PMC:7443692 / 8892-9630
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7443692","sourcedb":"PMC","sourceid":"7443692","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7443692","text":"ch dataset, along with existing 3D-structures of both glycoproteins, to generate static and molecular dynamics (MD) models of S alone, and in complex with the glycosylated, soluble ACE2 receptor. By combining bioinformatics characterization of viral evolution and variants of S and ACE2 with MD simulations of the glycosylated S-ACE2 interaction, we identified important roles for glycans in multiple processes, including receptor-viral binding and glycan shielding of S. Our rich characterization of the recombinant, glycosylated S trimer mimetic immunogen of SARS-CoV-2 in complex with the soluble human ACE2 receptor provides a detailed platform for guiding rational vaccine, antibody, and inhibitor design.\n\nResults\n\nExpression, Purif","divisions":[{"label":"title","span":{"begin":712,"end":719}}],"tracks":[{"project":"LitCovid-sample-CHEBI","denotations":[{"id":"T36","span":{"begin":54,"end":67},"obj":"Chemical"},{"id":"T37","span":{"begin":381,"end":388},"obj":"Chemical"}],"attributes":[{"id":"A36","pred":"chebi_id","subj":"T36","obj":"http://purl.obolibrary.org/obo/CHEBI_17089"},{"id":"A37","pred":"chebi_id","subj":"T37","obj":"http://purl.obolibrary.org/obo/CHEBI_18154"},{"subj":"T36","pred":"source","obj":"LitCovid-sample-CHEBI"},{"subj":"T37","pred":"source","obj":"LitCovid-sample-CHEBI"}]},{"project":"LitCovid-sample-PD-NCBITaxon","denotations":[{"id":"T38","span":{"begin":561,"end":571},"obj":"Species"},{"id":"T39","span":{"begin":600,"end":605},"obj":"Species"}],"attributes":[{"id":"A38","pred":"ncbi_taxonomy_id","subj":"T38","obj":"NCBItxid:2697049"},{"id":"A39","pred":"ncbi_taxonomy_id","subj":"T39","obj":"NCBItxid:9606"},{"subj":"T38","pred":"source","obj":"LitCovid-sample-PD-NCBITaxon"},{"subj":"T39","pred":"source","obj":"LitCovid-sample-PD-NCBITaxon"}],"namespaces":[{"prefix":"NCBItxid","uri":"http://purl.bioontology.org/ontology/NCBITAXON/"}]},{"project":"LitCovid-sample-sentences","denotations":[{"id":"T46","span":{"begin":196,"end":471},"obj":"Sentence"},{"id":"T47","span":{"begin":472,"end":710},"obj":"Sentence"},{"id":"T48","span":{"begin":712,"end":719},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T46","pred":"source","obj":"LitCovid-sample-sentences"},{"subj":"T47","pred":"source","obj":"LitCovid-sample-sentences"},{"subj":"T48","pred":"source","obj":"LitCovid-sample-sentences"}]},{"project":"LitCovid-sample-PD-MONDO","denotations":[{"id":"T29","span":{"begin":561,"end":571},"obj":"Disease"}],"attributes":[{"id":"A29","pred":"mondo_id","subj":"T29","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"},{"subj":"T29","pred":"source","obj":"LitCovid-sample-PD-MONDO"}]},{"project":"LitCovid-sample-UniProt","denotations":[{"id":"T1102","span":{"begin":54,"end":67},"obj":"Protein"},{"id":"T1187","span":{"begin":181,"end":185},"obj":"Protein"},{"id":"T1188","span":{"begin":282,"end":286},"obj":"Protein"},{"id":"T1189","span":{"begin":329,"end":333},"obj":"Protein"},{"id":"T1190","span":{"begin":606,"end":610},"obj":"Protein"}],"attributes":[{"id":"A1102","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q9QSP1"},{"id":"A1103","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q9QJT6"},{"id":"A1104","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q9JGT4"},{"id":"A1105","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q9IPJ6"},{"id":"A1106","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q9DIC6"},{"id":"A1107","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q91DS0"},{"id":"A1108","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q91C28"},{"id":"A1109","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q8QQA2"},{"id":"A1110","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q8QPE5"},{"id":"A1111","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q8QPE4"},{"id":"A1112","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q8QPE3"},{"id":"A1113","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q8JTH0"},{"id":"A1114","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q8BDV6"},{"id":"A1115","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q8B6J6"},{"id":"A1116","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q8B0I1"},{"id":"A1117","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q8B0H6"},{"id":"A1118","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q8B0H1"},{"id":"A1119","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q89669"},{"id":"A1120","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q85213"},{"id":"A1121","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q82706"},{"id":"A1122","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q82683"},{"id":"A1123","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q82020"},{"id":"A1124","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q787B5"},{"id":"A1125","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q77SK0"},{"id":"A1126","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q77N36"},{"id":"A1127","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q76G52"},{"id":"A1128","pred":"uniprot_id","subj":"T1102","obj":"https://www.uniprot.org/uniprot/Q75T09"},{"id":"A1129","pred":"unipro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