PMC:7571312 / 89194-90525 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7571312","sourcedb":"PMC","sourceid":"7571312","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7571312","text":"N-((1S)-1-{[((1S)-3-Hydroxy-2-oxo-1-{[(3S)-2-oxopyrrolidin-3-yl]methyl}propyl)amino]carbonyl}pentyl)-4-methoxy-1H-indole-2-carboxamide (39)\nFollowing the procedure described for the preparation of N-((1S)-1-{[((1S)-3-hydroxy-2-oxo-1-{[(3S)-2-oxopyrrolidin-3-yl]methyl}propyl)amino]carbonyl}-3-methylbutyl)-4-methoxy-1H-indole-2-carboxamide but substituting of N-((1S)-1-{[((1S)-3-chloro-2-oxo-1-{[(3S)-2-oxopyrrolidin-3-yl]methyl}propyl)amino]carbonyl}pentyl)-4-methoxy-1H-indole-2-carboxamide and making noncritical variations provided a crude yellow foam. This material was purified by Biotage MPLC (25 M column, 5–6% methanol/chloroform) to afford 82 mg (35%) of the title compound as a white foam. 1H NMR (DMSO-d6) δ 11.57 (s, 1H), 8.42 (d, J = 8 Hz, 1H), 8.38 (d, J = 8 Hz, 1H), 7.62 (s, 1H), 7.35 (s, 1H), 7.09 (t, J = 8 Hz, 1H), 6.99 (d, J = 8 Hz, 1H), 6.49 (d, J = 8 Hz, 1H), 5.06 (t, J = 8 Hz, 1H), 4.44 (m, 2H), 4.25 (dd, J = 8, 20 Hz, 1H), 4.14 (dd, J = 8, 20 Hz, 1H), 3.87 (s, 3H), 3.08 (m, 2H), 2.28 (m, 1H), 2.10 (m, 1H), 1.91 (m, 1H), 1.74–1.54 (m, 4H), 1.32 (m, 4H), 0.87 (t, J = 8 Hz, 3H); MS (ESI+) for C24H32N4O6m/z 473.2 (M + H)+; anal. calcd for C24H32N4O6·0.6 H2O·0.2 ethyl acetate: C, 59.46; H, 7.01; N, 11.18. Found: C, 53.37; H, 6.94; N, 11.23; HRMS (ESI+) calcd for C24H32N4O6+H1 473.2395, found 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