PMC:7544934 / 15411-16177 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7544934","sourcedb":"PMC","sourceid":"7544934","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7544934","text":"(A) LDN prevents LPS induced pro-inflammatory cytokines expression and release Quantitative mRNA expression of indicated genes (mcp-1, il-6 and Il1b) in murine macrophage cells. (B) ELISA of pro-inflammatory proteins (MCP-1, IL-6 and IL-1β) in conditioned media from LPS challenged murine macrophage cells in the present and absence of LDN (C) Quantitative mRNA expression of IL-1β, mcp-1 and IL-6 in purified ATMs from all group mice. Values are expressed as mean ± SEM (n = 3) from three independent sets of repeats (mean ± SEM ***p \u003c 0.001, **p, ^^p \u003c 0.01 *p,^p \u003c 0.05.) (D) LDN acts as EKR1 inhibitor and improved insulin sensitivity in LPS treated macrophage cells. ERK1/2 (Immunoblot) in RAW cells treated with LPS (1ug/ml) in the presence and absence of LDN.","tracks":[{"project":"LitCovid_Glycan-Motif-Structure","denotations":[{"id":"T41","span":{"begin":4,"end":7},"obj":"https://glytoucan.org/Structures/Glycans/G00057MO"},{"id":"T42","span":{"begin":4,"end":7},"obj":"https://glytoucan.org/Structures/Glycans/G31736BK"},{"id":"T43","span":{"begin":336,"end":339},"obj":"https://glytoucan.org/Structures/Glycans/G00057MO"},{"id":"T44","span":{"begin":336,"end":339},"obj":"https://glytoucan.org/Structures/Glycans/G31736BK"},{"id":"T45","span":{"begin":579,"end":582},"obj":"https://glytoucan.org/Structures/Glycans/G00057MO"},{"id":"T46","span":{"begin":579,"end":582},"obj":"https://glytoucan.org/Structures/Glycans/G31736BK"},{"id":"T47","span":{"begin":762,"end":765},"obj":"https://glytoucan.org/Structures/Glycans/G00057MO"},{"id":"T48","span":{"begin":762,"end":765},"obj":"https://glytoucan.org/Structures/Glycans/G31736BK"}],"attributes":[{"subj":"T41","pred":"source","obj":"LitCovid_Glycan-Motif-Structure"},{"subj":"T42","pred":"source","obj":"LitCovid_Glycan-Motif-Structure"},{"subj":"T43","pred":"source","obj":"LitCovid_Glycan-Motif-Structure"},{"subj":"T44","pred":"source","obj":"LitCovid_Glycan-Motif-Structure"},{"subj":"T45","pred":"source","obj":"LitCovid_Glycan-Motif-Structure"},{"subj":"T46","pred":"source","obj":"LitCovid_Glycan-Motif-Structure"},{"subj":"T47","pred":"source","obj":"LitCovid_Glycan-Motif-Structure"},{"subj":"T48","pred":"source","obj":"LitCovid_Glycan-Motif-Structure"}]},{"project":"LitCovid-PubTator","denotations":[{"id":"342","span":{"begin":135,"end":139},"obj":"Gene"},{"id":"343","span":{"begin":144,"end":148},"obj":"Gene"},{"id":"344","span":{"begin":225,"end":229},"obj":"Gene"},{"id":"345","span":{"begin":234,"end":239},"obj":"Gene"},{"id":"346","span":{"begin":376,"end":381},"obj":"Gene"},{"id":"347","span":{"begin":393,"end":397},"obj":"Gene"},{"id":"348","span":{"begin":619,"end":626},"obj":"Gene"},{"id":"349","span":{"begin":672,"end":678},"obj":"Gene"},{"id":"350","span":{"begin":153,"end":159},"obj":"Species"},{"id":"351","span":{"begin":282,"end":288},"obj":"Species"},{"id":"352","span":{"begin":430,"end":434},"obj":"Species"},{"id":"353","span":{"begin":695,"end":698},"obj":"CellLine"}],"attributes":[{"id":"A342","pred":"tao:has_database_id","subj":"342","obj":"Gene:16193"},{"id":"A343","pred":"tao:has_database_id","subj":"343","obj":"Gene:16176"},{"id":"A344","pred":"tao:has_database_id","subj":"344","obj":"Gene:16193"},{"id":"A345","pred":"tao:has_database_id","subj":"345","obj":"Gene:16175"},{"id":"A346","pred":"tao:has_database_id","subj":"346","obj":"Gene:16175"},{"id":"A347","pred":"tao:has_database_id","subj":"347","obj":"Gene:16193"},{"id":"A348","pred":"tao:has_database_id","subj":"348","obj":"Gene:3630"},{"id":"A349","pred":"tao:has_database_id","subj":"349","obj":"Gene:5595"},{"id":"A350","pred":"tao:has_database_id","subj":"350","obj":"Tax:10090"},{"id":"A351","pred":"tao:has_database_id","subj":"351","obj":"Tax:10090"},{"id":"A352","pred":"tao:has_database_id","subj":"352","obj":"Tax:10090"},{"id":"A353","pred":"tao:has_database_id","subj":"353","obj":"CVCL:F681"},{"subj":"342","pred":"source","obj":"LitCovid-PubTator"},{"subj":"343","pred":"source","obj":"LitCovid-PubTator"},{"subj":"344","pred":"source","obj":"LitCovid-PubTator"},{"subj":"345","pred":"source","obj":"LitCovid-PubTator"},{"subj":"346","pred":"source","obj":"LitCovid-PubTator"},{"subj":"347","pred":"source","obj":"LitCovid-PubTator"},{"subj":"348","pred":"source","obj":"LitCovid-PubTator"},{"subj":"349","pred":"source","obj":"LitCovid-PubTator"},{"subj":"350","pred":"source","obj":"LitCovid-PubTator"},{"subj":"351","pred":"source","obj":"LitCovid-PubTator"},{"subj":"352","pred":"source","obj":"LitCovid-PubTator"},{"subj":"353","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T120","span":{"begin":436,"end":671},"obj":"Sentence"},{"id":"T121","span":{"begin":672,"end":766},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T120","pred":"source","obj":"LitCovid-sentences"},{"subj":"T121","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid_Glycan-Motif-Structure","color":"#ecb593","default":true},{"id":"LitCovid-PubTator","color":"#9b93ec"},{"id":"LitCovid-sentences","color":"#a4ec93"}]}]}}