PMC:7574920 / 48001-49301
Annnotations
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T185 | 624-627 | Body_part | denotes | RNA | http://purl.org/sig/ont/fma/fma67095 |
| T186 | 698-701 | Body_part | denotes | RNA | http://purl.org/sig/ont/fma/fma67095 |
| T187 | 886-889 | Body_part | denotes | RNA | http://purl.org/sig/ont/fma/fma67095 |
LitCovid-PD-MONDO
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T103 | 532-535 | Disease | denotes | SBS | http://purl.obolibrary.org/obo/MONDO_0011526 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T404 | 279-283 | http://purl.obolibrary.org/obo/UBERON_0000473 | denotes | test |
| T405 | 352-356 | http://purl.obolibrary.org/obo/UBERON_0000473 | denotes | test |
| T406 | 360-361 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T407 | 386-387 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T408 | 672-673 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T409 | 756-757 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T410 | 783-784 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T411 | 839-845 | http://purl.obolibrary.org/obo/CLO_0009382 | denotes | Toledo |
| T412 | 1193-1200 | http://www.ebi.ac.uk/efo/EFO_0000876 | denotes | extreme |
| T413 | 1212-1213 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T414 | 1280-1281 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
LitCovid-PD-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T98 | 202-207 | Chemical | denotes | water | http://purl.obolibrary.org/obo/CHEBI_15377 |
| T99 | 744-752 | Chemical | denotes | reagents | http://purl.obolibrary.org/obo/CHEBI_33893 |
| T100 | 1004-1012 | Chemical | denotes | aluminum | http://purl.obolibrary.org/obo/CHEBI_28984 |
| T101 | 1024-1026 | Chemical | denotes | SL | http://purl.obolibrary.org/obo/CHEBI_74815 |
| T102 | 1263-1270 | Chemical | denotes | agarose | http://purl.obolibrary.org/obo/CHEBI_2511 |
LitCovid-PubTator
| Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
|---|---|---|---|---|---|
| 323 | 557-560 | Gene | denotes | mix | Gene:83881 |
| 324 | 472-475 | Gene | denotes | mix | Gene:83881 |
| 325 | 304-307 | Gene | denotes | mix | Gene:83881 |
| 326 | 166-169 | Gene | denotes | mix | Gene:83881 |
| 327 | 202-207 | Chemical | denotes | water | MESH:D014867 |
| 328 | 1004-1012 | Chemical | denotes | aluminum | MESH:D000535 |
| 329 | 1263-1270 | Chemical | denotes | agarose | MESH:D012685 |
LitCovid-PD-GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T281 | 271-273 | http://purl.obolibrary.org/obo/GO_0001171 | denotes | RT |
| T282 | 289-291 | http://purl.obolibrary.org/obo/GO_0001171 | denotes | RT |
| T283 | 464-466 | http://purl.obolibrary.org/obo/GO_0001171 | denotes | RT |
| T284 | 870-872 | http://purl.obolibrary.org/obo/GO_0001171 | denotes | RT |
| T285 | 1107-1109 | http://purl.obolibrary.org/obo/GO_0001171 | denotes | RT |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T347 | 0-83 | Sentence | denotes | Liquid handling using 96-well plates and precautions taken to prevent contamination |
| T348 | 84-150 | Sentence | denotes | To prevent cross-contamination, we have taken several precautions. |
| T349 | 151-257 | Sentence | denotes | The 10× primer mix was prepared with nuclease-free water (AM9937, Ambion) and stored in aliquots at −20°C. |
| T350 | 258-426 | Sentence | denotes | To set up an RT-LAMP test, the RT-LAMP master mix was prepared freshly immediately before the test at a separate workspace with a dedicated pipette set and filter tips. |
| T351 | 427-547 | Sentence | denotes | The 96-well PCR plate containing the RT-LAMP mix was covered with an Society for Biomolecular Screening (SBS) plate lid. |
| T352 | 548-693 | Sentence | denotes | To avoid mix-ups during sample addition through well-by-well pipetting, the RNA or swab specimens were first collected into a 96-well seed plate. |
| T353 | 694-865 | Sentence | denotes | The RNA was then added to the plate with the LAMP reagents at a dedicated workspace with a manual 96-channel pipettor (Liquidator 20 μl, Mettler Toledo) using filter tips. |
| T354 | 866-1085 | Sentence | denotes | The RT-LAMP and the RNA seed plate were instantly sealed with an optically clear adhesive seal (GK480-OS, Kisker Biotech) and an adhesive aluminum foil seal (SL-AM0550, Steinbrenner Laborsysteme, Germany), respectively. |
| T355 | 1086-1300 | Sentence | denotes | If the product of an RT-LAMP reaction had to be analyzed by gel electrophoresis, the plate was opened with extreme caution at a separated post-LAMP workspace and loaded onto an agarose gel with a dedicated pipette. |