PMC:7543426 / 6114-6769 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T39","span":{"begin":78,"end":82},"obj":"Body_part"},{"id":"T40","span":{"begin":162,"end":166},"obj":"Body_part"},{"id":"T41","span":{"begin":167,"end":174},"obj":"Body_part"}],"attributes":[{"id":"A39","pred":"fma_id","subj":"T39","obj":"http://purl.org/sig/ont/fma/fma7195"},{"id":"A40","pred":"fma_id","subj":"T40","obj":"http://purl.org/sig/ont/fma/fma7195"},{"id":"A41","pred":"fma_id","subj":"T41","obj":"http://purl.org/sig/ont/fma/fma9637"}],"text":"Transmission Electron Microscopy\nWe only looked for evidence of SARS-CoV-2 in lung samples. Nonlung samples were not subject to transmission electron microscopy. Lung tissues were fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer for 4 hours at 4°C. Fixation was performed with 1% OsO4. Samples were then dehydrated in graded ethanol and embedded in Epon resin, as previously described [14, 15]. Ultrathin sections were stained with 2% uranyl acetate and examined under a transmission electron microscope (JEOL JEM 2100 Plus; Japan Electron Optics Laboratory). Images were captured with a digital camera (Tietz Video and Image Processing Systems)."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T33","span":{"begin":78,"end":82},"obj":"Body_part"},{"id":"T34","span":{"begin":162,"end":166},"obj":"Body_part"}],"attributes":[{"id":"A33","pred":"uberon_id","subj":"T33","obj":"http://purl.obolibrary.org/obo/UBERON_0002048"},{"id":"A34","pred":"uberon_id","subj":"T34","obj":"http://purl.obolibrary.org/obo/UBERON_0002048"}],"text":"Transmission Electron Microscopy\nWe only looked for evidence of SARS-CoV-2 in lung samples. Nonlung samples were not subject to transmission electron microscopy. Lung tissues were fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer for 4 hours at 4°C. Fixation was performed with 1% OsO4. Samples were then dehydrated in graded ethanol and embedded in Epon resin, as previously described [14, 15]. Ultrathin sections were stained with 2% uranyl acetate and examined under a transmission electron microscope (JEOL JEM 2100 Plus; Japan Electron Optics Laboratory). Images were captured with a digital camera (Tietz Video and Image Processing Systems)."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T53","span":{"begin":64,"end":72},"obj":"Disease"}],"attributes":[{"id":"A53","pred":"mondo_id","subj":"T53","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"Transmission Electron Microscopy\nWe only looked for evidence of SARS-CoV-2 in lung samples. Nonlung samples were not subject to transmission electron microscopy. Lung tissues were fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer for 4 hours at 4°C. Fixation was performed with 1% OsO4. Samples were then dehydrated in graded ethanol and embedded in Epon resin, as previously described [14, 15]. Ultrathin sections were stained with 2% uranyl acetate and examined under a transmission electron microscope (JEOL JEM 2100 Plus; Japan Electron Optics Laboratory). Images were captured with a digital camera (Tietz Video and Image Processing Systems)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T87","span":{"begin":78,"end":82},"obj":"http://purl.obolibrary.org/obo/UBERON_0002048"},{"id":"T88","span":{"begin":78,"end":82},"obj":"http://www.ebi.ac.uk/efo/EFO_0000934"},{"id":"T89","span":{"begin":162,"end":166},"obj":"http://purl.obolibrary.org/obo/UBERON_0002048"},{"id":"T90","span":{"begin":162,"end":166},"obj":"http://www.ebi.ac.uk/efo/EFO_0000934"},{"id":"T91","span":{"begin":253,"end":256},"obj":"http://purl.obolibrary.org/obo/CLO_0001387"},{"id":"T92","span":{"begin":478,"end":479},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T93","span":{"begin":595,"end":596},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T94","span":{"begin":597,"end":604},"obj":"http://www.ebi.ac.uk/efo/EFO_0000881"}],"text":"Transmission Electron Microscopy\nWe only looked for evidence of SARS-CoV-2 in lung samples. Nonlung samples were not subject to transmission electron microscopy. Lung tissues were fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer for 4 hours at 4°C. Fixation was performed with 1% OsO4. Samples were then dehydrated in graded ethanol and embedded in Epon resin, as previously described [14, 15]. Ultrathin sections were stained with 2% uranyl acetate and examined under a transmission electron microscope (JEOL JEM 2100 Plus; Japan Electron Optics Laboratory). Images were captured with a digital camera (Tietz Video and Image Processing Systems)."}

{"project":"LitCovid-PubTator","denotations":[{"id":"178","span":{"begin":64,"end":74},"obj":"Species"},{"id":"179","span":{"begin":196,"end":210},"obj":"Chemical"},{"id":"180","span":{"begin":220,"end":237},"obj":"Chemical"},{"id":"181","span":{"begin":289,"end":293},"obj":"Chemical"},{"id":"182","span":{"begin":334,"end":341},"obj":"Chemical"},{"id":"183","span":{"begin":444,"end":458},"obj":"Chemical"}],"attributes":[{"id":"A178","pred":"tao:has_database_id","subj":"178","obj":"Tax:2697049"},{"id":"A179","pred":"tao:has_database_id","subj":"179","obj":"MESH:D005976"},{"id":"A182","pred":"tao:has_database_id","subj":"182","obj":"MESH:D000431"},{"id":"A183","pred":"tao:has_database_id","subj":"183","obj":"MESH:C005460"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Transmission Electron Microscopy\nWe only looked for evidence of SARS-CoV-2 in lung samples. Nonlung samples were not subject to transmission electron microscopy. Lung tissues were fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer for 4 hours at 4°C. Fixation was performed with 1% OsO4. Samples were then dehydrated in graded ethanol and embedded in Epon resin, as previously described [14, 15]. Ultrathin sections were stained with 2% uranyl acetate and examined under a transmission electron microscope (JEOL JEM 2100 Plus; Japan Electron Optics Laboratory). Images were captured with a digital camera (Tietz Video and Image Processing Systems)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T52","span":{"begin":0,"end":32},"obj":"Sentence"},{"id":"T53","span":{"begin":33,"end":91},"obj":"Sentence"},{"id":"T54","span":{"begin":92,"end":161},"obj":"Sentence"},{"id":"T55","span":{"begin":162,"end":257},"obj":"Sentence"},{"id":"T56","span":{"begin":258,"end":294},"obj":"Sentence"},{"id":"T57","span":{"begin":295,"end":403},"obj":"Sentence"},{"id":"T58","span":{"begin":404,"end":568},"obj":"Sentence"},{"id":"T59","span":{"begin":569,"end":655},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Transmission Electron Microscopy\nWe only looked for evidence of SARS-CoV-2 in lung samples. Nonlung samples were not subject to transmission electron microscopy. Lung tissues were fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer for 4 hours at 4°C. Fixation was performed with 1% OsO4. Samples were then dehydrated in graded ethanol and embedded in Epon resin, as previously described [14, 15]. Ultrathin sections were stained with 2% uranyl acetate and examined under a transmission electron microscope (JEOL JEM 2100 Plus; Japan Electron Optics Laboratory). Images were captured with a digital camera (Tietz Video and Image Processing Systems)."}

{"project":"2_test","denotations":[{"id":"32914853-31217430-61098655","span":{"begin":395,"end":397},"obj":"31217430"},{"id":"32914853-24813622-61098656","span":{"begin":399,"end":401},"obj":"24813622"}],"text":"Transmission Electron Microscopy\nWe only looked for evidence of SARS-CoV-2 in lung samples. Nonlung samples were not subject to transmission electron microscopy. Lung tissues were fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer for 4 hours at 4°C. Fixation was performed with 1% OsO4. Samples were then dehydrated in graded ethanol and embedded in Epon resin, as previously described [14, 15]. Ultrathin sections were stained with 2% uranyl acetate and examined under a transmission electron microscope (JEOL JEM 2100 Plus; Japan Electron Optics Laboratory). Images were captured with a digital camera (Tietz Video and Image Processing Systems)."}