PMC:7640975 / 19181-20275
Annnotations
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T126 | 95-102 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T127 | 129-134 | Body_part | denotes | actin | http://purl.org/sig/ont/fma/fma67843 |
| T128 | 183-191 | Body_part | denotes | proteins | http://purl.org/sig/ont/fma/fma67257 |
| T129 | 250-255 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T130 | 281-288 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T131 | 476-480 | Body_part | denotes | cell | http://purl.org/sig/ont/fma/fma68646 |
| T132 | 563-567 | Body_part | denotes | milk | http://purl.org/sig/ont/fma/fma62100 |
| T133 | 667-672 | Body_part | denotes | actin | http://purl.org/sig/ont/fma/fma67843 |
| T134 | 879-886 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
LitCovid-PD-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T21 | 563-567 | Body_part | denotes | milk | http://purl.obolibrary.org/obo/UBERON_0001913 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T242 | 250-255 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T243 | 395-404 | http://purl.obolibrary.org/obo/UBERON_0000158 | denotes | membranes |
| T244 | 441-442 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T245 | 476-480 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
| T246 | 515-524 | http://purl.obolibrary.org/obo/UBERON_0000158 | denotes | membranes |
| T247 | 547-550 | http://purl.obolibrary.org/obo/CLO_0050644 | denotes | w/v |
| T248 | 581-585 | http://purl.obolibrary.org/obo/CLO_0001387 | denotes | 4 °C |
| T249 | 757-766 | http://purl.obolibrary.org/obo/UBERON_0000158 | denotes | membranes |
| T250 | 900-909 | http://purl.obolibrary.org/obo/UBERON_0000158 | denotes | membranes |
| T251 | 1030-1031 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
LitCovid-PD-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T134 | 95-102 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T135 | 183-191 | Chemical | denotes | proteins | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T136 | 281-288 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T137 | 319-322 | Chemical | denotes | SDS | http://purl.obolibrary.org/obo/CHEBI_8984 |
| T138 | 362-387 | Chemical | denotes | poly(vinylidene fluoride) | http://purl.obolibrary.org/obo/CHEBI_53250 |
| T139 | 367-377 | Chemical | denotes | vinylidene | http://purl.obolibrary.org/obo/CHEBI_29854 |
| T140 | 378-386 | Chemical | denotes | fluoride | http://purl.obolibrary.org/obo/CHEBI_17051 |
| T141 | 426-428 | Chemical | denotes | MA | http://purl.obolibrary.org/obo/CHEBI_474859|http://purl.obolibrary.org/obo/CHEBI_73610|http://purl.obolibrary.org/obo/CHEBI_90325 |
| T144 | 879-886 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T108 | 0-158 | Sentence | denotes | To further validate the identified DEPs, Western blot was performed to detect ANAPC7 and IFIT1 protein expression levels using β-actin as an internal control. |
| T109 | 159-266 | Sentence | denotes | Briefly, total cellular proteins were extracted from both mock- and PDCoV-infected IPEC-J2 cells at 24 hpi. |
| T110 | 267-510 | Sentence | denotes | The resulting protein samples were separated on 12% SDS-PAGE gels and transferred onto 0.22 μm poly(vinylidene fluoride) (PVDF) membranes (Millipore, Bedford, MA, USA) using a semidry electrophoretic transfer cell (Bio-Rad, Hercules, CA, USA). |
| T111 | 511-718 | Sentence | denotes | The membranes were blocked with 5% (w/v) nonfat dry milk overnight at 4 °C and then probed with either anti-ANAPC7 (1:1000), anti-IFIT1 (1:1000), or anti-β-actin (1:5000) primary antibodies at 37 °C for 1 h. |
| T112 | 719-867 | Sentence | denotes | After thorough washing with PBST, the membranes were incubated with the corresponding HRP-conjugated secondary antibodies (1:8000) for 1 h at 37 °C. |
| T113 | 868-1094 | Sentence | denotes | The target protein blots on the membranes were developed with an enhanced chemiluminescence detection kit (Thermo Fisher Scientific), and images were taken using a ProteinSimple FluorChem E image system (Santa Clara, CA, USA). |
LitCovid-PubTator
| Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
|---|---|---|---|---|---|
| 326 | 78-84 | Gene | denotes | ANAPC7 | Gene:51434 |
| 327 | 89-94 | Gene | denotes | IFIT1 | Gene:3434 |
| 328 | 127-134 | Gene | denotes | β-actin | Gene:728378 |
| 329 | 486-489 | Gene | denotes | Rad | Gene:6236 |
| 330 | 619-625 | Gene | denotes | ANAPC7 | Gene:51434 |
| 331 | 641-646 | Gene | denotes | IFIT1 | Gene:3434 |
| 332 | 970-973 | Gene | denotes | kit | Gene:3815 |
| 333 | 665-672 | Gene | denotes | β-actin | Gene:728378 |
| 334 | 319-322 | Chemical | denotes | SDS | MESH:D012967 |
| 335 | 362-387 | Chemical | denotes | poly(vinylidene fluoride) | MESH:C024865 |
| 336 | 389-393 | Chemical | denotes | PVDF | MESH:C024865 |
| 337 | 227-241 | Disease | denotes | PDCoV-infected | MESH:D007239 |
| 338 | 559-567 | Disease | denotes | dry milk | MESH:D016269 |
| 339 | 242-249 | CellLine | denotes | IPEC-J2 | CVCL:2246 |