PMC:7574920 / 41680-42196 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T152","span":{"begin":276,"end":279},"obj":"Body_part"}],"attributes":[{"id":"A152","pred":"fma_id","subj":"T152","obj":"http://purl.org/sig/ont/fma/fma67095"}],"text":"We used LAMP-sequencing to validate the RT-LAMP assay results and did not use it as a diagnostic tool. LAMP-sequencing is dependent on the sensitivity of the RT-LAMP reaction as it cannot detect false negative results caused by a failure of the RT-LAMP assay to amplify viral RNA. Also, reagents such as the primer sets for the RT-LAMP assay may be subject to production-dependent quality fluctuations. Therefore, all reagents must be precisely validated (batch control) before using an RT-LAMP assay diagnostically."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T352","span":{"begin":84,"end":85},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T353","span":{"begin":228,"end":229},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"We used LAMP-sequencing to validate the RT-LAMP assay results and did not use it as a diagnostic tool. LAMP-sequencing is dependent on the sensitivity of the RT-LAMP reaction as it cannot detect false negative results caused by a failure of the RT-LAMP assay to amplify viral RNA. Also, reagents such as the primer sets for the RT-LAMP assay may be subject to production-dependent quality fluctuations. Therefore, all reagents must be precisely validated (batch control) before using an RT-LAMP assay diagnostically."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T83","span":{"begin":287,"end":295},"obj":"Chemical"},{"id":"T84","span":{"begin":418,"end":426},"obj":"Chemical"}],"attributes":[{"id":"A83","pred":"chebi_id","subj":"T83","obj":"http://purl.obolibrary.org/obo/CHEBI_33893"},{"id":"A84","pred":"chebi_id","subj":"T84","obj":"http://purl.obolibrary.org/obo/CHEBI_33893"}],"text":"We used LAMP-sequencing to validate the RT-LAMP assay results and did not use it as a diagnostic tool. LAMP-sequencing is dependent on the sensitivity of the RT-LAMP reaction as it cannot detect false negative results caused by a failure of the RT-LAMP assay to amplify viral RNA. Also, reagents such as the primer sets for the RT-LAMP assay may be subject to production-dependent quality fluctuations. Therefore, all reagents must be precisely validated (batch control) before using an RT-LAMP assay diagnostically."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T249","span":{"begin":40,"end":42},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T250","span":{"begin":158,"end":160},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T251","span":{"begin":245,"end":247},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T252","span":{"begin":328,"end":330},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T253","span":{"begin":487,"end":489},"obj":"http://purl.obolibrary.org/obo/GO_0001171"}],"text":"We used LAMP-sequencing to validate the RT-LAMP assay results and did not use it as a diagnostic tool. LAMP-sequencing is dependent on the sensitivity of the RT-LAMP reaction as it cannot detect false negative results caused by a failure of the RT-LAMP assay to amplify viral RNA. Also, reagents such as the primer sets for the RT-LAMP assay may be subject to production-dependent quality fluctuations. Therefore, all reagents must be precisely validated (batch control) before using an RT-LAMP assay diagnostically."}

{"project":"LitCovid-sentences","denotations":[{"id":"T306","span":{"begin":0,"end":102},"obj":"Sentence"},{"id":"T307","span":{"begin":103,"end":280},"obj":"Sentence"},{"id":"T308","span":{"begin":281,"end":402},"obj":"Sentence"},{"id":"T309","span":{"begin":403,"end":516},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"We used LAMP-sequencing to validate the RT-LAMP assay results and did not use it as a diagnostic tool. LAMP-sequencing is dependent on the sensitivity of the RT-LAMP reaction as it cannot detect false negative results caused by a failure of the RT-LAMP assay to amplify viral RNA. Also, reagents such as the primer sets for the RT-LAMP assay may be subject to production-dependent quality fluctuations. Therefore, all reagents must be precisely validated (batch control) before using an RT-LAMP assay diagnostically."}