PMC:7640975 / 12890-14225 JSONTXT

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The mass spectrometer was run using a data-dependent Top-20 acquisition mode, switching automatically between MS and MS/MS. A full MS scan ranging from 350 to 1300 m/z was conducted at 70 000 resolution with an automatic gain control (AGC) target value of 3 × 106 ions and a maximum ion transfer (IT) of 20 ms. The precursor ions were fragmented by means of high-energy collisional dissociation (HCD), and all MS/MS spectra were scanned using the following parameters: resolution 17 500; AGC 1 × 105 ions; maximum IT 50 ms; dynamic exclusion duration 18 s; normalized collision energy 30%; and intensity threshold 1.6 × 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