> top > docs > PMC:7544934 > spans > 7403-8170 > annotations

PMC:7544934 / 7403-8170 JSONTXT

Annnotations TAB JSON ListView MergeView

LitCovid_Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T21 44-47 https://glytoucan.org/Structures/Glycans/G00057MO denotes LDN
T22 44-47 https://glytoucan.org/Structures/Glycans/G31736BK denotes LDN

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
208 133-144 Chemical denotes RIPA buffer
209 342-345 Chemical denotes SDS MESH:D012967
210 377-381 Chemical denotes PVDF MESH:C024865
211 537-541 Chemical denotes TBST
212 562-566 Chemical denotes TBST
213 0-8 CellLine denotes Raw264.7 CVCL:0493

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T60 0-95 Sentence denotes Raw264.7 cultured cells were incubated with LDN (0 and 5 µM) in the presence or absence of LPS.
T61 96-192 Sentence denotes After treatment, cells were lysed in RIPA buffer containing 1% protease- phosphatase inhibitors.
T62 193-319 Sentence denotes Protein concentration was determined by BCA assay reagent as described in the manufacturer’s (Thermo Scientific-23227) manual.
T63 320-392 Sentence denotes Protein was loaded on SDS-PAGE and electro-blotted on to PVDF membranes.
T64 393-682 Sentence denotes The membrane was incubated in 5% milk blocking solution for 2 h at room temperature (RT) and probed against primary antibody (1:2000 diluted in TBST; After washing with TBST, the membrane was incubated with HRP conjugated IgG secondary antibody for 2 h and visualized by chemiluminescence.
T65 683-767 Sentence denotes A list of antibodies is provided in supplemental experimental procedures (Table S3).