h. After thorough washing with PBST, the membranes were incubated with the corresponding HRP-conjugated secondary antibodies (1:8000) for 1 h at 37 °C. The target protein blots on the membranes were developed with an enhanced chemiluminescence detection kit (Thermo Fisher Scientific), and images were taken using a ProteinSimple FluorChem E image system (Santa Clara, CA, USA)."}