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PMC:7574920 / 26132-27232 JSONTXT

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LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T114 182-185 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T115 353-356 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T116 447-460 Body_part denotes RNA molecules http://purl.org/sig/ont/fma/fma84126
T117 447-450 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T118 481-485 Body_part denotes gene http://purl.org/sig/ont/fma/fma74402
T119 698-701 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T120 817-820 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T121 1054-1057 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T67 468-476 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T68 509-517 Disease denotes COVID-19 http://purl.obolibrary.org/obo/MONDO_0100096
T69 1043-1051 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T231 45-47 http://purl.obolibrary.org/obo/CLO_0050510 denotes 18
T232 76-78 http://purl.obolibrary.org/obo/CLO_0050507 denotes 22
T233 107-114 http://purl.obolibrary.org/obo/UBERON_0000473 denotes testing
T234 174-175 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T235 260-264 http://purl.obolibrary.org/obo/UBERON_0000473 denotes test
T236 481-485 http://purl.obolibrary.org/obo/OGG_0000000002 denotes gene
T237 548-554 http://purl.obolibrary.org/obo/UBERON_0000473 denotes tested
T238 636-641 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus
T239 728-733 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus
T240 767-769 http://purl.obolibrary.org/obo/CLO_0050050 denotes S1
T241 821-828 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes viruses
T242 841-846 http://purl.obolibrary.org/obo/UBERON_0000473 denotes tests

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T63 451-460 Chemical denotes molecules http://purl.obolibrary.org/obo/CHEBI_25367
T64 607-616 Chemical denotes detergent http://purl.obolibrary.org/obo/CHEBI_27780

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
217 479-480 Gene denotes N Gene:43740575
218 468-478 Species denotes SARS-CoV-2 Tax:2697049
219 1043-1053 Species denotes SARS-CoV-2 Tax:2697049
220 509-517 Disease denotes COVID-19 MESH:C000657245
221 1079-1087 Disease denotes infected MESH:D007239

LitCovid-PD-GO-BP

Id Subject Object Predicate Lexical cue
T153 36-38 http://purl.obolibrary.org/obo/GO_0001171 denotes RT
T154 56-58 http://purl.obolibrary.org/obo/GO_0001171 denotes RT
T155 235-237 http://purl.obolibrary.org/obo/GO_0001171 denotes RT
T156 296-298 http://purl.obolibrary.org/obo/GO_0001171 denotes RT
T157 900-902 http://purl.obolibrary.org/obo/GO_0001171 denotes RT

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T191 0-218 Sentence denotes Several reports have indicated that RT-qPCR (18–20) and RT-LAMP assays (21, 22) are compatible with direct testing of nasopharyngeal and oropharyngeal swab specimens without a prior RNA purification or extraction step.
T192 219-411 Sentence denotes To establish an RT-LAMP assay that could test unprocessed specimens (swab–to–RT-LAMP assay), we first assessed the stability of naked RNA in swab specimens that were collected in Amies medium.
T193 412-544 Sentence denotes We titrated defined numbers of IVT RNA molecules of the SARS-CoV-2 N gene into swab samples from COVID-19–negative control subjects.
T194 545-741 Sentence denotes We tested different conditions, particularly the influence of detergent (to inactivate the virus) and heat (to denature the capsid and release the viral RNA as well as inactivate the virus) (figs.
T195 742-771 Sentence denotes S6 and S7, and data file S1).
T196 772-1100 Sentence denotes Consistent with previous reports about other RNA viruses (23–25) and tests using heat inactivation of swab specimens for direct RT-qPCR assays (26), these experiments established that native swab specimens and heat-treated swab specimens were compatible for detection of SARS-CoV-2 RNA in swab samples from infected individuals.