PMC:7574920 / 48001-49301 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T185","span":{"begin":624,"end":627},"obj":"Body_part"},{"id":"T186","span":{"begin":698,"end":701},"obj":"Body_part"},{"id":"T187","span":{"begin":886,"end":889},"obj":"Body_part"}],"attributes":[{"id":"A185","pred":"fma_id","subj":"T185","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A186","pred":"fma_id","subj":"T186","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A187","pred":"fma_id","subj":"T187","obj":"http://purl.org/sig/ont/fma/fma67095"}],"text":"Liquid handling using 96-well plates and precautions taken to prevent contamination\nTo prevent cross-contamination, we have taken several precautions. The 10× primer mix was prepared with nuclease-free water (AM9937, Ambion) and stored in aliquots at −20°C. To set up an RT-LAMP test, the RT-LAMP master mix was prepared freshly immediately before the test at a separate workspace with a dedicated pipette set and filter tips. The 96-well PCR plate containing the RT-LAMP mix was covered with an Society for Biomolecular Screening (SBS) plate lid. To avoid mix-ups during sample addition through well-by-well pipetting, the RNA or swab specimens were first collected into a 96-well seed plate. The RNA was then added to the plate with the LAMP reagents at a dedicated workspace with a manual 96-channel pipettor (Liquidator 20 μl, Mettler Toledo) using filter tips. The RT-LAMP and the RNA seed plate were instantly sealed with an optically clear adhesive seal (GK480-OS, Kisker Biotech) and an adhesive aluminum foil seal (SL-AM0550, Steinbrenner Laborsysteme, Germany), respectively. If the product of an RT-LAMP reaction had to be analyzed by gel electrophoresis, the plate was opened with extreme caution at a separated post-LAMP workspace and loaded onto an agarose gel with a dedicated pipette."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T103","span":{"begin":532,"end":535},"obj":"Disease"}],"attributes":[{"id":"A103","pred":"mondo_id","subj":"T103","obj":"http://purl.obolibrary.org/obo/MONDO_0011526"}],"text":"Liquid handling using 96-well plates and precautions taken to prevent contamination\nTo prevent cross-contamination, we have taken several precautions. The 10× primer mix was prepared with nuclease-free water (AM9937, Ambion) and stored in aliquots at −20°C. To set up an RT-LAMP test, the RT-LAMP master mix was prepared freshly immediately before the test at a separate workspace with a dedicated pipette set and filter tips. The 96-well PCR plate containing the RT-LAMP mix was covered with an Society for Biomolecular Screening (SBS) plate lid. To avoid mix-ups during sample addition through well-by-well pipetting, the RNA or swab specimens were first collected into a 96-well seed plate. The RNA was then added to the plate with the LAMP reagents at a dedicated workspace with a manual 96-channel pipettor (Liquidator 20 μl, Mettler Toledo) using filter tips. The RT-LAMP and the RNA seed plate were instantly sealed with an optically clear adhesive seal (GK480-OS, Kisker Biotech) and an adhesive aluminum foil seal (SL-AM0550, Steinbrenner Laborsysteme, Germany), respectively. If the product of an RT-LAMP reaction had to be analyzed by gel electrophoresis, the plate was opened with extreme caution at a separated post-LAMP workspace and loaded onto an agarose gel with a dedicated pipette."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T404","span":{"begin":279,"end":283},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T405","span":{"begin":352,"end":356},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T406","span":{"begin":360,"end":361},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T407","span":{"begin":386,"end":387},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T408","span":{"begin":672,"end":673},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T409","span":{"begin":756,"end":757},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T410","span":{"begin":783,"end":784},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T411","span":{"begin":839,"end":845},"obj":"http://purl.obolibrary.org/obo/CLO_0009382"},{"id":"T412","span":{"begin":1193,"end":1200},"obj":"http://www.ebi.ac.uk/efo/EFO_0000876"},{"id":"T413","span":{"begin":1212,"end":1213},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T414","span":{"begin":1280,"end":1281},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"Liquid handling using 96-well plates and precautions taken to prevent contamination\nTo prevent cross-contamination, we have taken several precautions. The 10× primer mix was prepared with nuclease-free water (AM9937, Ambion) and stored in aliquots at −20°C. To set up an RT-LAMP test, the RT-LAMP master mix was prepared freshly immediately before the test at a separate workspace with a dedicated pipette set and filter tips. The 96-well PCR plate containing the RT-LAMP mix was covered with an Society for Biomolecular Screening (SBS) plate lid. To avoid mix-ups during sample addition through well-by-well pipetting, the RNA or swab specimens were first collected into a 96-well seed plate. The RNA was then added to the plate with the LAMP reagents at a dedicated workspace with a manual 96-channel pipettor (Liquidator 20 μl, Mettler Toledo) using filter tips. The RT-LAMP and the RNA seed plate were instantly sealed with an optically clear adhesive seal (GK480-OS, Kisker Biotech) and an adhesive aluminum foil seal (SL-AM0550, Steinbrenner Laborsysteme, Germany), respectively. If the product of an RT-LAMP reaction had to be analyzed by gel electrophoresis, the plate was opened with extreme caution at a separated post-LAMP workspace and loaded onto an agarose gel with a dedicated pipette."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T98","span":{"begin":202,"end":207},"obj":"Chemical"},{"id":"T99","span":{"begin":744,"end":752},"obj":"Chemical"},{"id":"T100","span":{"begin":1004,"end":1012},"obj":"Chemical"},{"id":"T101","span":{"begin":1024,"end":1026},"obj":"Chemical"},{"id":"T102","span":{"begin":1263,"end":1270},"obj":"Chemical"}],"attributes":[{"id":"A98","pred":"chebi_id","subj":"T98","obj":"http://purl.obolibrary.org/obo/CHEBI_15377"},{"id":"A99","pred":"chebi_id","subj":"T99","obj":"http://purl.obolibrary.org/obo/CHEBI_33893"},{"id":"A100","pred":"chebi_id","subj":"T100","obj":"http://purl.obolibrary.org/obo/CHEBI_28984"},{"id":"A101","pred":"chebi_id","subj":"T101","obj":"http://purl.obolibrary.org/obo/CHEBI_74815"},{"id":"A102","pred":"chebi_id","subj":"T102","obj":"http://purl.obolibrary.org/obo/CHEBI_2511"}],"text":"Liquid handling using 96-well plates and precautions taken to prevent contamination\nTo prevent cross-contamination, we have taken several precautions. The 10× primer mix was prepared with nuclease-free water (AM9937, Ambion) and stored in aliquots at −20°C. To set up an RT-LAMP test, the RT-LAMP master mix was prepared freshly immediately before the test at a separate workspace with a dedicated pipette set and filter tips. The 96-well PCR plate containing the RT-LAMP mix was covered with an Society for Biomolecular Screening (SBS) plate lid. To avoid mix-ups during sample addition through well-by-well pipetting, the RNA or swab specimens were first collected into a 96-well seed plate. The RNA was then added to the plate with the LAMP reagents at a dedicated workspace with a manual 96-channel pipettor (Liquidator 20 μl, Mettler Toledo) using filter tips. The RT-LAMP and the RNA seed plate were instantly sealed with an optically clear adhesive seal (GK480-OS, Kisker Biotech) and an adhesive aluminum foil seal (SL-AM0550, Steinbrenner Laborsysteme, Germany), respectively. If the product of an RT-LAMP reaction had to be analyzed by gel electrophoresis, the plate was opened with extreme caution at a separated post-LAMP workspace and loaded onto an agarose gel with a dedicated pipette."}

{"project":"LitCovid-PubTator","denotations":[{"id":"323","span":{"begin":557,"end":560},"obj":"Gene"},{"id":"324","span":{"begin":472,"end":475},"obj":"Gene"},{"id":"325","span":{"begin":304,"end":307},"obj":"Gene"},{"id":"326","span":{"begin":166,"end":169},"obj":"Gene"},{"id":"327","span":{"begin":202,"end":207},"obj":"Chemical"},{"id":"328","span":{"begin":1004,"end":1012},"obj":"Chemical"},{"id":"329","span":{"begin":1263,"end":1270},"obj":"Chemical"}],"attributes":[{"id":"A323","pred":"tao:has_database_id","subj":"323","obj":"Gene:83881"},{"id":"A324","pred":"tao:has_database_id","subj":"324","obj":"Gene:83881"},{"id":"A325","pred":"tao:has_database_id","subj":"325","obj":"Gene:83881"},{"id":"A326","pred":"tao:has_database_id","subj":"326","obj":"Gene:83881"},{"id":"A327","pred":"tao:has_database_id","subj":"327","obj":"MESH:D014867"},{"id":"A328","pred":"tao:has_database_id","subj":"328","obj":"MESH:D000535"},{"id":"A329","pred":"tao:has_database_id","subj":"329","obj":"MESH:D012685"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Liquid handling using 96-well plates and precautions taken to prevent contamination\nTo prevent cross-contamination, we have taken several precautions. The 10× primer mix was prepared with nuclease-free water (AM9937, Ambion) and stored in aliquots at −20°C. To set up an RT-LAMP test, the RT-LAMP master mix was prepared freshly immediately before the test at a separate workspace with a dedicated pipette set and filter tips. The 96-well PCR plate containing the RT-LAMP mix was covered with an Society for Biomolecular Screening (SBS) plate lid. To avoid mix-ups during sample addition through well-by-well pipetting, the RNA or swab specimens were first collected into a 96-well seed plate. The RNA was then added to the plate with the LAMP reagents at a dedicated workspace with a manual 96-channel pipettor (Liquidator 20 μl, Mettler Toledo) using filter tips. The RT-LAMP and the RNA seed plate were instantly sealed with an optically clear adhesive seal (GK480-OS, Kisker Biotech) and an adhesive aluminum foil seal (SL-AM0550, Steinbrenner Laborsysteme, Germany), respectively. If the product of an RT-LAMP reaction had to be analyzed by gel electrophoresis, the plate was opened with extreme caution at a separated post-LAMP workspace and loaded onto an agarose gel with a dedicated pipette."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T281","span":{"begin":271,"end":273},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T282","span":{"begin":289,"end":291},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T283","span":{"begin":464,"end":466},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T284","span":{"begin":870,"end":872},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T285","span":{"begin":1107,"end":1109},"obj":"http://purl.obolibrary.org/obo/GO_0001171"}],"text":"Liquid handling using 96-well plates and precautions taken to prevent contamination\nTo prevent cross-contamination, we have taken several precautions. The 10× primer mix was prepared with nuclease-free water (AM9937, Ambion) and stored in aliquots at −20°C. To set up an RT-LAMP test, the RT-LAMP master mix was prepared freshly immediately before the test at a separate workspace with a dedicated pipette set and filter tips. The 96-well PCR plate containing the RT-LAMP mix was covered with an Society for Biomolecular Screening (SBS) plate lid. To avoid mix-ups during sample addition through well-by-well pipetting, the RNA or swab specimens were first collected into a 96-well seed plate. The RNA was then added to the plate with the LAMP reagents at a dedicated workspace with a manual 96-channel pipettor (Liquidator 20 μl, Mettler Toledo) using filter tips. The RT-LAMP and the RNA seed plate were instantly sealed with an optically clear adhesive seal (GK480-OS, Kisker Biotech) and an adhesive aluminum foil seal (SL-AM0550, Steinbrenner Laborsysteme, Germany), respectively. If the product of an RT-LAMP reaction had to be analyzed by gel electrophoresis, the plate was opened with extreme caution at a separated post-LAMP workspace and loaded onto an agarose gel with a dedicated pipette."}

{"project":"LitCovid-sentences","denotations":[{"id":"T347","span":{"begin":0,"end":83},"obj":"Sentence"},{"id":"T348","span":{"begin":84,"end":150},"obj":"Sentence"},{"id":"T349","span":{"begin":151,"end":257},"obj":"Sentence"},{"id":"T350","span":{"begin":258,"end":426},"obj":"Sentence"},{"id":"T351","span":{"begin":427,"end":547},"obj":"Sentence"},{"id":"T352","span":{"begin":548,"end":693},"obj":"Sentence"},{"id":"T353","span":{"begin":694,"end":865},"obj":"Sentence"},{"id":"T354","span":{"begin":866,"end":1085},"obj":"Sentence"},{"id":"T355","span":{"begin":1086,"end":1300},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Liquid handling using 96-well plates and precautions taken to prevent contamination\nTo prevent cross-contamination, we have taken several precautions. The 10× primer mix was prepared with nuclease-free water (AM9937, Ambion) and stored in aliquots at −20°C. To set up an RT-LAMP test, the RT-LAMP master mix was prepared freshly immediately before the test at a separate workspace with a dedicated pipette set and filter tips. The 96-well PCR plate containing the RT-LAMP mix was covered with an Society for Biomolecular Screening (SBS) plate lid. To avoid mix-ups during sample addition through well-by-well pipetting, the RNA or swab specimens were first collected into a 96-well seed plate. The RNA was then added to the plate with the LAMP reagents at a dedicated workspace with a manual 96-channel pipettor (Liquidator 20 μl, Mettler Toledo) using filter tips. The RT-LAMP and the RNA seed plate were instantly sealed with an optically clear adhesive seal (GK480-OS, Kisker Biotech) and an adhesive aluminum foil seal (SL-AM0550, Steinbrenner Laborsysteme, Germany), respectively. If the product of an RT-LAMP reaction had to be analyzed by gel electrophoresis, the plate was opened with extreme caution at a separated post-LAMP workspace and loaded onto an agarose gel with a dedicated pipette."}