PMC:7574920 / 47231-47999 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T181","span":{"begin":146,"end":150},"obj":"Body_part"},{"id":"T182","span":{"begin":398,"end":401},"obj":"Body_part"},{"id":"T183","span":{"begin":429,"end":433},"obj":"Body_part"},{"id":"T184","span":{"begin":524,"end":527},"obj":"Body_part"}],"attributes":[{"id":"A181","pred":"fma_id","subj":"T181","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A182","pred":"fma_id","subj":"T182","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A183","pred":"fma_id","subj":"T183","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A184","pred":"fma_id","subj":"T184","obj":"http://purl.org/sig/ont/fma/fma74412"}],"text":"RT-LAMP primer design and positive control\nThe RT-LAMP primer sets used in this study have been designed by Zhang et al. (11) against ORF1a and N gene and were synthesized by Sigma-Aldrich (synthesis scale, 0.025 μmol; purification, desalt; solution, water). The sequences and the concentrations of each oligonucleotide in the 10× primer mix used for the RT-LAMP assay can be found in table S1.\nAn RNA-positive control for the N gene was amplified from a short fragment from 2019-nCoV_N_Positive control plasmid [Integrated DNA Technologies (IDT), 10006625] with oligonucleotides T7-GeneN-Fragment.for and GeneN-Fragment.rev including the T7 promoter and a subsequent IVT with the MEGAscript T7 Kit (Invitrogen) purified using the RNeasy MinElute Cleanup Kit (QIAGEN)."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T15","span":{"begin":200,"end":205},"obj":"Body_part"}],"attributes":[{"id":"A15","pred":"uberon_id","subj":"T15","obj":"http://purl.obolibrary.org/obo/UBERON_0002542"}],"text":"RT-LAMP primer design and positive control\nThe RT-LAMP primer sets used in this study have been designed by Zhang et al. (11) against ORF1a and N gene and were synthesized by Sigma-Aldrich (synthesis scale, 0.025 μmol; purification, desalt; solution, water). The sequences and the concentrations of each oligonucleotide in the 10× primer mix used for the RT-LAMP assay can be found in table S1.\nAn RNA-positive control for the N gene was amplified from a short fragment from 2019-nCoV_N_Positive control plasmid [Integrated DNA Technologies (IDT), 10006625] with oligonucleotides T7-GeneN-Fragment.for and GeneN-Fragment.rev including the T7 promoter and a subsequent IVT with the MEGAscript T7 Kit (Invitrogen) purified using the RNeasy MinElute Cleanup Kit (QIAGEN)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T398","span":{"begin":122,"end":124},"obj":"http://purl.obolibrary.org/obo/CLO_0053733"},{"id":"T399","span":{"begin":146,"end":150},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T400","span":{"begin":391,"end":393},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T401","span":{"begin":429,"end":433},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T402","span":{"begin":453,"end":454},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T403","span":{"begin":655,"end":656},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"RT-LAMP primer design and positive control\nThe RT-LAMP primer sets used in this study have been designed by Zhang et al. (11) against ORF1a and N gene and were synthesized by Sigma-Aldrich (synthesis scale, 0.025 μmol; purification, desalt; solution, water). The sequences and the concentrations of each oligonucleotide in the 10× primer mix used for the RT-LAMP assay can be found in table S1.\nAn RNA-positive control for the N gene was amplified from a short fragment from 2019-nCoV_N_Positive control plasmid [Integrated DNA Technologies (IDT), 10006625] with oligonucleotides T7-GeneN-Fragment.for and GeneN-Fragment.rev including the T7 promoter and a subsequent IVT with the MEGAscript T7 Kit (Invitrogen) purified using the RNeasy MinElute Cleanup Kit (QIAGEN)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T93","span":{"begin":241,"end":249},"obj":"Chemical"},{"id":"T94","span":{"begin":251,"end":256},"obj":"Chemical"},{"id":"T95","span":{"begin":304,"end":319},"obj":"Chemical"},{"id":"T96","span":{"begin":524,"end":527},"obj":"Chemical"},{"id":"T97","span":{"begin":563,"end":579},"obj":"Chemical"}],"attributes":[{"id":"A93","pred":"chebi_id","subj":"T93","obj":"http://purl.obolibrary.org/obo/CHEBI_75958"},{"id":"A94","pred":"chebi_id","subj":"T94","obj":"http://purl.obolibrary.org/obo/CHEBI_15377"},{"id":"A95","pred":"chebi_id","subj":"T95","obj":"http://purl.obolibrary.org/obo/CHEBI_7754"},{"id":"A96","pred":"chebi_id","subj":"T96","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"},{"id":"A97","pred":"chebi_id","subj":"T97","obj":"http://purl.obolibrary.org/obo/CHEBI_7754"}],"text":"RT-LAMP primer design and positive control\nThe RT-LAMP primer sets used in this study have been designed by Zhang et al. (11) against ORF1a and N gene and were synthesized by Sigma-Aldrich (synthesis scale, 0.025 μmol; purification, desalt; solution, water). The sequences and the concentrations of each oligonucleotide in the 10× primer mix used for the RT-LAMP assay can be found in table S1.\nAn RNA-positive control for the N gene was amplified from a short fragment from 2019-nCoV_N_Positive control plasmid [Integrated DNA Technologies (IDT), 10006625] with oligonucleotides T7-GeneN-Fragment.for and GeneN-Fragment.rev including the T7 promoter and a subsequent IVT with the MEGAscript T7 Kit (Invitrogen) purified using the RNeasy MinElute Cleanup Kit (QIAGEN)."}

{"project":"LitCovid-PubTator","denotations":[{"id":"307","span":{"begin":134,"end":139},"obj":"Gene"},{"id":"308","span":{"begin":144,"end":145},"obj":"Gene"},{"id":"309","span":{"begin":338,"end":341},"obj":"Gene"},{"id":"310","span":{"begin":251,"end":256},"obj":"Chemical"},{"id":"311","span":{"begin":304,"end":319},"obj":"Chemical"},{"id":"314","span":{"begin":427,"end":428},"obj":"Gene"},{"id":"315","span":{"begin":485,"end":486},"obj":"Gene"}],"attributes":[{"id":"A307","pred":"tao:has_database_id","subj":"307","obj":"Gene:43740578"},{"id":"A308","pred":"tao:has_database_id","subj":"308","obj":"Gene:43740575"},{"id":"A309","pred":"tao:has_database_id","subj":"309","obj":"Gene:83881"},{"id":"A310","pred":"tao:has_database_id","subj":"310","obj":"MESH:D014867"},{"id":"A311","pred":"tao:has_database_id","subj":"311","obj":"MESH:D009841"},{"id":"A314","pred":"tao:has_database_id","subj":"314","obj":"Gene:43740575"},{"id":"A315","pred":"tao:has_database_id","subj":"315","obj":"Gene:43740575"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"RT-LAMP primer design and positive control\nThe RT-LAMP primer sets used in this study have been designed by Zhang et al. (11) against ORF1a and N gene and were synthesized by Sigma-Aldrich (synthesis scale, 0.025 μmol; purification, desalt; solution, water). The sequences and the concentrations of each oligonucleotide in the 10× primer mix used for the RT-LAMP assay can be found in table S1.\nAn RNA-positive control for the N gene was amplified from a short fragment from 2019-nCoV_N_Positive control plasmid [Integrated DNA Technologies (IDT), 10006625] with oligonucleotides T7-GeneN-Fragment.for and GeneN-Fragment.rev including the T7 promoter and a subsequent IVT with the MEGAscript T7 Kit (Invitrogen) purified using the RNeasy MinElute Cleanup Kit (QIAGEN)."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T277","span":{"begin":0,"end":2},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T278","span":{"begin":47,"end":49},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T279","span":{"begin":190,"end":199},"obj":"http://purl.obolibrary.org/obo/GO_0009058"},{"id":"T280","span":{"begin":355,"end":357},"obj":"http://purl.obolibrary.org/obo/GO_0001171"}],"text":"RT-LAMP primer design and positive control\nThe RT-LAMP primer sets used in this study have been designed by Zhang et al. (11) against ORF1a and N gene and were synthesized by Sigma-Aldrich (synthesis scale, 0.025 μmol; purification, desalt; solution, water). The sequences and the concentrations of each oligonucleotide in the 10× primer mix used for the RT-LAMP assay can be found in table S1.\nAn RNA-positive control for the N gene was amplified from a short fragment from 2019-nCoV_N_Positive control plasmid [Integrated DNA Technologies (IDT), 10006625] with oligonucleotides T7-GeneN-Fragment.for and GeneN-Fragment.rev including the T7 promoter and a subsequent IVT with the MEGAscript T7 Kit (Invitrogen) purified using the RNeasy MinElute Cleanup Kit (QIAGEN)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T343","span":{"begin":0,"end":42},"obj":"Sentence"},{"id":"T344","span":{"begin":43,"end":258},"obj":"Sentence"},{"id":"T345","span":{"begin":259,"end":394},"obj":"Sentence"},{"id":"T346","span":{"begin":395,"end":768},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"RT-LAMP primer design and positive control\nThe RT-LAMP primer sets used in this study have been designed by Zhang et al. (11) against ORF1a and N gene and were synthesized by Sigma-Aldrich (synthesis scale, 0.025 μmol; purification, desalt; solution, water). The sequences and the concentrations of each oligonucleotide in the 10× primer mix used for the RT-LAMP assay can be found in table S1.\nAn RNA-positive control for the N gene was amplified from a short fragment from 2019-nCoV_N_Positive control plasmid [Integrated DNA Technologies (IDT), 10006625] with oligonucleotides T7-GeneN-Fragment.for and GeneN-Fragment.rev including the T7 promoter and a subsequent IVT with the MEGAscript T7 Kit (Invitrogen) purified using the RNeasy MinElute Cleanup Kit (QIAGEN)."}