PMC:7574920 / 36311-37004
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7574920","sourcedb":"PMC","sourceid":"7574920","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7574920","text":"Although spike-in experiments with IVT RNA can be informative, we have experienced clear differences when comparing such experiments to those using clinical RNA samples isolated from swab specimens (figs. S6 and S7, and data file S1). We therefore recommend validating any new proposed rapid SARS-CoV-2 diagnostic test using “real-life” clinical samples including a large fraction of negative clinical samples. To overcome the problem of spurious amplification, an expanded oligonucleotide set that incorporates sequence-specific probes (34) or a CRISPR/Cas12a–based approach (36) could be used. However, these applications have yet to be tested with large numbers of diverse clinical samples.","tracks":[{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T143","span":{"begin":39,"end":42},"obj":"Body_part"},{"id":"T144","span":{"begin":157,"end":160},"obj":"Body_part"}],"attributes":[{"id":"A143","pred":"fma_id","subj":"T143","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A144","pred":"fma_id","subj":"T144","obj":"http://purl.org/sig/ont/fma/fma67095"},{"subj":"T143","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T144","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T83","span":{"begin":292,"end":300},"obj":"Disease"}],"attributes":[{"id":"A83","pred":"mondo_id","subj":"T83","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"subj":"T83","pred":"source","obj":"LitCovid-PD-MONDO"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T310","span":{"begin":230,"end":232},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T311","span":{"begin":314,"end":318},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T312","span":{"begin":364,"end":365},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T313","span":{"begin":538,"end":540},"obj":"http://purl.obolibrary.org/obo/CLO_0001302"},{"id":"T314","span":{"begin":545,"end":546},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T315","span":{"begin":577,"end":579},"obj":"http://purl.obolibrary.org/obo/CLO_0001313"},{"id":"T316","span":{"begin":639,"end":645},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"}],"attributes":[{"subj":"T310","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T311","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T312","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T313","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T314","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T315","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T316","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T71","span":{"begin":474,"end":489},"obj":"Chemical"}],"attributes":[{"id":"A71","pred":"chebi_id","subj":"T71","obj":"http://purl.obolibrary.org/obo/CHEBI_7754"},{"subj":"T71","pred":"source","obj":"LitCovid-PD-CHEBI"}]},{"project":"LitCovid-PubTator","denotations":[{"id":"252","span":{"begin":292,"end":302},"obj":"Species"},{"id":"253","span":{"begin":474,"end":489},"obj":"Chemical"}],"attributes":[{"id":"A252","pred":"tao:has_database_id","subj":"252","obj":"Tax:2697049"},{"id":"A253","pred":"tao:has_database_id","subj":"253","obj":"MESH:D009841"},{"subj":"252","pred":"source","obj":"LitCovid-PubTator"},{"subj":"253","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T271","span":{"begin":0,"end":204},"obj":"Sentence"},{"id":"T272","span":{"begin":205,"end":234},"obj":"Sentence"},{"id":"T273","span":{"begin":235,"end":410},"obj":"Sentence"},{"id":"T274","span":{"begin":411,"end":595},"obj":"Sentence"},{"id":"T275","span":{"begin":596,"end":693},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T271","pred":"source","obj":"LitCovid-sentences"},{"subj":"T272","pred":"source","obj":"LitCovid-sentences"},{"subj":"T273","pred":"source","obj":"LitCovid-sentences"},{"subj":"T274","pred":"source","obj":"LitCovid-sentences"},{"subj":"T275","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PD-FMA-UBERON","color":"#93ecd8","default":true},{"id":"LitCovid-PD-MONDO","color":"#ecbe93"},{"id":"LitCovid-PD-CLO","color":"#a493ec"},{"id":"LitCovid-PD-CHEBI","color":"#9bec93"},{"id":"LitCovid-PubTator","color":"#ec93b5"},{"id":"LitCovid-sentences","color":"#93cfec"}]}]}}