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PMC:7544934 / 12938-16177 JSONTXT

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LitCovid_Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T23 0-3 https://glytoucan.org/Structures/Glycans/G00057MO denotes LDN
T24 0-3 https://glytoucan.org/Structures/Glycans/G31736BK denotes LDN
T25 636-639 https://glytoucan.org/Structures/Glycans/G00057MO denotes LDN
T26 636-639 https://glytoucan.org/Structures/Glycans/G31736BK denotes LDN
T27 660-663 https://glytoucan.org/Structures/Glycans/G00057MO denotes LDN
T28 660-663 https://glytoucan.org/Structures/Glycans/G31736BK denotes LDN
T29 988-991 https://glytoucan.org/Structures/Glycans/G00057MO denotes LDN
T30 988-991 https://glytoucan.org/Structures/Glycans/G31736BK denotes LDN
T31 1231-1234 https://glytoucan.org/Structures/Glycans/G00057MO denotes LDN
T32 1231-1234 https://glytoucan.org/Structures/Glycans/G31736BK denotes LDN
T33 1501-1504 https://glytoucan.org/Structures/Glycans/G00057MO denotes LDN
T34 1501-1504 https://glytoucan.org/Structures/Glycans/G31736BK denotes LDN
T35 1679-1682 https://glytoucan.org/Structures/Glycans/G00057MO denotes LDN
T36 1679-1682 https://glytoucan.org/Structures/Glycans/G31736BK denotes LDN
T37 2271-2274 https://glytoucan.org/Structures/Glycans/G00057MO denotes LDN
T38 2271-2274 https://glytoucan.org/Structures/Glycans/G31736BK denotes LDN
T39 2378-2381 https://glytoucan.org/Structures/Glycans/G00057MO denotes LDN
T40 2378-2381 https://glytoucan.org/Structures/Glycans/G31736BK denotes LDN
T41 2477-2480 https://glytoucan.org/Structures/Glycans/G00057MO denotes LDN
T42 2477-2480 https://glytoucan.org/Structures/Glycans/G31736BK denotes LDN
T43 2809-2812 https://glytoucan.org/Structures/Glycans/G00057MO denotes LDN
T44 2809-2812 https://glytoucan.org/Structures/Glycans/G31736BK denotes LDN
T45 3052-3055 https://glytoucan.org/Structures/Glycans/G00057MO denotes LDN
T46 3052-3055 https://glytoucan.org/Structures/Glycans/G31736BK denotes LDN
T47 3235-3238 https://glytoucan.org/Structures/Glycans/G00057MO denotes LDN
T48 3235-3238 https://glytoucan.org/Structures/Glycans/G31736BK denotes LDN

LitCovid-PD-HP

Id Subject Object Predicate Lexical cue hp_id
T5 37-51 Phenotype denotes cytokine storm http://purl.obolibrary.org/obo/HP_0033041
T6 52-66 Phenotype denotes Cytokine storm http://purl.obolibrary.org/obo/HP_0033041
T7 333-347 Phenotype denotes cytokine storm http://purl.obolibrary.org/obo/HP_0033041
T8 434-440 Phenotype denotes sepsis http://purl.obolibrary.org/obo/HP_0100806

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
281 0-3 Chemical denotes LDN
292 126-134 Species denotes patients Tax:9606
293 293-301 Species denotes patients Tax:9606
294 504-510 Species denotes murine Tax:10090
295 400-418 Chemical denotes lipopolysaccharide MESH:D008070
296 729-732 Chemical denotes MTT MESH:C070243
297 734-790 Chemical denotes 4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide
298 117-125 Disease denotes COVID-19 MESH:C000657245
299 173-182 Disease denotes mortality MESH:D003643
300 284-292 Disease denotes COVID-19 MESH:C000657245
301 434-440 Disease denotes sepsis MESH:D018805
316 956-961 Gene denotes IL-1β Gene:16175
317 963-967 Gene denotes IL-6 Gene:16193
318 1034-1039 Gene denotes IL-1β Gene:16175
319 1041-1045 Gene denotes IL-6 Gene:16193
320 1446-1451 Gene denotes IL-1β Gene:16175
321 1463-1467 Gene denotes IL-6 Gene:16193
322 1538-1543 Gene denotes IL-1β Gene:16175
323 1554-1558 Gene denotes IL-6 Gene:16193
324 2215-2220 Gene denotes IL-1β Gene:16175
325 2232-2236 Gene denotes IL-6 Gene:16193
326 1501-1504 Chemical denotes LDN
327 1679-1682 Chemical denotes LDN
328 2378-2381 Chemical denotes LDN
329 2448-2460 Disease denotes inflammation MESH:D007249
342 2608-2612 Gene denotes il-6 Gene:16193
343 2617-2621 Gene denotes Il1b Gene:16176
344 2698-2702 Gene denotes IL-6 Gene:16193
345 2707-2712 Gene denotes IL-1β Gene:16175
346 2849-2854 Gene denotes IL-1β Gene:16175
347 2866-2870 Gene denotes IL-6 Gene:16193
348 3092-3099 Gene denotes insulin Gene:3630
349 3145-3151 Gene denotes ERK1/2 Gene:5595
350 2626-2632 Species denotes murine Tax:10090
351 2755-2761 Species denotes murine Tax:10090
352 2903-2907 Species denotes mice Tax:10090
353 3168-3171 CellLine denotes RAW CVCL:F681

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T107 0-51 Sentence denotes LDN treatment diminishes LPS induced cytokine storm
T108 52-256 Sentence denotes Cytokine storm is a very commonly observed factor in most severe COVID-19 patients and also one of the leading causes of mortality (Coperchini et al., 2020; Rahmati and Moosavi, 2020; Zhang et al., 2020).
T109 257-368 Sentence denotes Peripheral blood of severe COVID-19 patients has also shown a high level of cytokine storm (Wu and Yang, 2020).
T110 369-647 Sentence denotes Keeping in mind the ability of lipopolysaccharide (LPS) to cause sepsis and triggers an uncontrolled systemic inflammatory response in murine macrophage cells (Ramos-Benitez et al., 2018), we treated macrophage cells with LPS (1 µg/ml) in the presence and absence of LDN (5 µM).
T111 648-851 Sentence denotes The dose of LDN chosen is non-toxic (Figure S2) as found in cell viability using MTT (4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide) assay following the standard protocol (Dogra et al., 2019).
T112 852-1090 Sentence denotes Results demonstrated that LPS treatment significantly induced expression on pro-inflammatory cytokines (IL-1β, IL-6 and mcp-1) whereas, LDN significantly inhibited LPS expression of IL-1β, IL-6 and mcp-1 in macrophage cells (Figure 1(A)).
T113 1091-1322 Sentence denotes Next, we tested the possible involvement of LPS in inducing the release of pro-inflammatory cytokines, and we also determine the effects of LDN on release of pro-inflammatory mediators in LPS induced macrophage cells (Figure 1(B)).
T114 1323-1579 Sentence denotes Conditioned media from LPS challenged macrophage cells showed a significantly enhanced release pro-inflammatory mediators (IL-1β, MCP-1 and IL-6) and interestingly we found that LDN treatment attenuated LPS induced IL-1β, MCP1 and IL-6 level (Figure 1(B)).
T115 1580-1779 Sentence denotes These data suggest that LPS induced macrophage cells, to release - pro-inflammatory mediators, and LDN treatment can significantly abrogate LPS induced release of pro-inflammatory mediators in media.
T116 1780-2056 Sentence denotes Adipose tissue macrophages (ATMs) is closely linked to this inflammatory condition which leads to numbers of diseases and the ability of High Fat Diet (HFD) feeding on increased LPS uptake and trafficking to macrophages and other targets are well known (Hersoug et al., 2016).
T117 2057-2336 Sentence denotes Hence in this study, we investigated the effect of HFD on proinflammatory markers expression in purified ATMs. Expression of pro-inflammatory markers such as IL-1β, MCP-1 and IL-6 was induced (Figure 1(C)) whereas LDN attenuates HFD induced pro-inflammatory cytokines expression.
T118 2337-2461 Sentence denotes Altogether, this data clearly shows that LDN treatment may protect (by reducing elevated M1 cytokines) against inflammation.
T119 2462-2908 Sentence denotes Figure 1. (A) LDN prevents LPS induced pro-inflammatory cytokines expression and release Quantitative mRNA expression of indicated genes (mcp-1, il-6 and Il1b) in murine macrophage cells. (B) ELISA of pro-inflammatory proteins (MCP-1, IL-6 and IL-1β) in conditioned media from LPS challenged murine macrophage cells in the present and absence of LDN (C) Quantitative mRNA expression of IL-1β, mcp-1 and IL-6 in purified ATMs from all group mice.
T120 2909-3144 Sentence denotes Values are expressed as mean ± SEM (n = 3) from three independent sets of repeats (mean ± SEM ***p < 0.001, **p, ^^p < 0.01 *p,^p < 0.05.) (D) LDN acts as EKR1 inhibitor and improved insulin sensitivity in LPS treated macrophage cells.
T121 3145-3239 Sentence denotes ERK1/2 (Immunoblot) in RAW cells treated with LPS (1ug/ml) in the presence and absence of LDN.

2_test

Id Subject Object Predicate Lexical cue
32930058-26712364-56195697 2050-2054 26712364 denotes 2016